Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy
The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an ob...
| Autores: | , , , , , , , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2022 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/218370 |
| Acesso em linha: | http://hdl.handle.net/11336/218370 |
| Access Level: | Acceso aberto |
| Palavra-chave: | ENDOGENOUS GHSR ANTAGONIST GHRELIN/LEAP-2 RATIO PREGNANCY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
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| dc.title.none.fl_str_mv |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| spellingShingle |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy Garcés, Maria Fernanda ENDOGENOUS GHSR ANTAGONIST GHRELIN/LEAP-2 RATIO PREGNANCY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| title_short |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_full |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_fullStr |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_full_unstemmed |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| title_sort |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy |
| dc.creator.none.fl_str_mv |
Garcés, Maria Fernanda Buell Acosta, Julieth Daniela Ángel Müller, Edith Parada Baños, Arturo José Acosta Alvarez, Jaidy Saavedra López, Harold Felipe Franco Vega, Roberto Maldonado Acosta, Luis Miguel Escobar Cordoba, Franklin Vásquez Romero, Keydy Lacunza, Ezequiel Caminos Cepeda, Sofía Alexandra Nogueiras, Rubén Diéguez, Carlos Ruiz Parra, Ariel Iván Caminos, Jorge Eduardo |
| author |
Garcés, Maria Fernanda |
| author_facet |
Garcés, Maria Fernanda Buell Acosta, Julieth Daniela Ángel Müller, Edith Parada Baños, Arturo José Acosta Alvarez, Jaidy Saavedra López, Harold Felipe Franco Vega, Roberto Maldonado Acosta, Luis Miguel Escobar Cordoba, Franklin Vásquez Romero, Keydy Lacunza, Ezequiel Caminos Cepeda, Sofía Alexandra Nogueiras, Rubén Diéguez, Carlos Ruiz Parra, Ariel Iván Caminos, Jorge Eduardo |
| author_role |
author |
| author2 |
Buell Acosta, Julieth Daniela Ángel Müller, Edith Parada Baños, Arturo José Acosta Alvarez, Jaidy Saavedra López, Harold Felipe Franco Vega, Roberto Maldonado Acosta, Luis Miguel Escobar Cordoba, Franklin Vásquez Romero, Keydy Lacunza, Ezequiel Caminos Cepeda, Sofía Alexandra Nogueiras, Rubén Diéguez, Carlos Ruiz Parra, Ariel Iván Caminos, Jorge Eduardo |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
ENDOGENOUS GHSR ANTAGONIST GHRELIN/LEAP-2 RATIO PREGNANCY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic |
ENDOGENOUS GHSR ANTAGONIST GHRELIN/LEAP-2 RATIO PREGNANCY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| description |
The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an observational prospective cohort. Healthy and mild preeclamptic pregnant women were studied at each trimester of gestation and three months postpartum. In addition, a group of non-pregnant women was studied into the follicular and luteal phases of the menstrual cycle. Furthermore, Ghrelin/LEAP-2 ratio was investigated in non-pregnant rats and at different periods of rat pregnancy. Human and rat serum ghrelin and LEAP-2 levels were determined using the commercially available ELISA kits. The Ghrelin/LEAP-2 ratio peak around the second trimester of gestation in healthy pregnant women (p < 0.05). Additionally, there were no statistically significant differences in Ghrelin/LEAP-2 ratio between healthy and preeclamptic pregnant women at each trimester of gestation (p > 0.05). The Ghrelin/LEAP-2 ratio in pregnant rat reached the peak around mid-gestation with a similar pattern to the human pregnancy. LEAP-2 was visualized by immunohistochemistry in human term placenta and rat placentas on days 12, 16 and 21 of pregnancy. In conclusion, this study provides the first evidence of a Ghrelin/LEAP-2 ratio peak around the half-way point of pregnancy onwards during human and rat pregnancy, and it might be associated with increased rates of weight gain during pregnancy. Thus, this study suggests that LEAP-2 and Ghrelin/LEAP-2 ratio might play an important role in maternal physiology adaptation of weight gain during pregnancy. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-09 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/218370 Garcés, Maria Fernanda; Buell Acosta, Julieth Daniela; Ángel Müller, Edith; Parada Baños, Arturo José; Acosta Alvarez, Jaidy; et al.; Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 23; 17; 9-2022; 1-12 1661-6596 1422-0067 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/218370 |
| identifier_str_mv |
Garcés, Maria Fernanda; Buell Acosta, Julieth Daniela; Ángel Müller, Edith; Parada Baños, Arturo José; Acosta Alvarez, Jaidy; et al.; Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 23; 17; 9-2022; 1-12 1661-6596 1422-0067 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms23179514 info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/17/9514 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
| publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
| repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
| _version_ |
1799195062996828160 |
| spelling |
Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancyGarcés, Maria FernandaBuell Acosta, Julieth DanielaÁngel Müller, EdithParada Baños, Arturo JoséAcosta Alvarez, JaidySaavedra López, Harold FelipeFranco Vega, RobertoMaldonado Acosta, Luis MiguelEscobar Cordoba, FranklinVásquez Romero, KeydyLacunza, EzequielCaminos Cepeda, Sofía AlexandraNogueiras, RubénDiéguez, CarlosRuiz Parra, Ariel IvánCaminos, Jorge EduardoENDOGENOUS GHSR ANTAGONISTGHRELIN/LEAP-2 RATIOPREGNANCYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Liver-Expressed Antimicrobial Peptide 2 (LEAP-2) has emerged as an endogenous GHS-R antagonist and blunts the orexigenic action of ghrelin. This study aimed to determine the Ghrelin/LEAP-2 ratio in humans and rats during pregnancy. In humans, we conducted a nested case-control study within an observational prospective cohort. Healthy and mild preeclamptic pregnant women were studied at each trimester of gestation and three months postpartum. In addition, a group of non-pregnant women was studied into the follicular and luteal phases of the menstrual cycle. Furthermore, Ghrelin/LEAP-2 ratio was investigated in non-pregnant rats and at different periods of rat pregnancy. Human and rat serum ghrelin and LEAP-2 levels were determined using the commercially available ELISA kits. The Ghrelin/LEAP-2 ratio peak around the second trimester of gestation in healthy pregnant women (p < 0.05). Additionally, there were no statistically significant differences in Ghrelin/LEAP-2 ratio between healthy and preeclamptic pregnant women at each trimester of gestation (p > 0.05). The Ghrelin/LEAP-2 ratio in pregnant rat reached the peak around mid-gestation with a similar pattern to the human pregnancy. LEAP-2 was visualized by immunohistochemistry in human term placenta and rat placentas on days 12, 16 and 21 of pregnancy. In conclusion, this study provides the first evidence of a Ghrelin/LEAP-2 ratio peak around the half-way point of pregnancy onwards during human and rat pregnancy, and it might be associated with increased rates of weight gain during pregnancy. Thus, this study suggests that LEAP-2 and Ghrelin/LEAP-2 ratio might play an important role in maternal physiology adaptation of weight gain during pregnancy.Fil: Garcés, Maria Fernanda. Universidad Nacional de Colombia; ColombiaFil: Buell Acosta, Julieth Daniela. Universidad Nacional de Colombia; ColombiaFil: Ángel Müller, Edith. Universidad Nacional de Colombia; ColombiaFil: Parada Baños, Arturo José. Universidad Nacional de Colombia; ColombiaFil: Acosta Alvarez, Jaidy. Universidad Nacional de Colombia; ColombiaFil: Saavedra López, Harold Felipe. Universidad Nacional de Colombia; ColombiaFil: Franco Vega, Roberto. Universidad Nacional de Colombia; ColombiaFil: Maldonado Acosta, Luis Miguel. Universidad Nacional de Colombia; ColombiaFil: Escobar Cordoba, Franklin. Universidad Nacional de Colombia; ColombiaFil: Vásquez Romero, Keydy. Universidad de los Andes; ColombiaFil: Lacunza, Ezequiel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Caminos Cepeda, Sofía Alexandra. Universitat Pompeu Fabra; EspañaFil: Nogueiras, Rubén. Universidad de Santiago de Compostela; EspañaFil: Diéguez, Carlos. Universidad de Santiago de Compostela; EspañaFil: Ruiz Parra, Ariel Iván. Universidad Nacional de Colombia; ColombiaFil: Caminos, Jorge Eduardo. Universidad Nacional de Colombia; ColombiaMultidisciplinary Digital Publishing Institute2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218370Garcés, Maria Fernanda; Buell Acosta, Julieth Daniela; Ángel Müller, Edith; Parada Baños, Arturo José; Acosta Alvarez, Jaidy; et al.; Study of the ghrelin/LEAP-2 ratio in humans and rats during different phases of pregnancy; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 23; 17; 9-2022; 1-121661-65961422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/ijms23179514info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/17/9514info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:42:35Zoai:ri.conicet.gov.ar:11336/218370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:42:35.749CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
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15,811543 |