Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array

Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modificatio...

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Autores: Peinetti, Ana Sol, Ceretti, Helena Margarita, Mizrahi, Martin Daniel, González, Graciela Alicia, Ramírez, Silvana Andrea María, Requejo, Felix Gregorio, Montserrat, Javier Marcelo, Battaglini, Fernando
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/88671
Acceso en línea:http://hdl.handle.net/11336/88671
Access Level:acceso abierto
Palabra clave:AQC
DNA
EXAFS
Nanoparticles
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
id AR_dec830f25d3cc2fae76e7fd84abdbb22
oai_identifier_str oai:ri.conicet.gov.ar:11336/88671
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
title Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
spellingShingle Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
Peinetti, Ana Sol
AQC
DNA
EXAFS
Nanoparticles
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
title_short Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
title_full Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
title_fullStr Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
title_full_unstemmed Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
title_sort Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
dc.creator.none.fl_str_mv Peinetti, Ana Sol
Ceretti, Helena Margarita
Mizrahi, Martin Daniel
González, Graciela Alicia
Ramírez, Silvana Andrea María
Requejo, Felix Gregorio
Montserrat, Javier Marcelo
Battaglini, Fernando
author Peinetti, Ana Sol
author_facet Peinetti, Ana Sol
Ceretti, Helena Margarita
Mizrahi, Martin Daniel
González, Graciela Alicia
Ramírez, Silvana Andrea María
Requejo, Felix Gregorio
Montserrat, Javier Marcelo
Battaglini, Fernando
author_role author
author2 Ceretti, Helena Margarita
Mizrahi, Martin Daniel
González, Graciela Alicia
Ramírez, Silvana Andrea María
Requejo, Felix Gregorio
Montserrat, Javier Marcelo
Battaglini, Fernando
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AQC
DNA
EXAFS
Nanoparticles
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
topic AQC
DNA
EXAFS
Nanoparticles
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
description Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modification strategies on the interplay between the meso-organization and the molecular recognition properties of a 27-mer DNA strand. This DNA strand is functionalized with different sulfur-containing moieties and immobilized on 2 nm gold nanoparticles confined on a nanoporous alumina, working the whole system as an electrode array. Surface coverages were determined by EXAFS and the performance as recognition elements for impedance-based sensors is evaluated. Our results prove that low DNA coverages on the confined nanoparticles prompt to a more sensitive response, showing the relevance in avoiding the DNA strand overcrowding. The system was able to determine a concentration as low as 100 pM of the complementary strand, thus introducing the foundations for the construction of label-free genosensors at the nanometer scale.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88671
Peinetti, Ana Sol; Ceretti, Helena Margarita; Mizrahi, Martin Daniel; González, Graciela Alicia; Ramírez, Silvana Andrea María; et al.; Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array; Elsevier Science Sa; Bioelectrochemistry; 121; 6-2018; 169-175
1567-5394
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88671
identifier_str_mv Peinetti, Ana Sol; Ceretti, Helena Margarita; Mizrahi, Martin Daniel; González, Graciela Alicia; Ramírez, Silvana Andrea María; et al.; Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array; Elsevier Science Sa; Bioelectrochemistry; 121; 6-2018; 169-175
1567-5394
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1567539417305868
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2018.02.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194883836084224
spelling Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel arrayPeinetti, Ana SolCeretti, Helena MargaritaMizrahi, Martin DanielGonzález, Graciela AliciaRamírez, Silvana Andrea MaríaRequejo, Felix GregorioMontserrat, Javier MarceloBattaglini, FernandoAQCDNAEXAFSNanoparticleshttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modification strategies on the interplay between the meso-organization and the molecular recognition properties of a 27-mer DNA strand. This DNA strand is functionalized with different sulfur-containing moieties and immobilized on 2 nm gold nanoparticles confined on a nanoporous alumina, working the whole system as an electrode array. Surface coverages were determined by EXAFS and the performance as recognition elements for impedance-based sensors is evaluated. Our results prove that low DNA coverages on the confined nanoparticles prompt to a more sensitive response, showing the relevance in avoiding the DNA strand overcrowding. The system was able to determine a concentration as low as 100 pM of the complementary strand, thus introducing the foundations for the construction of label-free genosensors at the nanometer scale.Fil: Peinetti, Ana Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Ceretti, Helena Margarita. Universidad Nacional de General Sarmiento; ArgentinaFil: Mizrahi, Martin Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: González, Graciela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Ramírez, Silvana Andrea María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Requejo, Felix Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Montserrat, Javier Marcelo. Universidad Nacional de General Sarmiento; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Battaglini, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaElsevier Science Sa2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88671Peinetti, Ana Sol; Ceretti, Helena Margarita; Mizrahi, Martin Daniel; González, Graciela Alicia; Ramírez, Silvana Andrea María; et al.; Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array; Elsevier Science Sa; Bioelectrochemistry; 121; 6-2018; 169-1751567-5394CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1567539417305868info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2018.02.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:38:39Zoai:ri.conicet.gov.ar:11336/88671instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:38:39.933CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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