In Vitro Evaluation of Monohalogenated Semicarbazones and Thiosemicarbazones as Potential Cytotoxic Agents Induction of Apoptosis and Genotoxicity
A series of halogenated Semicarbazones (SCs) and Thiosemicarbazones (TSCs) (11-30) were synthesized from mono fluorinated-, bromine- and chlorinated acetophenones (1-10). Structures were confirmed by Nuclear Magnetic Resonance (NMR) spectral data. Both effects, the halogenated substituent and the po...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/88094 |
| Acceso en línea: | http://hdl.handle.net/11336/88094 |
| Access Level: | acceso abierto |
| Palabra clave: | M-FLUOROSEMICARBAZONES M-FLUOROTHIOSEMICARBAZONES CYTOTOXIC ACTIVITY APOPTOSIS INDUCTION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | A series of halogenated Semicarbazones (SCs) and Thiosemicarbazones (TSCs) (11-30) were synthesized from mono fluorinated-, bromine- and chlorinated acetophenones (1-10). Structures were confirmed by Nuclear Magnetic Resonance (NMR) spectral data. Both effects, the halogenated substituent and the position of the substitution on the antiproliferative activity, were systematically investigated for the first time. Cytotoxic activity was evaluated, using tetrazolium salt method (MTT), in two murine cell lines: CT26 (colon cancer) and B16 (melanoma). Only, o-, m- and p-fluorinated SCs and TSCs showed significant cytotoxic activity. Among them, compounds with fluorine at m-position in thephenyl ring showed the superior antiproliferative activity. The most actives derivatives were: m-Fluoroacetophenone semicarbazone (13) (μM; IC50 =7.2 ± 0.5, IC50=8.1 ± 0.2) and m-Fluoroacetophenone Thiosemicarbazone (23) (μM; IC50 = 3.1 ± 0.4, IC50=4.9 ± 0.5) in CT26 and B16, respectively. In addition, studying the genes Bcl-2 and Bax, compound 23 showed apoptosis induction and non-genotoxic properties. |
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