Microscopic and PCR-based detection of microsporidia spores in human stool samples = Detección microscópica y molecular de esporas de microsporidios en muestras de heces humanas
Microsporidia are obligate intracellular fungi with a remarkable ability to infect a wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is the most frequently microsporidia reported worldwide, and mainly associated with chronic diarrhea and wasting syndrome in AIDS pati...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2020 |
| País: | Argentina |
| Institución: | Instituto Nacional de Tecnología Agropecuaria |
| Repositorio: | INTA Digital (INTA) |
| Idioma: | inglés |
| OAI Identifier: | oai:localhost:20.500.12123/7583 |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7583 https://www.sciencedirect.com/science/article/pii/S0325754120300298 https://doi.org/10.1016/j.ram.2020.04.005 |
| Access Level: | acceso abierto |
| Palabra clave: | Microsporidiosis Microspora Infecciones por Protozoos PCR Heces Género Humano Protozoal Infections Faeces Humans Microsporidios Microsporidia Enterocytozoon bieneusi |
| Sumario: | Microsporidia are obligate intracellular fungi with a remarkable ability to infect a wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is the most frequently microsporidia reported worldwide, and mainly associated with chronic diarrhea and wasting syndrome in AIDS patients. Microscopy and PCR-based detection techniques are effective for diagnosis and identification of species and genotypes; however, these methods should be standardized in each laboratory. In this study, we performed microscopy and nested PCR techniques with PCR product sequencing to detect E. bieneusi in human stool samples. These techniques, if applied together, might prove useful for diagnosis and future epidemiological studies of intestinal microsporidiosis in Argentina. |
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