Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera

Fil: Mejias, María Pilar. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas-Academia Nacional de Medicina, Buenos Aires; Argentina.

Detalles Bibliográficos
Autores: Mejías, M P, Cabrera, Gabriel, Fernandez-Brando, Romina J, Baschkier, Ariela, Ghersi, Giselle, Abrey-Recalde, M J, Miliwebsky, Elizabeth, Meiss, R, Goldbaum, Fernando A, Zylberman, Vanesa, Rivas, Marta, Palermo, M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Argentina
Institución:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
Repositorio:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Idioma:inglés
OAI Identifier:oai:sgc.anlis.gob.ar:Publications/123456789/2328
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993387/
http://sgc.anlis.gob.ar/handle/123456789/2328
Access Level:acceso abierto
Palabra clave:Toxina Shiga
Brucella
Escherichia coli
id AR_ea5a5a206306dff60b5b324e25f6eb8a
oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/2328
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
title Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
spellingShingle Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
Mejías, M P
Toxina Shiga
Brucella
Escherichia coli
title_short Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
title_full Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
title_fullStr Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
title_full_unstemmed Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
title_sort Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera
dc.creator.none.fl_str_mv Mejías, M P
Cabrera, Gabriel
Fernandez-Brando, Romina J
Baschkier, Ariela
Ghersi, Giselle
Abrey-Recalde, M J
Miliwebsky, Elizabeth
Meiss, R
Goldbaum, Fernando A
Zylberman, Vanesa
Rivas, Marta
Palermo, M.
author Mejías, M P
author_facet Mejías, M P
Cabrera, Gabriel
Fernandez-Brando, Romina J
Baschkier, Ariela
Ghersi, Giselle
Abrey-Recalde, M J
Miliwebsky, Elizabeth
Meiss, R
Goldbaum, Fernando A
Zylberman, Vanesa
Rivas, Marta
Palermo, M.
author_role author
author2 Cabrera, Gabriel
Fernandez-Brando, Romina J
Baschkier, Ariela
Ghersi, Giselle
Abrey-Recalde, M J
Miliwebsky, Elizabeth
Meiss, R
Goldbaum, Fernando A
Zylberman, Vanesa
Rivas, Marta
Palermo, M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Toxina Shiga
Brucella
Escherichia coli
topic Toxina Shiga
Brucella
Escherichia coli
description Fil: Mejias, María Pilar. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas-Academia Nacional de Medicina, Buenos Aires; Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1098-5522
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993387/
http://sgc.anlis.gob.ar/handle/123456789/2328
10.1128/IAI.00027-14
identifier_str_mv 1098-5522
10.1128/IAI.00027-14
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993387/
http://sgc.anlis.gob.ar/handle/123456789/2328
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Infection and immunity
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Infection and Immunity 2014; 82(4):1491-9.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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spelling Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimeraMejías, M PCabrera, GabrielFernandez-Brando, Romina JBaschkier, ArielaGhersi, GiselleAbrey-Recalde, M JMiliwebsky, ElizabethMeiss, RGoldbaum, Fernando AZylberman, VanesaRivas, MartaPalermo, M.Toxina ShigaBrucellaEscherichia coliFil: Mejias, María Pilar. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas-Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Cabrera, Gabriel. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas-Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Fernández-Brando, Romina Jimena. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas-Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Ghersi, Giselle. Inmunova S.A., Buenos Aires; ArgentinaFil: Abrey-Recalde, Maria Jimena. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas-Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Meiss, Roberto. Departamento de Patología, Centro de Estudios Oncológicos, Academia Nacional de Medicina, Buenos Aires; Argentina.Fil: Goldbaum, Fernando. Inmunova S.A., Buenos Aires; ArgentinaFil: Zylberman, Vanesa. Inmunova S.A., Buenos Aires; Argentina.Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Palermo, Marina Sandra. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas-Academia Nacional de Medicina, Buenos Aires; Argentina.Hemolytic-uremic syndrome (HUS) is defined as the triad of anemia, thrombocytopenia, and acute kidney injury. Enterohemorrhagic Shiga toxin (Stx)-producing Escherichia coli (EHEC), which causes a prodromal hemorrhagic enteritis, remains the most common etiology of the typical or epidemic form of HUS. Because no licensed vaccine or effective therapy is presently available for human use, we recently developed a novel immunogen based on the B subunit of Shiga toxin 2 (Stx2B) and the enzyme lumazine synthase from Brucella spp. (BLS) (BLS-Stx2B). The aim of this study was to analyze maternal immunization with BLS-Stx2B as a possible approach for transferring anti-Stx2 protection to the offspring. BALB/c female mice were immunized with BLS-Stx2B before mating. Both dams and pups presented comparable titers of anti-Stx2B antibodies in sera and fecal extracts. Moreover, pups were totally protected against a lethal dose of systemic Stx2 injection up to 2 to 3 months postpartum. In addition, pups were resistant to an oral challenge with an Stx2-producing EHEC strain at weaning and did not develop any symptomatology associated with Stx2 toxicity. Fostering experiments demonstrated that anti-Stx2B neutralizing IgG antibodies were transmitted through breast-feeding. Pups that survived the EHEC infection due to maternally transferred immunity prolonged an active and specific immune response that protected them against a subsequent challenge with intravenous Stx2. Our study shows that maternal immunization with BLS-Stx2B was very effective at promoting the transfer of specific antibodies, and suggests that preexposure of adult females to this immunogen could protect their offspring during the early phase of life.American Society for Microbiology2014-04info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-5522https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993387/http://sgc.anlis.gob.ar/handle/123456789/232810.1128/IAI.00027-14Infection and Immunity 2014; 82(4):1491-9.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISInfection and immunityenginfo:eu-repo/semantics/openAccess2024-05-10T11:52:29Zoai:sgc.anlis.gob.ar:Publications/123456789/2328Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2024-05-10 11:52:30.13Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
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