Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes

The hemolytic uremic syndrome associated with diarrhea, a consequence of Shiga toxin (Stx)-producing Escherichia coli infection, is a common cause of pediatric acute renal failure in Argentina. Stx type 2a (Stx2a) causes direct damage to renal cells and induces local inflammatory responses that invo...

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Authors: Rosso, David Antonio, Rosato, Micaela, Gómez, Fernando Daniel, Alvarez, Romina Soledad, Shiromizu, Carolina Maiumi, Keitelman, Irene Angélica, Ibarra, Cristina Adriana, Amaral, María Marta, Jancic, Carolina Cristina
Format: article
Status:Published version
Publication Date:2021
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/172661
Online Access:http://hdl.handle.net/11336/172661
Access Level:Open access
Keyword:HEMOLYTIC UREMIC SYNDROME
INFLAMMATION
SHIGA TOXIN TYPE 2
TH1-LIKE PROFILE
ΓΔ T CELLS
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_ef58b860913a8a2a2ea2a72fd496a794
oai_identifier_str oai:ri.conicet.gov.ar:11336/172661
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
title Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
spellingShingle Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
Rosso, David Antonio
HEMOLYTIC UREMIC SYNDROME
INFLAMMATION
SHIGA TOXIN TYPE 2
TH1-LIKE PROFILE
ΓΔ T CELLS
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
title_full Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
title_fullStr Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
title_full_unstemmed Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
title_sort Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes
dc.creator.none.fl_str_mv Rosso, David Antonio
Rosato, Micaela
Gómez, Fernando Daniel
Alvarez, Romina Soledad
Shiromizu, Carolina Maiumi
Keitelman, Irene Angélica
Ibarra, Cristina Adriana
Amaral, María Marta
Jancic, Carolina Cristina
author Rosso, David Antonio
author_facet Rosso, David Antonio
Rosato, Micaela
Gómez, Fernando Daniel
Alvarez, Romina Soledad
Shiromizu, Carolina Maiumi
Keitelman, Irene Angélica
Ibarra, Cristina Adriana
Amaral, María Marta
Jancic, Carolina Cristina
author_role author
author2 Rosato, Micaela
Gómez, Fernando Daniel
Alvarez, Romina Soledad
Shiromizu, Carolina Maiumi
Keitelman, Irene Angélica
Ibarra, Cristina Adriana
Amaral, María Marta
Jancic, Carolina Cristina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HEMOLYTIC UREMIC SYNDROME
INFLAMMATION
SHIGA TOXIN TYPE 2
TH1-LIKE PROFILE
ΓΔ T CELLS
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic HEMOLYTIC UREMIC SYNDROME
INFLAMMATION
SHIGA TOXIN TYPE 2
TH1-LIKE PROFILE
ΓΔ T CELLS
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description The hemolytic uremic syndrome associated with diarrhea, a consequence of Shiga toxin (Stx)-producing Escherichia coli infection, is a common cause of pediatric acute renal failure in Argentina. Stx type 2a (Stx2a) causes direct damage to renal cells and induces local inflammatory responses that involve secretion of inflammatory mediators and the recruitment of innate immune cells. γδ T cells constitute a subset of T lymphocytes, which act as early sensors of cellular stress and infection. They can exert cytotoxicity against infected and transformed cells, and produce cytokines and chemokines. In this study, we investigated the activation of human peripheral γδ T cells in response to the incubation with Stx2a-stimulated human glomerular endothelial cells (HGEC) or their conditioned medium, by analyzing in γδ T lymphocytes, the expression of CD69, CD107a, and perforin, and the production of TNF-α and IFN-γ. In addition, we evaluated by confocal microscopy the contact between γδ T cells and HGEC. This analysis showed an augmentation in cellular interactions in the presence of Stx2a-stimulated HGEC compared to untreated HGEC. Furthermore, we observed an increase in cytokine production and CD107a expression, together with a decrease in intracellular perforin when γδ T cells were incubated with Stx2a-treated HGEC or their conditioned medium. Interestingly, the blocking of TNF-α by Etanercept reversed the changes in the parameters measured in γδ T cells incubated with Stx2a-treated HGEC supernatants. Altogether, our results suggest that soluble factors released by Stx2a-stimulated HGEC modulate the activation of γδ T cells, being TNF-α a key player during this process.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/172661
Rosso, David Antonio; Rosato, Micaela; Gómez, Fernando Daniel; Alvarez, Romina Soledad; Shiromizu, Carolina Maiumi; et al.; Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes; Frontiers Media S.A.; Frontiers in Cellular and Infection Microbiology; 11; 11-2021; 1-10
2235-2988
CONICET Digital
CONICET
url http://hdl.handle.net/11336/172661
identifier_str_mv Rosso, David Antonio; Rosato, Micaela; Gómez, Fernando Daniel; Alvarez, Romina Soledad; Shiromizu, Carolina Maiumi; et al.; Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes; Frontiers Media S.A.; Frontiers in Cellular and Infection Microbiology; 11; 11-2021; 1-10
2235-2988
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2021.765941
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T LymphocytesRosso, David AntonioRosato, MicaelaGómez, Fernando DanielAlvarez, Romina SoledadShiromizu, Carolina MaiumiKeitelman, Irene AngélicaIbarra, Cristina AdrianaAmaral, María MartaJancic, Carolina CristinaHEMOLYTIC UREMIC SYNDROMEINFLAMMATIONSHIGA TOXIN TYPE 2TH1-LIKE PROFILEΓΔ T CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The hemolytic uremic syndrome associated with diarrhea, a consequence of Shiga toxin (Stx)-producing Escherichia coli infection, is a common cause of pediatric acute renal failure in Argentina. Stx type 2a (Stx2a) causes direct damage to renal cells and induces local inflammatory responses that involve secretion of inflammatory mediators and the recruitment of innate immune cells. γδ T cells constitute a subset of T lymphocytes, which act as early sensors of cellular stress and infection. They can exert cytotoxicity against infected and transformed cells, and produce cytokines and chemokines. In this study, we investigated the activation of human peripheral γδ T cells in response to the incubation with Stx2a-stimulated human glomerular endothelial cells (HGEC) or their conditioned medium, by analyzing in γδ T lymphocytes, the expression of CD69, CD107a, and perforin, and the production of TNF-α and IFN-γ. In addition, we evaluated by confocal microscopy the contact between γδ T cells and HGEC. This analysis showed an augmentation in cellular interactions in the presence of Stx2a-stimulated HGEC compared to untreated HGEC. Furthermore, we observed an increase in cytokine production and CD107a expression, together with a decrease in intracellular perforin when γδ T cells were incubated with Stx2a-treated HGEC or their conditioned medium. Interestingly, the blocking of TNF-α by Etanercept reversed the changes in the parameters measured in γδ T cells incubated with Stx2a-treated HGEC supernatants. Altogether, our results suggest that soluble factors released by Stx2a-stimulated HGEC modulate the activation of γδ T cells, being TNF-α a key player during this process.Fil: Rosso, David Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Rosato, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Gómez, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Alvarez, Romina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Shiromizu, Carolina Maiumi. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Keitelman, Irene Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFrontiers Media S.A.2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/172661Rosso, David Antonio; Rosato, Micaela; Gómez, Fernando Daniel; Alvarez, Romina Soledad; Shiromizu, Carolina Maiumi; et al.; Human Glomerular Endothelial Cells Treated With Shiga Toxin Type 2 Activate γδ T Lymphocytes; Frontiers Media S.A.; Frontiers in Cellular and Infection Microbiology; 11; 11-2021; 1-102235-2988CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2021.765941info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:02:58Zoai:ri.conicet.gov.ar:11336/172661instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:02:59.216CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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