Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
Salicylic acid (SA) is a phytohormone with a central role in plant protection against various types of stresses. Exogenous application of SA in plants often requires a fine adjustment of the pH in the applied solution as well as frequent foliar sprays with a rigorous handling of doses. This work inv...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/140295 |
| Acceso en línea: | http://hdl.handle.net/11336/140295 |
| Access Level: | acceso abierto |
| Palabra clave: | BENTONITE PSEUDOMONAS SYRINGAE PV. TOMATO DC3000 SALICYLIC ACID SOLANUM LYCOPERSICUM https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | Salicylic acid (SA) is a phytohormone with a central role in plant protection against various types of stresses. Exogenous application of SA in plants often requires a fine adjustment of the pH in the applied solution as well as frequent foliar sprays with a rigorous handling of doses. This work investigates the preparation and characterization of nanocomposites composed of bentonite clays (Bent) and SA as bio-active to produce an efficient and stable hormonal formulation to be used in agriculture. Bent-SA was obtained via cationic exchange reactions of natural Bent with an aqueous solution of SA. The physicochemical properties of Bent-SA were studied in terms of Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD) and Thermogravimetric Analysis (TGA). SA was intercalated in a 3.65% w/w interacting by electrostatic forces between Bent layers. The SA protective activity was investigated in Pseudomonas syringae pv. tomato DC3000 (Pto)-infected tomato plants (Solanum lycopersicum). The expression levels of pathogenesis-related proteins (PRs) were measured by real time quantitative RT-PCR. The genes PR1 and PR5 were up-regulated 2.2- and 1.23-fold, respectively, in 24 h-treated Bent-SA plants. Furthermore, PR3 protein level increased locally and systemically by Bent-SA treatment in plant tissues. The plant disease incidence was significantly reduced in Bent-SA pretreated seedlings. Analyses of DR5:GUS transgenic reporter tomato lines showed that Bent-SA effectiveness was preserved after vehiculization. It was concluded that Bent-SA can provide an environmentally friendly strategy to control tomato plants against Pto bacterial infection. |
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