Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period

The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period...

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Autores: Pellegrino, Matias Santiago, Berardo, Natanael, Giraudo, Jose Angel, Nader, Maria Elena Fatima, Bogni, Cristina Ines
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/82632
Acesso em linha:http://hdl.handle.net/11336/82632
Access Level:acceso abierto
Palavra-chave:Bovine Mastitis
Lactic Acid Bacteria
Lactobacillus
Probiotics
Staphylococcus
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
id AR_ec306e7c7f2ec830f1bff7bd7eef6bc2
oai_identifier_str oai:ri.conicet.gov.ar:11336/82632
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
title Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
spellingShingle Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
Pellegrino, Matias Santiago
Bovine Mastitis
Lactic Acid Bacteria
Lactobacillus
Probiotics
Staphylococcus
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
title_short Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
title_full Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
title_fullStr Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
title_full_unstemmed Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
title_sort Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period
dc.creator.none.fl_str_mv Pellegrino, Matias Santiago
Berardo, Natanael
Giraudo, Jose Angel
Nader, Maria Elena Fatima
Bogni, Cristina Ines
author Pellegrino, Matias Santiago
author_facet Pellegrino, Matias Santiago
Berardo, Natanael
Giraudo, Jose Angel
Nader, Maria Elena Fatima
Bogni, Cristina Ines
author_role author
author2 Berardo, Natanael
Giraudo, Jose Angel
Nader, Maria Elena Fatima
Bogni, Cristina Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bovine Mastitis
Lactic Acid Bacteria
Lactobacillus
Probiotics
Staphylococcus
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
topic Bovine Mastitis
Lactic Acid Bacteria
Lactobacillus
Probiotics
Staphylococcus
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
description The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied. Ten animals were inoculated intramammarily with 106 cells of each LAB (IG) and 2 animals used as control (NIG). Milk and blood samples were taken before inoculation and 1, 2, 4, 6, 12 and 24 h and 7 and 14 days after inoculation. Somatic cell count (SCC) in milk, the presence of bovine mastitis pathogens, the levels of antibodies and lymphocyte proliferation were determined. In the IG, the SCC was < 250,000 cells/ml up to 4 h after intramammary inoculation. Six and 12 h after inoculation, the SCC increased up to 600,000 and 2,000,000 cells/ml, respectively. In the NIG, the SCC reached the maximum value 7 days after inoculation. Microbiological analysis showed that all samples were negative for major bovine mastitis pathogens after 24-48 h of incubation. In general, LAB inoculation increased the amount of IgG isotypes in blood and milk, and these antibodies were able to recognise Staphylococcus aureus epitopes. Lymphocytes proliferation was significantly higher in the IG at all time points assayed, following LAB or S. aureus stimulation. The lymphocytes of animals inoculated with LAB do not react in vitro to the presence of S. aureus antigen.. The results showed that probiotic microorganisms could be a natural and effective alternative in the prevention of bovine mastitis at dry-off period and act as immunomodulatory stimulating local and systemic defence lines.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82632
Pellegrino, Matias Santiago; Berardo, Natanael; Giraudo, Jose Angel; Nader, Maria Elena Fatima; Bogni, Cristina Ines; Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period; Wageningen Academic Publishers; Beneficial Microbes; 8; 4; 13-7-2017; 589-596
1876-2883
1876-2891
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82632
identifier_str_mv Pellegrino, Matias Santiago; Berardo, Natanael; Giraudo, Jose Angel; Nader, Maria Elena Fatima; Bogni, Cristina Ines; Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period; Wageningen Academic Publishers; Beneficial Microbes; 8; 4; 13-7-2017; 589-596
1876-2883
1876-2891
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3920/BM2016.0194
info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.3920/BM2016.0194
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic Publishers
publisher.none.fl_str_mv Wageningen Academic Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195107613736960
spelling Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off periodPellegrino, Matias SantiagoBerardo, NatanaelGiraudo, Jose AngelNader, Maria Elena FatimaBogni, Cristina InesBovine MastitisLactic Acid BacteriaLactobacillusProbioticsStaphylococcushttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied. Ten animals were inoculated intramammarily with 106 cells of each LAB (IG) and 2 animals used as control (NIG). Milk and blood samples were taken before inoculation and 1, 2, 4, 6, 12 and 24 h and 7 and 14 days after inoculation. Somatic cell count (SCC) in milk, the presence of bovine mastitis pathogens, the levels of antibodies and lymphocyte proliferation were determined. In the IG, the SCC was < 250,000 cells/ml up to 4 h after intramammary inoculation. Six and 12 h after inoculation, the SCC increased up to 600,000 and 2,000,000 cells/ml, respectively. In the NIG, the SCC reached the maximum value 7 days after inoculation. Microbiological analysis showed that all samples were negative for major bovine mastitis pathogens after 24-48 h of incubation. In general, LAB inoculation increased the amount of IgG isotypes in blood and milk, and these antibodies were able to recognise Staphylococcus aureus epitopes. Lymphocytes proliferation was significantly higher in the IG at all time points assayed, following LAB or S. aureus stimulation. The lymphocytes of animals inoculated with LAB do not react in vitro to the presence of S. aureus antigen.. The results showed that probiotic microorganisms could be a natural and effective alternative in the prevention of bovine mastitis at dry-off period and act as immunomodulatory stimulating local and systemic defence lines.Fil: Pellegrino, Matias Santiago. Universidad Nacional de Río Cuarto; ArgentinaFil: Berardo, Natanael. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giraudo, Jose Angel. Universidad Nacional de Río Cuarto; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Bogni, Cristina Ines. Universidad Nacional de Río Cuarto; ArgentinaWageningen Academic Publishers2017-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82632Pellegrino, Matias Santiago; Berardo, Natanael; Giraudo, Jose Angel; Nader, Maria Elena Fatima; Bogni, Cristina Ines; Bovine mastitis prevention: Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period; Wageningen Academic Publishers; Beneficial Microbes; 8; 4; 13-7-2017; 589-5961876-28831876-2891CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3920/BM2016.0194info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.3920/BM2016.0194info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:43:40Zoai:ri.conicet.gov.ar:11336/82632instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:43:40.395CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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