Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis

The apoplast, i.e. the cellular compartment external to the plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar to the apoplast alkalizati...

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Detalhes bibliográficos
Autores: Borniego, María Lucía, Steelheart Molina, Maria Charlotte, Guiamet, Juan José, Martinez, Dana Ethel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/180822
Acesso em linha:http://hdl.handle.net/11336/180822
Access Level:acceso abierto
Palavra-chave:APOPLAST
APOPLASTIC FLUID
EXTRACELLULAR PH
PATHOGENESIS-RELATED PROTEIN
PR
SECRETOME
SENESCENCE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
spellingShingle Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
Borniego, María Lucía
APOPLAST
APOPLASTIC FLUID
EXTRACELLULAR PH
PATHOGENESIS-RELATED PROTEIN
PR
SECRETOME
SENESCENCE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_full Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_fullStr Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_full_unstemmed Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
title_sort Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis
dc.creator.none.fl_str_mv Borniego, María Lucía
Steelheart Molina, Maria Charlotte
Guiamet, Juan José
Martinez, Dana Ethel
author Borniego, María Lucía
author_facet Borniego, María Lucía
Steelheart Molina, Maria Charlotte
Guiamet, Juan José
Martinez, Dana Ethel
author_role author
author2 Steelheart Molina, Maria Charlotte
Guiamet, Juan José
Martinez, Dana Ethel
author2_role author
author
author
dc.subject.none.fl_str_mv APOPLAST
APOPLASTIC FLUID
EXTRACELLULAR PH
PATHOGENESIS-RELATED PROTEIN
PR
SECRETOME
SENESCENCE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic APOPLAST
APOPLASTIC FLUID
EXTRACELLULAR PH
PATHOGENESIS-RELATED PROTEIN
PR
SECRETOME
SENESCENCE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The apoplast, i.e. the cellular compartment external to the plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar to the apoplast alkalization in response to abiotic stresses. The levels of 159 proteins decrease, whereas 24 proteins increase in relative abundance in the apoplast of senescing leaves. Around half of the apoplastic proteins of non-senescent leaves contain a N-terminal signal peptide for secretion, while all the identified senescence-associated apoplastic proteins contain the signal peptide. Several of the apoplastic proteins that accumulate during senescence also accumulate in stress responses, suggesting that the apoplast may constitute a compartment where developmental and stress-related programs overlap. Other senescence-related apoplastic proteins are involved in cell wall modifications, proteolysis, carbohydrate, ROS and amino acid metabolism, signaling, lipid transport, etc. The most abundant senescence-associated apoplastic proteins, PR2 and PR5 (e.g. pathogenesis related proteins PR2 and PR5) are related to leaf aging rather than to the chloroplast degradation program, as their levels increase only in leaves undergoing developmental senescence, but not in dark-induced senescent leaves. Changes in the apoplastic space may be relevant for signaling and molecular trafficking underlying senescence.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180822
Borniego, María Lucía; Steelheart Molina, Maria Charlotte; Guiamet, Juan José; Martinez, Dana Ethel; Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis; Frontiers Media; Frontiers in Plant Science; 10; 1-2020; 1-20
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180822
identifier_str_mv Borniego, María Lucía; Steelheart Molina, Maria Charlotte; Guiamet, Juan José; Martinez, Dana Ethel; Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis; Frontiers Media; Frontiers in Plant Science; 10; 1-2020; 1-20
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fpls.2019.01635/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2019.01635
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195717219123200
spelling Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in ArabidopsisBorniego, María LucíaSteelheart Molina, Maria CharlotteGuiamet, Juan JoséMartinez, Dana EthelAPOPLASTAPOPLASTIC FLUIDEXTRACELLULAR PHPATHOGENESIS-RELATED PROTEINPRSECRETOMESENESCENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The apoplast, i.e. the cellular compartment external to the plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar to the apoplast alkalization in response to abiotic stresses. The levels of 159 proteins decrease, whereas 24 proteins increase in relative abundance in the apoplast of senescing leaves. Around half of the apoplastic proteins of non-senescent leaves contain a N-terminal signal peptide for secretion, while all the identified senescence-associated apoplastic proteins contain the signal peptide. Several of the apoplastic proteins that accumulate during senescence also accumulate in stress responses, suggesting that the apoplast may constitute a compartment where developmental and stress-related programs overlap. Other senescence-related apoplastic proteins are involved in cell wall modifications, proteolysis, carbohydrate, ROS and amino acid metabolism, signaling, lipid transport, etc. The most abundant senescence-associated apoplastic proteins, PR2 and PR5 (e.g. pathogenesis related proteins PR2 and PR5) are related to leaf aging rather than to the chloroplast degradation program, as their levels increase only in leaves undergoing developmental senescence, but not in dark-induced senescent leaves. Changes in the apoplastic space may be relevant for signaling and molecular trafficking underlying senescence.Fil: Borniego, María Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Steelheart Molina, Maria Charlotte. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Guiamet, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Martinez, Dana Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFrontiers Media2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180822Borniego, María Lucía; Steelheart Molina, Maria Charlotte; Guiamet, Juan José; Martinez, Dana Ethel; Physiological and Proteomic Changes in the Apoplast Accompany Leaf Senescence in Arabidopsis; Frontiers Media; Frontiers in Plant Science; 10; 1-2020; 1-201664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fpls.2019.01635/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2019.01635info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:01:19Zoai:ri.conicet.gov.ar:11336/180822instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:01:20.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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