Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathoge...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | Argentina |
| Institución: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositorio: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglés |
| OAI Identifier: | paperaa:paper_00992240_v76_n3_p974_PradoAcosta |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta |
| Access Level: | acceso abierto |
| Palabra clave: | Bacilli Bacillus cereus Bacteriology Cytology Hydrolases Salmonella Bacterial growth Gram-positive bacterium Hydrolase activities Lactobacillus acidophilus Proton-motive forces Salmonella enterica Staphylococcus aureus Synergistic effect Antibiotics bacterium enzyme activity growth rate membrane surface layer synergism Bacteria (microorganisms) Negibacteria Posibacteria |
| Sumario: | We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved. |
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