B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy

Osteoarticular brucellosis is the most common localization of human active disease. Osteoblasts are specialized mesenchymal-derived cells involved in bone formation and are considered as professional mineralizing cells. Autophagy has been involved in osteoblast metabolism. The present study demonstr...

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Detalles Bibliográficos
Autores: Pesce Viglietti, Ayelén Ivana, Gentilini, Maria Virginia, Arriola Benitez, Paula Constanza, Giambartolomei, Guillermo Hernan, Delpino, María Victoria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/94153
Acceso en línea:http://hdl.handle.net/11336/94153
Access Level:acceso abierto
Palabra clave:AUTOPHAGY
BONE
BRUCELLA
OSTEOBLAST
OSTEOBLAST ACTIVITY
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_dfb60a8dc7f7b6c4ff4e9c0979be3347
oai_identifier_str oai:ri.conicet.gov.ar:11336/94153
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
title B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
spellingShingle B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
Pesce Viglietti, Ayelén Ivana
AUTOPHAGY
BONE
BRUCELLA
OSTEOBLAST
OSTEOBLAST ACTIVITY
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
title_full B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
title_fullStr B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
title_full_unstemmed B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
title_sort B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy
dc.creator.none.fl_str_mv Pesce Viglietti, Ayelén Ivana
Gentilini, Maria Virginia
Arriola Benitez, Paula Constanza
Giambartolomei, Guillermo Hernan
Delpino, María Victoria
author Pesce Viglietti, Ayelén Ivana
author_facet Pesce Viglietti, Ayelén Ivana
Gentilini, Maria Virginia
Arriola Benitez, Paula Constanza
Giambartolomei, Guillermo Hernan
Delpino, María Victoria
author_role author
author2 Gentilini, Maria Virginia
Arriola Benitez, Paula Constanza
Giambartolomei, Guillermo Hernan
Delpino, María Victoria
author2_role author
author
author
author
dc.subject.none.fl_str_mv AUTOPHAGY
BONE
BRUCELLA
OSTEOBLAST
OSTEOBLAST ACTIVITY
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic AUTOPHAGY
BONE
BRUCELLA
OSTEOBLAST
OSTEOBLAST ACTIVITY
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Osteoarticular brucellosis is the most common localization of human active disease. Osteoblasts are specialized mesenchymal-derived cells involved in bone formation and are considered as professional mineralizing cells. Autophagy has been involved in osteoblast metabolism. The present study demonstrates that Brucella abortus infection induces the activation of the autophagic pathway in osteoblast cells. Autophagy was revealed by upregulation of LC3II/LC3I ratio and Beclin-1 expression as well as inhibition of p62 expression in infected cells. Induction of autophagy was also corroborated by using the pharmacological inhibitors wortmannin, a PI 3-kinase inhibitor, and leupeptin plus E64 (inhibitors of lysosomal proteases). Autophagy induction create a microenvironment that modifies osteoblast metabolism by the inhibition of the deposition of organic and mineral matrix, the induction of matrix metalloproteinase (MMP)-2, osteopontin, and RANKL secretion leading to bone loss. Accordingly, autophagy is also involved in the down-modulation of the master transcription factor in bone formation osterix during B. abortus infection. Taking together our results indicate that B. abortus induces the activation of autophagy pathway in osteoblast cells and this activation is involved in the modulation of osteoblast function and bone formation.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94153
Pesce Viglietti, Ayelén Ivana; Gentilini, Maria Virginia; Arriola Benitez, Paula Constanza; Giambartolomei, Guillermo Hernan; Delpino, María Victoria; B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy; Frontiers Media SA; Frontiers in cellular and infection microbiology; 8; 12-2018; 1-11
2235-2988
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94153
identifier_str_mv Pesce Viglietti, Ayelén Ivana; Gentilini, Maria Virginia; Arriola Benitez, Paula Constanza; Giambartolomei, Guillermo Hernan; Delpino, María Victoria; B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy; Frontiers Media SA; Frontiers in cellular and infection microbiology; 8; 12-2018; 1-11
2235-2988
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fcimb.2018.00425/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2018.00425
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling B. Abortus Modulates Osteoblast Function Through the Induction of AutophagyPesce Viglietti, Ayelén IvanaGentilini, Maria VirginiaArriola Benitez, Paula ConstanzaGiambartolomei, Guillermo HernanDelpino, María VictoriaAUTOPHAGYBONEBRUCELLAOSTEOBLASTOSTEOBLAST ACTIVITYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Osteoarticular brucellosis is the most common localization of human active disease. Osteoblasts are specialized mesenchymal-derived cells involved in bone formation and are considered as professional mineralizing cells. Autophagy has been involved in osteoblast metabolism. The present study demonstrates that Brucella abortus infection induces the activation of the autophagic pathway in osteoblast cells. Autophagy was revealed by upregulation of LC3II/LC3I ratio and Beclin-1 expression as well as inhibition of p62 expression in infected cells. Induction of autophagy was also corroborated by using the pharmacological inhibitors wortmannin, a PI 3-kinase inhibitor, and leupeptin plus E64 (inhibitors of lysosomal proteases). Autophagy induction create a microenvironment that modifies osteoblast metabolism by the inhibition of the deposition of organic and mineral matrix, the induction of matrix metalloproteinase (MMP)-2, osteopontin, and RANKL secretion leading to bone loss. Accordingly, autophagy is also involved in the down-modulation of the master transcription factor in bone formation osterix during B. abortus infection. Taking together our results indicate that B. abortus induces the activation of autophagy pathway in osteoblast cells and this activation is involved in the modulation of osteoblast function and bone formation.Fil: Pesce Viglietti, Ayelén Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Gentilini, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Arriola Benitez, Paula Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Giambartolomei, Guillermo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Delpino, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFrontiers Media SA2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94153Pesce Viglietti, Ayelén Ivana; Gentilini, Maria Virginia; Arriola Benitez, Paula Constanza; Giambartolomei, Guillermo Hernan; Delpino, María Victoria; B. Abortus Modulates Osteoblast Function Through the Induction of Autophagy; Frontiers Media SA; Frontiers in cellular and infection microbiology; 8; 12-2018; 1-112235-2988CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fcimb.2018.00425/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2018.00425info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:33:12Zoai:ri.conicet.gov.ar:11336/94153instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:33:13.219CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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