microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach

Background: microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. The particular developmental and metabolic characteristics of cestode...

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Detalhes bibliográficos
Autores: Macchiaroli, Natalia, Cucher, Marcela Alejandra, Zarowiecki, M., Maldonado, Lucas Luciano, Kamenetzky, Laura, Rosenzvit, Mara Cecilia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/113745
Acesso em linha:http://hdl.handle.net/11336/113745
Access Level:acceso abierto
Palavra-chave:MICRORNAS
HIGH-THROUGHPUT
ECHINOCOCCUS CANADENSIS
ECHINOCOCCOSIS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_edd1be52be21a9cf57ae722bd4ccf773
oai_identifier_str oai:ri.conicet.gov.ar:11336/113745
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
title microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
spellingShingle microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
Macchiaroli, Natalia
MICRORNAS
HIGH-THROUGHPUT
ECHINOCOCCUS CANADENSIS
ECHINOCOCCOSIS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
title_full microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
title_fullStr microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
title_full_unstemmed microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
title_sort microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach
dc.creator.none.fl_str_mv Macchiaroli, Natalia
Cucher, Marcela Alejandra
Zarowiecki, M.
Maldonado, Lucas Luciano
Kamenetzky, Laura
Rosenzvit, Mara Cecilia
author Macchiaroli, Natalia
author_facet Macchiaroli, Natalia
Cucher, Marcela Alejandra
Zarowiecki, M.
Maldonado, Lucas Luciano
Kamenetzky, Laura
Rosenzvit, Mara Cecilia
author_role author
author2 Cucher, Marcela Alejandra
Zarowiecki, M.
Maldonado, Lucas Luciano
Kamenetzky, Laura
Rosenzvit, Mara Cecilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MICRORNAS
HIGH-THROUGHPUT
ECHINOCOCCUS CANADENSIS
ECHINOCOCCOSIS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic MICRORNAS
HIGH-THROUGHPUT
ECHINOCOCCUS CANADENSIS
ECHINOCOCCOSIS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Background: microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. The particular developmental and metabolic characteristics of cestode parasites highlight the importance of studying miRNA gene regulation in these organisms. Here, we perform a comprehensive analysis of miRNAs in the parasitic cestode Echinococcus canadensis G7, one of the causative agents of the neglected zoonotic disease cystic echinococcosis. Methods: Small RNA libraries from protoscoleces and cyst walls of E. canadensis G7 and protoscoleces of E. granulosus sensu stricto G1 were sequenced using Illumina technology. For miRNA prediction, miRDeep2 core algorithm was used. The output list of candidate precursors was manually curated to generate a high confidence set of miRNAs. Differential expression analysis of miRNAs between stages or species was estimated with DESeq. Expression levels of selected miRNAs were validated using poly-A RT-qPCR. Results: In this study we used a high-throughput approach and found transcriptional evidence of 37 miRNAs thus expanding the miRNA repertoire of E. canadensis G7. Differential expression analysis showed highly regulated miRNAs between life cycle stages, suggesting a role in maintaining the features of each developmental stage or in the regulation of developmental timing. In this work we characterize conserved and novel Echinococcus miRNAs which represent 30 unique miRNA families. Here we confirmed the remarkable loss of conserved miRNA families in E. canadensis, reflecting their low morphological complexity and high adaptation to parasitism. Conclusions: We performed the first in-depth study profiling of small RNAs in the zoonotic parasite E. canadensis G7. We found that miRNAs are the preponderant small RNA silencing molecules, suggesting that these small RNAs could be an essential mechanism of gene regulation in this species. We also identified both parasite specific and divergent miRNAs which are potential biomarkers of infection. This study will provide valuable information for better understanding of the complex biology of this parasite and could help to find new potential targets for therapy and/or diagnosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113745
Macchiaroli, Natalia; Cucher, Marcela Alejandra; Zarowiecki, M.; Maldonado, Lucas Luciano; Kamenetzky, Laura; et al.; microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach; BioMed Central; Parasites and Vectors; 8; 83; 2-2015; 1-17
1756-3305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113745
identifier_str_mv Macchiaroli, Natalia; Cucher, Marcela Alejandra; Zarowiecki, M.; Maldonado, Lucas Luciano; Kamenetzky, Laura; et al.; microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach; BioMed Central; Parasites and Vectors; 8; 83; 2-2015; 1-17
1756-3305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-0686-8
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-015-0686-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195582313529344
spelling microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approachMacchiaroli, NataliaCucher, Marcela AlejandraZarowiecki, M.Maldonado, Lucas LucianoKamenetzky, LauraRosenzvit, Mara CeciliaMICRORNASHIGH-THROUGHPUTECHINOCOCCUS CANADENSISECHINOCOCCOSIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. The particular developmental and metabolic characteristics of cestode parasites highlight the importance of studying miRNA gene regulation in these organisms. Here, we perform a comprehensive analysis of miRNAs in the parasitic cestode Echinococcus canadensis G7, one of the causative agents of the neglected zoonotic disease cystic echinococcosis. Methods: Small RNA libraries from protoscoleces and cyst walls of E. canadensis G7 and protoscoleces of E. granulosus sensu stricto G1 were sequenced using Illumina technology. For miRNA prediction, miRDeep2 core algorithm was used. The output list of candidate precursors was manually curated to generate a high confidence set of miRNAs. Differential expression analysis of miRNAs between stages or species was estimated with DESeq. Expression levels of selected miRNAs were validated using poly-A RT-qPCR. Results: In this study we used a high-throughput approach and found transcriptional evidence of 37 miRNAs thus expanding the miRNA repertoire of E. canadensis G7. Differential expression analysis showed highly regulated miRNAs between life cycle stages, suggesting a role in maintaining the features of each developmental stage or in the regulation of developmental timing. In this work we characterize conserved and novel Echinococcus miRNAs which represent 30 unique miRNA families. Here we confirmed the remarkable loss of conserved miRNA families in E. canadensis, reflecting their low morphological complexity and high adaptation to parasitism. Conclusions: We performed the first in-depth study profiling of small RNAs in the zoonotic parasite E. canadensis G7. We found that miRNAs are the preponderant small RNA silencing molecules, suggesting that these small RNAs could be an essential mechanism of gene regulation in this species. We also identified both parasite specific and divergent miRNAs which are potential biomarkers of infection. This study will provide valuable information for better understanding of the complex biology of this parasite and could help to find new potential targets for therapy and/or diagnosis.Fil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Zarowiecki, M.. Wellcome Trust Sanger Institute; Reino UnidoFil: Maldonado, Lucas Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaBioMed Central2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113745Macchiaroli, Natalia; Cucher, Marcela Alejandra; Zarowiecki, M.; Maldonado, Lucas Luciano; Kamenetzky, Laura; et al.; microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach; BioMed Central; Parasites and Vectors; 8; 83; 2-2015; 1-171756-3305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-0686-8info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-015-0686-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:57:04Zoai:ri.conicet.gov.ar:11336/113745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:57:04.614CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15.812455

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