Modulation of innate immunity in chickens induced by in vivo administration of baculovirus

Baculoviruses stimulate cytokine production in mammalian cells. They induce a strong innate immune response in animals and have adjuvant properties. The purpose of this work was to study the in vivo effect of baculovirus on chicken innate immune response. SPF chickens were inoculated intravenously w...

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Detalhes bibliográficos
Autores: Chimeno Zoth, Silvina Andrea, Carballeda, Juan Manuel, Gómez, Evangelina, Gravisaco, María José, Carrillo, Elisa Cristina, Berinstein, Analía
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2012
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/194502
Acesso em linha:http://hdl.handle.net/11336/194502
Access Level:Acceso aberto
Palavra-chave:AVIAN INNATE IMMUNITY
BACULOVIRUS
FLOW CYTOMETRY
REAL TIME PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194502
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
title Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
spellingShingle Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
Chimeno Zoth, Silvina Andrea
AVIAN INNATE IMMUNITY
BACULOVIRUS
FLOW CYTOMETRY
REAL TIME PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
title_full Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
title_fullStr Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
title_full_unstemmed Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
title_sort Modulation of innate immunity in chickens induced by in vivo administration of baculovirus
dc.creator.none.fl_str_mv Chimeno Zoth, Silvina Andrea
Carballeda, Juan Manuel
Gómez, Evangelina
Gravisaco, María José
Carrillo, Elisa Cristina
Berinstein, Analía
author Chimeno Zoth, Silvina Andrea
author_facet Chimeno Zoth, Silvina Andrea
Carballeda, Juan Manuel
Gómez, Evangelina
Gravisaco, María José
Carrillo, Elisa Cristina
Berinstein, Analía
author_role author
author2 Carballeda, Juan Manuel
Gómez, Evangelina
Gravisaco, María José
Carrillo, Elisa Cristina
Berinstein, Analía
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AVIAN INNATE IMMUNITY
BACULOVIRUS
FLOW CYTOMETRY
REAL TIME PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic AVIAN INNATE IMMUNITY
BACULOVIRUS
FLOW CYTOMETRY
REAL TIME PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Baculoviruses stimulate cytokine production in mammalian cells. They induce a strong innate immune response in animals and have adjuvant properties. The purpose of this work was to study the in vivo effect of baculovirus on chicken innate immune response. SPF chickens were inoculated intravenously with Autographa californica nuclear polyhedrosis virus (BV). Three hours later, chickens were bled, euthanized and their spleen, duodenum and cecal tonsils were excised in order to take samples for RNA extraction and real time PCR, and to isolate lymphocytes, which were stained and analyzed by flow cytometry. The results obtained showed that baculovirus inoculation up-regulates the expression ofIFNg, IL-6 and LITAF in spleen cells. This result (IFNg) correlated with that obtained by ELISA which showed a very strong increase of IFNg in chicken plasma. Flow cytometry analysis revealed that BV inoculation induced in spleen an increase in the percentage of monocyte/macrophage population together with an increase in CD3+CD4+T lymphocytes. On the other hand, BV inoculation decreased the percentage of CD3+CD4+T lymphocytes and increased the percentage of NK cells in cecal tonsils. However, intraepithelial lymphocytes of the gut did not show differences between BV and control treated animals. Even though further studies in order to understand the mechanisms by which BVs affect the avian immune response are needed, results obtained in the present work demonstrate the ability of BVs to stimulate the innate immunity in chickens, modifying the expression pattern of related genes and the profile of the immune cells involved.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194502
Chimeno Zoth, Silvina Andrea; Carballeda, Juan Manuel; Gómez, Evangelina; Gravisaco, María José; Carrillo, Elisa Cristina; et al.; Modulation of innate immunity in chickens induced by in vivo administration of baculovirus; Elsevier Science; Veterinary Immunology And Immunopathology; 145; 2; 1-2012; 241-247
0165-2427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194502
identifier_str_mv Chimeno Zoth, Silvina Andrea; Carballeda, Juan Manuel; Gómez, Evangelina; Gravisaco, María José; Carrillo, Elisa Cristina; et al.; Modulation of innate immunity in chickens induced by in vivo administration of baculovirus; Elsevier Science; Veterinary Immunology And Immunopathology; 145; 2; 1-2012; 241-247
0165-2427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0165242711004533
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2011.11.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195089328668672
spelling Modulation of innate immunity in chickens induced by in vivo administration of baculovirusChimeno Zoth, Silvina AndreaCarballeda, Juan ManuelGómez, EvangelinaGravisaco, María JoséCarrillo, Elisa CristinaBerinstein, AnalíaAVIAN INNATE IMMUNITYBACULOVIRUSFLOW CYTOMETRYREAL TIME PCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Baculoviruses stimulate cytokine production in mammalian cells. They induce a strong innate immune response in animals and have adjuvant properties. The purpose of this work was to study the in vivo effect of baculovirus on chicken innate immune response. SPF chickens were inoculated intravenously with Autographa californica nuclear polyhedrosis virus (BV). Three hours later, chickens were bled, euthanized and their spleen, duodenum and cecal tonsils were excised in order to take samples for RNA extraction and real time PCR, and to isolate lymphocytes, which were stained and analyzed by flow cytometry. The results obtained showed that baculovirus inoculation up-regulates the expression ofIFNg, IL-6 and LITAF in spleen cells. This result (IFNg) correlated with that obtained by ELISA which showed a very strong increase of IFNg in chicken plasma. Flow cytometry analysis revealed that BV inoculation induced in spleen an increase in the percentage of monocyte/macrophage population together with an increase in CD3+CD4+T lymphocytes. On the other hand, BV inoculation decreased the percentage of CD3+CD4+T lymphocytes and increased the percentage of NK cells in cecal tonsils. However, intraepithelial lymphocytes of the gut did not show differences between BV and control treated animals. Even though further studies in order to understand the mechanisms by which BVs affect the avian immune response are needed, results obtained in the present work demonstrate the ability of BVs to stimulate the innate immunity in chickens, modifying the expression pattern of related genes and the profile of the immune cells involved.Fil: Chimeno Zoth, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Carballeda, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Gómez, Evangelina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Berinstein, Analía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194502Chimeno Zoth, Silvina Andrea; Carballeda, Juan Manuel; Gómez, Evangelina; Gravisaco, María José; Carrillo, Elisa Cristina; et al.; Modulation of innate immunity in chickens induced by in vivo administration of baculovirus; Elsevier Science; Veterinary Immunology And Immunopathology; 145; 2; 1-2012; 241-2470165-2427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0165242711004533info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2011.11.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:43:14Zoai:ri.conicet.gov.ar:11336/194502instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:43:15.145CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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