Identification of a cluster of strains bearing a new adhesin among genetically diverse enterotoxigenic Escherichia coli isolates of serogroup O20

About one-third of the enterotoxigenic Escherichia coli isolates lack any of the known colonization factors. Among this group, those of serogroup O20 are the most frequently isolated in Argentina. By combining analysis of adhesion to Caco-2 cells, random amplified polymorphic DNA, and pulsed-field g...

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Detalles Bibliográficos
Autores: Pichel, Mariana, Binsztein, Norma, Gutkind, Gabriel, Viboud, Gloria I.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2001
País:Argentina
Institución:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
Repositorio:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Idioma:inglés
OAI Identifier:oai:sgc.anlis.gob.ar:123456789/288
Acceso en línea:http://sgc.anlis.gob.ar/handle/123456789/288
http://jcm.asm.org/content/39/2/782.full.pdf+html
Access Level:acceso abierto
Palabra clave:Escherichia coli
Proteínas de Escherichia coli
Infecciones por Escherichia coli
Análisis por Conglomerados
Adhesinas de Escherichia coli
Electroforesis en Gel de Campo Pulsado
Variación Genética
Serotipificación
Descripción
Sumario:About one-third of the enterotoxigenic Escherichia coli isolates lack any of the known colonization factors. Among this group, those of serogroup O20 are the most frequently isolated in Argentina. By combining analysis of adhesion to Caco-2 cells, random amplified polymorphic DNA, and pulsed-field gel electrophoresis techniques, we were able to identify three sets of closely related strains with different binding properties. Further analysis of the most prevalent group revealed that all these isolates expressed the recently described adhesin CS22.