MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring reg...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2005 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/95126 |
| Online Access: | http://hdl.handle.net/11336/95126 |
| Access Level: | Open access |
| Keyword: | MCCJ25 AMIDATED DERIVATIVE MICROCIN J25 OXYGEN CONSUMPTION RNA POLYMERASE https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Summary: | Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the β-hairpin region and the C-terminal region is an important determinant for RNAP inhibition. |
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