Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats

Nonischemic 5/6 nephrectomized rat (NefR) is a model of chronic kidney disease. However, little is known about vascular dysfunction and its relation with hypertension in NefR. Aims. To evaluate possible alterations of endothelial function, NObioavailability, and basal tone in aorta from NefR and the...

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Authors: Marañón, Rodrigo Oscar, Joo Turoni, Claudio Martín, Karbiner, María Sofía, Salas, Nicolás Martín, Peral, Maria de Los Angeles
Format: article
Status:Published version
Publication Date:2013
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/22010
Online Access:http://hdl.handle.net/11336/22010
Access Level:Open access
Keyword:NITRIC OXIDE
NEPHRECTOMY
BASAL TONE
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
title Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
spellingShingle Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
Marañón, Rodrigo Oscar
NITRIC OXIDE
NEPHRECTOMY
BASAL TONE
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
title_full Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
title_fullStr Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
title_full_unstemmed Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
title_sort Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
dc.creator.none.fl_str_mv Marañón, Rodrigo Oscar
Joo Turoni, Claudio Martín
Karbiner, María Sofía
Salas, Nicolás Martín
Peral, Maria de Los Angeles
author Marañón, Rodrigo Oscar
author_facet Marañón, Rodrigo Oscar
Joo Turoni, Claudio Martín
Karbiner, María Sofía
Salas, Nicolás Martín
Peral, Maria de Los Angeles
author_role author
author2 Joo Turoni, Claudio Martín
Karbiner, María Sofía
Salas, Nicolás Martín
Peral, Maria de Los Angeles
author2_role author
author
author
author
dc.subject.none.fl_str_mv NITRIC OXIDE
NEPHRECTOMY
BASAL TONE
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic NITRIC OXIDE
NEPHRECTOMY
BASAL TONE
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Nonischemic 5/6 nephrectomized rat (NefR) is a model of chronic kidney disease. However, little is known about vascular dysfunction and its relation with hypertension in NefR. Aims. To evaluate possible alterations of endothelial function, NObioavailability, and basal tone in aorta from NefR and the role of oxidative stress. Sprague Dawley rats were divided into sham rats (SR), NefR, and NefR treated with tempol (NefR-T). Mean arterial pressure (MAP) and renal function were determined. In isolated aortic rings the following was measured: 1-endothelial function, 2-basal tone, 3-NO levels, 4-membrane potential (MP), and 5-oxidative stress. NefR increased MAP (SR: 119 ± 4 mmHg; =7; NefR: 169 ± 6; =8; < 0.001). Tempol did not modify MAP (NefR-T: 168 ± 10; =6; < 0.001). NefR showed endothelial dysfunction, increased basal tone and decreased NO levels (SR: 32 ± 2 nA; =7, NefR: 10 ± 2; =8; < 0.001). In both in vitro and in vivo tempol improves basal tone, NO levels, and MP. Oxidative stress in NefR was reverted in NefR-T. We described, for the first time, that aorta from NefR presented increased basal tone related to endothelial dysfunction and decreased NO-bioavailability. The fact that tempol improves NO-contents and basal tone, without decrease MAP, indicates that oxidative stress could be implicated early and independently to hypertension, in the vascular alterations.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22010
Marañón, Rodrigo Oscar; Joo Turoni, Claudio Martín; Karbiner, María Sofía; Salas, Nicolás Martín; Peral, Maria de Los Angeles; Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats; Hindawi Publishing Corporation; International Journal of Hypertension; 2013; 4-2013; 1-8; 863067
2090-0384
2090-0392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22010
identifier_str_mv Marañón, Rodrigo Oscar; Joo Turoni, Claudio Martín; Karbiner, María Sofía; Salas, Nicolás Martín; Peral, Maria de Los Angeles; Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats; Hindawi Publishing Corporation; International Journal of Hypertension; 2013; 4-2013; 1-8; 863067
2090-0384
2090-0392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/863067
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ijhy/2013/863067/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196053292974080
spelling Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive ratsMarañón, Rodrigo OscarJoo Turoni, Claudio MartínKarbiner, María SofíaSalas, Nicolás MartínPeral, Maria de Los AngelesNITRIC OXIDENEPHRECTOMYBASAL TONEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nonischemic 5/6 nephrectomized rat (NefR) is a model of chronic kidney disease. However, little is known about vascular dysfunction and its relation with hypertension in NefR. Aims. To evaluate possible alterations of endothelial function, NObioavailability, and basal tone in aorta from NefR and the role of oxidative stress. Sprague Dawley rats were divided into sham rats (SR), NefR, and NefR treated with tempol (NefR-T). Mean arterial pressure (MAP) and renal function were determined. In isolated aortic rings the following was measured: 1-endothelial function, 2-basal tone, 3-NO levels, 4-membrane potential (MP), and 5-oxidative stress. NefR increased MAP (SR: 119 ± 4 mmHg; =7; NefR: 169 ± 6; =8; < 0.001). Tempol did not modify MAP (NefR-T: 168 ± 10; =6; < 0.001). NefR showed endothelial dysfunction, increased basal tone and decreased NO levels (SR: 32 ± 2 nA; =7, NefR: 10 ± 2; =8; < 0.001). In both in vitro and in vivo tempol improves basal tone, NO levels, and MP. Oxidative stress in NefR was reverted in NefR-T. We described, for the first time, that aorta from NefR presented increased basal tone related to endothelial dysfunction and decreased NO-bioavailability. The fact that tempol improves NO-contents and basal tone, without decrease MAP, indicates that oxidative stress could be implicated early and independently to hypertension, in the vascular alterations.Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina. University Of Mississippi; Estados UnidosFil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Karbiner, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Salas, Nicolás Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaHindawi Publishing Corporation2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22010Marañón, Rodrigo Oscar; Joo Turoni, Claudio Martín; Karbiner, María Sofía; Salas, Nicolás Martín; Peral, Maria de Los Angeles; Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats; Hindawi Publishing Corporation; International Journal of Hypertension; 2013; 4-2013; 1-8; 8630672090-03842090-0392CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2013/863067info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ijhy/2013/863067/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:13:08Zoai:ri.conicet.gov.ar:11336/22010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:13:08.347CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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