Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin
Fil: Clérico, G. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | Argentina |
| Institución: | Pontificia Universidad Católica Argentina |
| Repositorio: | Repositorio Institucional (UCA) |
| Idioma: | inglés |
| OAI Identifier: | oai:ucacris:123456789/10943 |
| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/10943 |
| Access Level: | acceso abierto |
| Palabra clave: | EQUINO OVOCITOS VITRIFICACION MELATONINA EMBARAZO |
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Argentina |
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| dc.title.none.fl_str_mv |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin |
| title |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin |
| spellingShingle |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin Clérico, Gabriel José EQUINO OVOCITOS VITRIFICACION MELATONINA EMBARAZO |
| title_short |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin |
| title_full |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin |
| title_fullStr |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin |
| title_full_unstemmed |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin |
| title_sort |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatonin |
| dc.creator.none.fl_str_mv |
Clérico, Gabriel José Taminelli, Guillermo Luis Veronesi, J. C. Polola, J. Pagura, N. Pinto, C. Sansiñena, Marina Julia |
| author |
Clérico, Gabriel José |
| author_facet |
Clérico, Gabriel José Taminelli, Guillermo Luis Veronesi, J. C. Polola, J. Pagura, N. Pinto, C. Sansiñena, Marina Julia |
| author_role |
author |
| author2 |
Taminelli, Guillermo Luis Veronesi, J. C. Polola, J. Pagura, N. Pinto, C. Sansiñena, Marina Julia |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
EQUINO OVOCITOS VITRIFICACION MELATONINA EMBARAZO |
| topic |
EQUINO OVOCITOS VITRIFICACION MELATONINA EMBARAZO |
| description |
Fil: Clérico, G. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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https://repositorio.uca.edu.ar/handle/123456789/10943 10.1016/j.theriogenology.2020.10.036 Clérico, G., Taminelli, G. , Veronesi, J.C , Polola, J., Pagura, N. , Pinto, C., Sansinena, M. Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin [en línea]. Theriogenology. 2021, (160). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10943 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/10943 |
| identifier_str_mv |
10.1016/j.theriogenology.2020.10.036 Clérico, G., Taminelli, G. , Veronesi, J.C , Polola, J., Pagura, N. , Pinto, C., Sansinena, M. Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin [en línea]. Theriogenology. 2021, (160). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10943 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| eu_rights_str_mv |
openAccess |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
Theriogenology vol.160, 2021 reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
| instname_str |
Pontificia Universidad Católica Argentina |
| reponame_str |
Repositorio Institucional (UCA) |
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Repositorio Institucional (UCA) |
| repository.name.fl_str_mv |
Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
| repository.mail.fl_str_mv |
claudia_fernandez@uca.edu.ar |
| _version_ |
1799197071941566464 |
| spelling |
Mitochondrial function, blastocyst development and live foals born after ICSI of immature 4 vitrified/warmed equine oocytes matured with or without melatoninClérico, Gabriel JoséTaminelli, Guillermo LuisVeronesi, J. C.Polola, J.Pagura, N.Pinto, C.Sansiñena, Marina JuliaEQUINOOVOCITOSVITRIFICACIONMELATONINAEMBARAZOFil: Clérico, G. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Taminelli, G. Pontificia Universidad Católica Argentina; ArgentinaFil: Veronesi, J. C. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Polola, J. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Pagura, N. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Pinto, C. Louisiana State School of Veterinary Medicine; Estados UnidosFil: Sansinena, M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sansinena, M. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Clérico, G. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sansinena, M. Louisiana State School of Veterinary Medicine; Estados UnidosAbstract: Oocyte vitrification is considered experimental in the horse with only three live foals reported. The oxidative conditions induced by vitrification could in part explain the poor results and melatonin, a powerful antioxidant, could stimulate ROS metabolization and restore mitochondrial function in these oocytes. Our objective was to determine the oxidative status of vitrified equine oocytes and to analyze the effect of melatonin on mitochondrial-specific ROS (mROS), oocyte maturation, ICSI embryo development and viability. Immature, abattoir-derived oocytes were held for 15 h and vitrified in a final concentration of 20% EG, 20% DMSO and 0.65 M trehalose. In Experiment 1, overall ROS was determined by DCHF-DA; vitrification increased ROS production compared to non-vitrified controls (1.29 ± 0.22 vs 0.74 ± 0.25 a. u.; P = 0.0156). In Experiment 2, mROS was analyzed by MitoSOX™ in vitrified/warmed oocytes matured with (+) or without (−) supplementation of 10−9 M melatonin; mROS decreased in vitrified and non-vitrified oocytes matured in presence of melatonin (P < 0.05). In Experiment 3, we assessed the effect of melatonin supplementation on oocyte maturation, embryo development after ICSI, and viability by pregnancy establishment. Melatonin did not improve oocyte maturation, cleavage or blastocyst rate of non-vitrified oocytes. However, vitrified melatonin (+) oocytes reached similar cleavage (61, 75 and 77%, respectively) and blastocyst rate (15, 29 and 26%, respectively) than non-vitrified, melatonin (+) and (−) oocytes. Vitrified, melatonin (−) oocytes had lower cleavage (46%) and blastocyst rate (9%) compared to non-vitrified groups (P < 0.05), but no significant differences were observed when compared to vitrified melatonin (+). Although the lack of available recipients precluded the transfer of every blastocyst produced in our study, transferred embryos from non-vitrified oocytes resulted in 50 and 83% pregnancy rates while embryos from vitrified oocytes resulted in 17 and 33% pregnancy rates, from melatonin (+) and (−) treatments respectively. Two healthy foals, one colt from melatonin (+) and one filly from melatonin (−) treatment, were born from vitrified/warmed oocytes. Gestation lengths (considering day 0 = day of ICSI) were 338 days for the colt and 329 days for the filly, respectively. Our work showed for the first time that in the horse, as in other species, intracellular reactive oxygen species are increased by the process of vitrification. Melatonin was useful in reducing mitochondrial-related ROS and improving ICSI embryo development, although the lower pregnancy rate in presence of melatonin should be further analyzed in future studies. To our knowledge this is the first report of melatonin supplementation to an in vitro embryo culture system and its use to improve embryo developmental competence of vitrified oocytes following ICSI.Elsevier2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/1094310.1016/j.theriogenology.2020.10.036Clérico, G., Taminelli, G. , Veronesi, J.C , Polola, J., Pagura, N. , Pinto, C., Sansinena, M. Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin [en línea]. Theriogenology. 2021, (160). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10943Theriogenology vol.160, 2021reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2024-05-15T12:24:48Zoai:ucacris:123456789/10943instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852024-05-15 12:24:49.107Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
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15,812429 |