Heteroalleles in Common Wheat
The Gli-B1-encoded -gliadins and non-coding -gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major -gliadin, restriction fragment length polymorphism patterns (RFLPs) (three di...
| Authors: | , , , , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2021 |
| Country: | Argentina |
| Institution: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repository: | CIC Digital (CICBA) |
| Language: | English |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/11964 |
| Online Access: | https://digital.cic.gba.gob.ar/handle/11746/11964 |
| Access Level: | Open access |
| Keyword: | Ciencias Biológicas Triticum aestivum Gli-B1 γ-gliadin polymorphism APAGE PCR DNA sequencing |
| Summary: | The Gli-B1-encoded -gliadins and non-coding -gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major -gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), Gli-B1- -gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene GAG56B. It was discovered that encoded -gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles Gli-B1i, k, m, o), II (Gli-B1n, q, s), III (Gli-B1b), IV (Gli-B1e, f, g), V (Gli-B1h), VI (Gli-B1d) and VII (Gli-B1a). Allele Gli-B1c (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the Gli-B1 locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called “heteroallelic”. The donor’s particularities at the Gli-B1 locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat. |
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