Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism
Summary Objetive The purpose of this study was to demonstrate that Sal-T is a reliable biomarker of androgen status in the diagnosis of male hypogonadism. Design In order to validate the salivary testosterone assay (Sal-T), its reproducibility , the agreement with serum free testosterone levels (Fre...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/111684 |
| Acceso en línea: | http://hdl.handle.net/11336/111684 |
| Access Level: | acceso abierto |
| Palabra clave: | salivary testosterone diagnosis male hypogonadism https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
| Sumario: | Summary Objetive The purpose of this study was to demonstrate that Sal-T is a reliable biomarker of androgen status in the diagnosis of male hypogonadism. Design In order to validate the salivary testosterone assay (Sal-T), its reproducibility , the agreement with serum free testosterone levels (Free-T) , the correlation with other circulating androgen markers ( bioavailable testosterone , total testosterone) and cut off values were defined. Patients and Methods We studied 52 eugonadic (E) and 20 hypogonadic (Hy) men. Sal-T was assayed using an adapted radioimmunoassay for serum testosterone. Sal-T concentrations were compared in 9 cases before and after citric acid stimulation of salivary flow-rate. Free-T and bioavailable testosterone (Bio-T) were calculated by Vermeulen equation and SHBG were determined by binding assay. Results Sal-T did not depend on salivary flow-rate and morning samples from 7.00 to 9.00 h were stable. Agreement between Sal-T and Free-T measurements was confirmed in all subjects . Sal-T levels correlated positively with all circulating androgens , showing the best correlation with Free-T in E (r=0.92) as well as in Hy (r=0.97).A cutoff value for Sal-T £ 0.195 nM showed 100% of sensibility and specificity to rule out hypogonadism. Conclusions Our data showed that Sal-T is a reliable marker of testosterone bioavailability. The results support the inclusion of this biomarker as a non invasive approach in the diagnosis of male androgen deficiency. |
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