Macrophage apoptosis using alendronate in targeted nanoarchaeosomes

Nanoarchaeosomes are non-hydrolysable nanovesicles made of archaeolipids, naturally functionalised with ligand for scavenger receptor class 1. We hypothesized that nitrogenate bisphosphonate alendronate (ALN) loaded nanoarchaeosomes (nanoarchaeosomes(ALN)) may constitute more efficient macrophage ta...

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Detalhes bibliográficos
Autores: Jerez, Horacio Emanuel, Altube, María Julia, Gándola, Yamila Belén, Gonzalez, Lorena, Gonzalez, Marina Cecilia, Morilla, María José, Romero, Eder Lilia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/164974
Acesso em linha:http://hdl.handle.net/11336/164974
Access Level:acceso abierto
Palavra-chave:APOPTOSIS
ARCHAEOLIPIDS
ENDOCYTOSIS
INFLAMMATION
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
id AR_ecd3df54fa4d47d6609f514ff5b294af
oai_identifier_str oai:ri.conicet.gov.ar:11336/164974
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
title Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
spellingShingle Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
Jerez, Horacio Emanuel
APOPTOSIS
ARCHAEOLIPIDS
ENDOCYTOSIS
INFLAMMATION
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
title_short Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
title_full Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
title_fullStr Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
title_full_unstemmed Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
title_sort Macrophage apoptosis using alendronate in targeted nanoarchaeosomes
dc.creator.none.fl_str_mv Jerez, Horacio Emanuel
Altube, María Julia
Gándola, Yamila Belén
Gonzalez, Lorena
Gonzalez, Marina Cecilia
Morilla, María José
Romero, Eder Lilia
author Jerez, Horacio Emanuel
author_facet Jerez, Horacio Emanuel
Altube, María Julia
Gándola, Yamila Belén
Gonzalez, Lorena
Gonzalez, Marina Cecilia
Morilla, María José
Romero, Eder Lilia
author_role author
author2 Altube, María Julia
Gándola, Yamila Belén
Gonzalez, Lorena
Gonzalez, Marina Cecilia
Morilla, María José
Romero, Eder Lilia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
ARCHAEOLIPIDS
ENDOCYTOSIS
INFLAMMATION
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
topic APOPTOSIS
ARCHAEOLIPIDS
ENDOCYTOSIS
INFLAMMATION
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
description Nanoarchaeosomes are non-hydrolysable nanovesicles made of archaeolipids, naturally functionalised with ligand for scavenger receptor class 1. We hypothesized that nitrogenate bisphosphonate alendronate (ALN) loaded nanoarchaeosomes (nanoarchaeosomes(ALN)) may constitute more efficient macrophage targeted apoptotic inducers than ALN loaded nanoliposomes (nanoliposomes (ALN)). To that aim, ALN was loaded in cholesterol containing (nanoARC-chol(ALN)) or not (nanoARC(ALN)) nanoarchaeosomes. Nanoarchaeosomes(ALN) (220–320 nm sized, ~ −40 mV ξ potential, 38–50 μg ALN/mg lipid ratio) displayed higher structural stability than nanoliposomes(ALN) of matching size and ξ potential, retaining most of ALN against a 1/200 folds dilution. The cytotoxicity of nanoARC(ALN) on J774A.1 cells, resulted > 30 folds higher than free ALN and nanoliposomes(ALN) and was reduced by cholesterol in nanoARC-chol(ALN). Devoid of ALN, nanoARC-chol was non-cytotoxic, exhibited pronounced anti-inflammatory activity on J774.1 cells, strongly reducing reactive oxygen species (ROS) and IL-6 induced by LPS. Nanoarchaeosomes bilayer extensively interacted with serum proteins but resulted refractory to phospholipases. Upon J774A.1 cells uptake, nanoarchaeosomes induced cytoplasmic acid vesicles, reduced the mitochondrial membrane potential by 20–40 % without consuming ATP neither damaging lysosomes and increasing pERK. Refractory to chemoenzymatic attacks, either void or drug loaded, nanoarchaeosomes induced either anti-inflammation or macrophages apoptosis, constituting promising targeted nanovesicles for multiple therapeutic purposes.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164974
Jerez, Horacio Emanuel; Altube, María Julia; Gándola, Yamila Belén; Gonzalez, Lorena; Gonzalez, Marina Cecilia; et al.; Macrophage apoptosis using alendronate in targeted nanoarchaeosomes; Elsevier Science; European Journal of Pharmaceutics and Biopharmaceutics; 160; 3-2021; 42-54
0939-6411
1873-3441
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164974
identifier_str_mv Jerez, Horacio Emanuel; Altube, María Julia; Gándola, Yamila Belén; Gonzalez, Lorena; Gonzalez, Marina Cecilia; et al.; Macrophage apoptosis using alendronate in targeted nanoarchaeosomes; Elsevier Science; European Journal of Pharmaceutics and Biopharmaceutics; 160; 3-2021; 42-54
0939-6411
1873-3441
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0939641121000011
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejpb.2021.01.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194766819196928
spelling Macrophage apoptosis using alendronate in targeted nanoarchaeosomesJerez, Horacio EmanuelAltube, María JuliaGándola, Yamila BelénGonzalez, LorenaGonzalez, Marina CeciliaMorilla, María JoséRomero, Eder LiliaAPOPTOSISARCHAEOLIPIDSENDOCYTOSISINFLAMMATIONhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Nanoarchaeosomes are non-hydrolysable nanovesicles made of archaeolipids, naturally functionalised with ligand for scavenger receptor class 1. We hypothesized that nitrogenate bisphosphonate alendronate (ALN) loaded nanoarchaeosomes (nanoarchaeosomes(ALN)) may constitute more efficient macrophage targeted apoptotic inducers than ALN loaded nanoliposomes (nanoliposomes (ALN)). To that aim, ALN was loaded in cholesterol containing (nanoARC-chol(ALN)) or not (nanoARC(ALN)) nanoarchaeosomes. Nanoarchaeosomes(ALN) (220–320 nm sized, ~ −40 mV ξ potential, 38–50 μg ALN/mg lipid ratio) displayed higher structural stability than nanoliposomes(ALN) of matching size and ξ potential, retaining most of ALN against a 1/200 folds dilution. The cytotoxicity of nanoARC(ALN) on J774A.1 cells, resulted > 30 folds higher than free ALN and nanoliposomes(ALN) and was reduced by cholesterol in nanoARC-chol(ALN). Devoid of ALN, nanoARC-chol was non-cytotoxic, exhibited pronounced anti-inflammatory activity on J774.1 cells, strongly reducing reactive oxygen species (ROS) and IL-6 induced by LPS. Nanoarchaeosomes bilayer extensively interacted with serum proteins but resulted refractory to phospholipases. Upon J774A.1 cells uptake, nanoarchaeosomes induced cytoplasmic acid vesicles, reduced the mitochondrial membrane potential by 20–40 % without consuming ATP neither damaging lysosomes and increasing pERK. Refractory to chemoenzymatic attacks, either void or drug loaded, nanoarchaeosomes induced either anti-inflammation or macrophages apoptosis, constituting promising targeted nanovesicles for multiple therapeutic purposes.Fil: Jerez, Horacio Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; ArgentinaFil: Altube, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; ArgentinaFil: Gándola, Yamila Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Gonzalez, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Gonzalez, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Morilla, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; ArgentinaFil: Romero, Eder Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Diseño de Estrategias de Targeting de Drogas; ArgentinaElsevier Science2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/164974Jerez, Horacio Emanuel; Altube, María Julia; Gándola, Yamila Belén; Gonzalez, Lorena; Gonzalez, Marina Cecilia; et al.; Macrophage apoptosis using alendronate in targeted nanoarchaeosomes; Elsevier Science; European Journal of Pharmaceutics and Biopharmaceutics; 160; 3-2021; 42-540939-64111873-3441CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0939641121000011info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejpb.2021.01.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:35:56Zoai:ri.conicet.gov.ar:11336/164974instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:35:57.041CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15.812429

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