Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)

Scaldaferro, Marisel Analía; Grabiele, Mauro; Seijo, Jose Guillermo; Debat, Humberto Julio; Romero, María Victoria; Ducasse, Daniel Adrian; Prina, Alberto Raul; Moscone, Eduardo Alberto

Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)

Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromos...

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Autores:	Scaldaferro, Marisel Analía, Grabiele, Mauro, Seijo, Jose Guillermo, Debat, Humberto Julio, Romero, María Victoria, Ducasse, Daniel Adrian, Prina, Alberto Raul, Moscone, Eduardo Alberto
Tipo de recurso:	artículo
Estado:	Versión aceptada para publicación
Fecha de publicación:	2014
País:	Argentina
Institución:	Instituto Nacional de Tecnología Agropecuaria
Repositorio:	INTA Digital (INTA)
Idioma:	inglés
OAI Identifier:	oai:localhost:20.500.12123/2242
Acceso en línea:	https://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064 http://hdl.handle.net/20.500.12123/2242 https://doi.org/10.3109/09553002.2013.825064
Access Level:	acceso abierto
Palabra clave:	Capsicum baccatum Guindilla Cromosomas Citogenética Radiación Ionizante Chillies Chromosomes Cytogenetics Ionizing Radiation Capsicum pendulum

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dc.title.none.fl_str_mv	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
title	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
spellingShingle	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) Scaldaferro, Marisel Analía Capsicum baccatum Guindilla Cromosomas Citogenética Radiación Ionizante Chillies Chromosomes Cytogenetics Ionizing Radiation Capsicum pendulum
title_short	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
title_full	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
title_fullStr	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
title_full_unstemmed	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
title_sort	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
dc.creator.none.fl_str_mv	Scaldaferro, Marisel Analía Grabiele, Mauro Seijo, Jose Guillermo Debat, Humberto Julio Romero, María Victoria Ducasse, Daniel Adrian Prina, Alberto Raul Moscone, Eduardo Alberto
author	Scaldaferro, Marisel Analía
author_facet	Scaldaferro, Marisel Analía Grabiele, Mauro Seijo, Jose Guillermo Debat, Humberto Julio Romero, María Victoria Ducasse, Daniel Adrian Prina, Alberto Raul Moscone, Eduardo Alberto
author_role	author
author2	Grabiele, Mauro Seijo, Jose Guillermo Debat, Humberto Julio Romero, María Victoria Ducasse, Daniel Adrian Prina, Alberto Raul Moscone, Eduardo Alberto
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Capsicum baccatum Guindilla Cromosomas Citogenética Radiación Ionizante Chillies Chromosomes Cytogenetics Ionizing Radiation Capsicum pendulum
topic	Capsicum baccatum Guindilla Cromosomas Citogenética Radiación Ionizante Chillies Chromosomes Cytogenetics Ionizing Radiation Capsicum pendulum
description	Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromosome aberrations were analysed by different cytogenetic methods [Feulgen, silver staining for nucleolus organizer regions (silver positive nucleolus organizing regions or AgNOR), fluorescent banding, fluorescence in situ hybridization (FISH) and meiotic analysis]. Results: A rearranged chromosome carrying two nucleolus organizing regions (NOR) induced by ionizing radiation was detected in the cultivar, with the occurrence of a small reciprocal exchange between a chromosome of pair no. 1 and another chromosome of pair no. 3, both carrying active NOR in short arms and associated chromomycin A positive/diamidino-phenylindole negative (CMA+/DAPI−) heterochromatin. Meiotic analysis showed a quadrivalent configuration, confirming a reciprocal translocation between two chromosomes. Conclusions: The use of X-rays in Capsicum allowed us to develop and identify a pepper line with structural rearrangements between two NOR-carrying chromosomes. We postulate that all the cytological techniques employed in this research were efficient in the search for chromosome aberrations. Particularly, Feulgen and AgNOR were the most suitable in those cases of transient rearrangements, whereas fluorescent banding and FISH were appropriate for intransitive ones.
publishDate	2014
dc.date.none.fl_str_mv	2014 2018-04-13T13:35:48Z 2018-04-13T13:35:48Z
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064 http://hdl.handle.net/20.500.12123/2242 0955-3002 1362-3095 https://doi.org/10.3109/09553002.2013.825064
url	https://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064 http://hdl.handle.net/20.500.12123/2242 https://doi.org/10.3109/09553002.2013.825064
identifier_str_mv	0955-3002 1362-3095
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv	openAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	International Journal of Radiation Biology 90 (1) : 104-112 (2014) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
instname_str	Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
collection	INTA Digital (INTA)
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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spelling	Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)Scaldaferro, Marisel AnalíaGrabiele, MauroSeijo, Jose GuillermoDebat, Humberto JulioRomero, María VictoriaDucasse, Daniel AdrianPrina, Alberto RaulMoscone, Eduardo AlbertoCapsicum baccatumGuindillaCromosomasCitogenéticaRadiación IonizanteChilliesChromosomesCytogeneticsIonizing RadiationCapsicum pendulumPurpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromosome aberrations were analysed by different cytogenetic methods [Feulgen, silver staining for nucleolus organizer regions (silver positive nucleolus organizing regions or AgNOR), fluorescent banding, fluorescence in situ hybridization (FISH) and meiotic analysis]. Results: A rearranged chromosome carrying two nucleolus organizing regions (NOR) induced by ionizing radiation was detected in the cultivar, with the occurrence of a small reciprocal exchange between a chromosome of pair no. 1 and another chromosome of pair no. 3, both carrying active NOR in short arms and associated chromomycin A positive/diamidino-phenylindole negative (CMA+/DAPI−) heterochromatin. Meiotic analysis showed a quadrivalent configuration, confirming a reciprocal translocation between two chromosomes. Conclusions: The use of X-rays in Capsicum allowed us to develop and identify a pepper line with structural rearrangements between two NOR-carrying chromosomes. We postulate that all the cytological techniques employed in this research were efficient in the search for chromosome aberrations. Particularly, Feulgen and AgNOR were the most suitable in those cases of transient rearrangements, whereas fluorescent banding and FISH were appropriate for intransitive ones.Instituto de Patología VegetalInstituto de GenéticaFil: Scaldaferro, Marisel Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste; ArgentinaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Romero, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Moscone, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina2018-04-13T13:35:48Z2018-04-13T13:35:48Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064http://hdl.handle.net/20.500.12123/22420955-30021362-3095https://doi.org/10.3109/09553002.2013.825064International Journal of Radiation Biology 90 (1) : 104-112 (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2024-05-15T12:23:38Zoai:localhost:20.500.12123/2242instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2024-05-15 12:23:38.494INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
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Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)

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