Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films

A porphyrin-fullerene C60 dyad (TCP-C60) substituted by carbazoyl groups was used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers, holding fullerene units. The properties of this film were...

Descripción completa

Detalles Bibliográficos
Autores: Ballatore, María Belén, Durantini, Javier Esteban, Gsponer, Natalia Soledad, Suárez Ramanzin, Maria Belen, Gervaldo, Miguel Andres, Otero, Luis Alberto, Spesia, Mariana Belen, Milanesio, María Elisa, Durantini, Edgardo Néstor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/180210
Acceso en línea:http://hdl.handle.net/11336/180210
Access Level:acceso abierto
Palabra clave:POLYMERIC FILMS
PORPHYRIN
FULLERENE
DYAD
PHOTODYNAMIC INACTIVATION
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
id AR_dcb87beef1be4e8a229792e8ebfbf5da
oai_identifier_str oai:ri.conicet.gov.ar:11336/180210
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
title Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
spellingShingle Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
Ballatore, María Belén
POLYMERIC FILMS
PORPHYRIN
FULLERENE
DYAD
PHOTODYNAMIC INACTIVATION
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
title_full Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
title_fullStr Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
title_full_unstemmed Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
title_sort Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films
dc.creator.none.fl_str_mv Ballatore, María Belén
Durantini, Javier Esteban
Gsponer, Natalia Soledad
Suárez Ramanzin, Maria Belen
Gervaldo, Miguel Andres
Otero, Luis Alberto
Spesia, Mariana Belen
Milanesio, María Elisa
Durantini, Edgardo Néstor
author Ballatore, María Belén
author_facet Ballatore, María Belén
Durantini, Javier Esteban
Gsponer, Natalia Soledad
Suárez Ramanzin, Maria Belen
Gervaldo, Miguel Andres
Otero, Luis Alberto
Spesia, Mariana Belen
Milanesio, María Elisa
Durantini, Edgardo Néstor
author_role author
author2 Durantini, Javier Esteban
Gsponer, Natalia Soledad
Suárez Ramanzin, Maria Belen
Gervaldo, Miguel Andres
Otero, Luis Alberto
Spesia, Mariana Belen
Milanesio, María Elisa
Durantini, Edgardo Néstor
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv POLYMERIC FILMS
PORPHYRIN
FULLERENE
DYAD
PHOTODYNAMIC INACTIVATION
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic POLYMERIC FILMS
PORPHYRIN
FULLERENE
DYAD
PHOTODYNAMIC INACTIVATION
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description A porphyrin-fullerene C60 dyad (TCP-C60) substituted by carbazoyl groups was used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers, holding fullerene units. The properties of this film were compared with those formed by layers of TCP/TCP-C60 and TCP/ZnTCP. Absorption spectra of the films presented the Soret and Q bands of the corresponding porphyrins. The TCP-C60 film produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1δg) production in water. In addition, the TCP-C60 film induced the reduction of nitro blue tetrazolium to diformazan in the presence of NADH, indicating the formation of superoxide anion radical. Moreover, photooxidation of L-tryptophan mediated by TCP-C60 films was found in water. In biological media, photoinactivation of Staphylococcus aureus was evaluated depositing a drop with 2.5 × 103 cells on the films. After 30 min irradiation, no colony formation was detected using TCP-C60 or TCP/TCP-C60 films. Furthermore, photocytotoxic activity was observed in cell suspensions of S. aureus and Escherichia coli. The irradiated TCP-C60 film produced a 4 log decrease of S. aureus survival after 30 min. Also, a 4 log reduction of E. coli viability was obtained using the TCP-C60 film after 60 min irradiation. Therefore, the TCP-C60 film is an interesting and versatile photodynamic active surface to eradicate bacteria.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180210
Ballatore, María Belén; Durantini, Javier Esteban; Gsponer, Natalia Soledad; Suárez Ramanzin, Maria Belen; Gervaldo, Miguel Andres; et al.; Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films; American Chemical Society; Environmental Science & Technology; 49; 12; 5-2015; 7456-7463
0013-936X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180210
identifier_str_mv Ballatore, María Belén; Durantini, Javier Esteban; Gsponer, Natalia Soledad; Suárez Ramanzin, Maria Belen; Gervaldo, Miguel Andres; et al.; Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films; American Chemical Society; Environmental Science & Technology; 49; 12; 5-2015; 7456-7463
0013-936X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.est.5b01407
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.est.5b01407
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196440453447680
spelling Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric filmsBallatore, María BelénDurantini, Javier EstebanGsponer, Natalia SoledadSuárez Ramanzin, Maria BelenGervaldo, Miguel AndresOtero, Luis AlbertoSpesia, Mariana BelenMilanesio, María ElisaDurantini, Edgardo NéstorPOLYMERIC FILMSPORPHYRINFULLERENEDYADPHOTODYNAMIC INACTIVATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A porphyrin-fullerene C60 dyad (TCP-C60) substituted by carbazoyl groups was used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers, holding fullerene units. The properties of this film were compared with those formed by layers of TCP/TCP-C60 and TCP/ZnTCP. Absorption spectra of the films presented the Soret and Q bands of the corresponding porphyrins. The TCP-C60 film produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1δg) production in water. In addition, the TCP-C60 film induced the reduction of nitro blue tetrazolium to diformazan in the presence of NADH, indicating the formation of superoxide anion radical. Moreover, photooxidation of L-tryptophan mediated by TCP-C60 films was found in water. In biological media, photoinactivation of Staphylococcus aureus was evaluated depositing a drop with 2.5 × 103 cells on the films. After 30 min irradiation, no colony formation was detected using TCP-C60 or TCP/TCP-C60 films. Furthermore, photocytotoxic activity was observed in cell suspensions of S. aureus and Escherichia coli. The irradiated TCP-C60 film produced a 4 log decrease of S. aureus survival after 30 min. Also, a 4 log reduction of E. coli viability was obtained using the TCP-C60 film after 60 min irradiation. Therefore, the TCP-C60 film is an interesting and versatile photodynamic active surface to eradicate bacteria.Fil: Ballatore, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Durantini, Javier Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Gsponer, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Suárez Ramanzin, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Gervaldo, Miguel Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Otero, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Spesia, Mariana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Milanesio, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaAmerican Chemical Society2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180210Ballatore, María Belén; Durantini, Javier Esteban; Gsponer, Natalia Soledad; Suárez Ramanzin, Maria Belen; Gervaldo, Miguel Andres; et al.; Photodynamic inactivation of bacteria using novel electrogenerated porphyrin-fullerene C60 polymeric films; American Chemical Society; Environmental Science & Technology; 49; 12; 5-2015; 7456-74630013-936XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.est.5b01407info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.est.5b01407info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:27:55Zoai:ri.conicet.gov.ar:11336/180210instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:27:55.751CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,811543

Similares en LA Referencia