A green approach for Ochratoxin A determination in coffee infusions
Ochratoxin A (OTA) is a secondary metabolite of filamentous fungi and is considered within the 2B group by IARC. OTA can be found in a variety of food commodities and beverages. In this study, a new methodology for the detection and quantification of OTA in coffee beverages was developed and validat...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/220574 |
| Acceso en línea: | http://hdl.handle.net/11336/220574 |
| Access Level: | acceso abierto |
| Palabra clave: | OCHRATOXIN A INFUSIONS COFFE SAMPLES DLLME-SFO UHPLC-(ESI+)-MS/MS GREEN QUALITY EVALUATION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | Ochratoxin A (OTA) is a secondary metabolite of filamentous fungi and is considered within the 2B group by IARC. OTA can be found in a variety of food commodities and beverages. In this study, a new methodology for the detection and quantification of OTA in coffee beverages was developed and validated. The extraction procedure was based on dispersive liquid-liquid microextraction with solidification of a floating organic drop (DLLME-SFO). The extract was analyzed by UHPLC-(ESI+)-MS/MS. The methodology was sensitive, with a LOD and LOQ of 0.3 and 0.9 ng/mL, respectively, and linearity was demonstrated between 0.3 and 70 ng/mL, with a correlation coefficient (R2) of 0,9962. The relative standard deviations varied from 1.1 to 8.7% (n=3). In addition, satisfactory extraction relative recovery was obtained (88.3%, with an enrichment factor of 7-folds). This methodology was evaluated through two green analytical metrics: the Green Certificate and the AGREE software. Thus, an A-category and 0.54 points were obtained, respectively. Finally, the methodology was applied to commercial sugar added-roast coffee (coffee bag) samples. |
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