Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)

Fil: Ben Guerrero, Emiliano.Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina.

Detalles Bibliográficos
Autores: Ben Guerrero, Emiliano, Soria, Marcelo Abel, Salvador, Ricardo, Ceja Navarro, Javier A., Campos, Eleonora, Brodie, Eoin L., Talia, Paola
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Institución:Universidad de Buenos Aires. Facultad de Agronomía
Repositorio:FAUBA Digital (UBA-FAUBA)
Idioma:inglés
OAI Identifier:snrd:2016guerrero
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016guerrero
Access Level:acceso abierto
Palabra clave:ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
id AR_eb1e1a98330c3b28b3aa054eac57064d
oai_identifier_str snrd:2016guerrero
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
spellingShingle Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
Ben Guerrero, Emiliano
ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
title_short Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_full Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_fullStr Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_full_unstemmed Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
title_sort Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)
dc.creator.none.fl_str_mv Ben Guerrero, Emiliano
Soria, Marcelo Abel
Salvador, Ricardo
Ceja Navarro, Javier A.
Campos, Eleonora
Brodie, Eoin L.
Talia, Paola
author Ben Guerrero, Emiliano
author_facet Ben Guerrero, Emiliano
Soria, Marcelo Abel
Salvador, Ricardo
Ceja Navarro, Javier A.
Campos, Eleonora
Brodie, Eoin L.
Talia, Paola
author_role author
author2 Soria, Marcelo Abel
Salvador, Ricardo
Ceja Navarro, Javier A.
Campos, Eleonora
Brodie, Eoin L.
Talia, Paola
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
topic ANTHONOMUS GRANDIS
GUT MICROBIOTA
16S RRNA GENE
ILLUMINA AMPLICON SEQUENCING
HYDROLYTIC ACTIVITIES
LIGNOCELLULOSIC FEEDSTOCKS
description Fil: Ben Guerrero, Emiliano.Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 10.3389/fmicb.2016.02093
1664-302X
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016guerrero
identifier_str_mv 10.3389/fmicb.2016.02093
1664-302X
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016guerrero
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Frontiers in Microbiology
Vol.7
e2093,123p.
https://www.frontiersin.org/
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
instname_str Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
_version_ 1799196712182480896
spelling Effect of different lignocellulosic diets on bacterial microbiota and hydrolytic enzyme activities in the gut of the cotton boll weevil (Anthonomus grandis)Ben Guerrero, EmilianoSoria, Marcelo AbelSalvador, RicardoCeja Navarro, Javier A.Campos, EleonoraBrodie, Eoin L.Talia, PaolaANTHONOMUS GRANDISGUT MICROBIOTA16S RRNA GENEILLUMINA AMPLICON SEQUENCINGHYDROLYTIC ACTIVITIESLIGNOCELLULOSIC FEEDSTOCKSFil: Ben Guerrero, Emiliano.Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Centro Nacional de Investigaciones Agropecuarias. Instituto de Investigación Microbiología y Zoología Agrícola (IMyZA). Hurlingham, Buenos Aires, Argentina.Fil: Ceja Navarro, Javier A. Earth and Environmental Sciences. Lawrence Berkeley National Laboratory, Berkeley, CA, USA.Fil: Campos, Eleonora. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina.Fil: Brodie, Eoin L. Sciences. Lawrence Berkeley National Laboratory, Berkeley, CA, USA.Fil: Talia, Paola. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología. Buenos Aires, Argentina.Cotton boll weevils, Anthonomus grandis, are omnivorous coleopteran that can feed on diets with different compositions, including recalcitrant lignocellulosic materials. We characterized the changes in the prokaryotic community structure and the hydrolytic activities of A. grandis larvae fed on different lignocellulosic diets. A. grandis larvae were fed on three different artificial diets: cottonseed meal (CM), Napier grass (NG) and corn stover (CS). Total DNA was extracted from the gut samples for amplification and sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Proteobacteria and Firmicutes dominated the gut microbiota followed by Actinobacteria, Spirochaetes and a small number of unclassified phyla in CM and NG microbiomes. In the CS feeding group, members of Spirochaetes were the most prevalent, followed by Proteobacteria and Firmicutes. Bray–Curtis distances showed that the samples from the CS community were clearly separated from those samples of the CM and NG diets. Gut extracts from all three diets exhibited endoglucanase, xylanase, ß-glucosidase and pectinase activities. These activities were significantly affected by pH and temperature across different diets. We observed that the larvae reared on a CM showed significantly higher activities than larvae reared on NG and CS. We demonstrated that the intestinal bacterial community structure varies depending on diet composition. Diets with more variable and complex compositions, such as CS, showed higher bacterial diversity and richness than the two other diets. In spite of the detected changes in composition and diversity, we identified a core microbiome shared between the three different lignocellulosic diets. These results suggest that feeding with diets of different lignocellulosic composition could be a viable strategy to discover variants of hemicellulose and cellulose breakdown systems.grafs., tbls.2016info:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf10.3389/fmicb.2016.020931664-302Xhttp://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2016guerreroFrontiers in MicrobiologyVol.7e2093,123p.https://www.frontiersin.org/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42024-05-10T10:43:21Zsnrd:2016guerreroinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292024-05-10 10:43:22.037FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
score 15,812455

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