Study of the effects of spray-drying on the functionality of probiotic lactobacilli

Three probiotic lactobacilli strains were spray-dried in 20% (w/v) skim milk and submitted to a simulated gastrointestinal digestion. Fresh or spray-dried cultures were administered to mice for 5 and 10 days, and Immunoglobulin A (IgA)-producing cells were enumerated in the small intestine by immuno...

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Detalhes bibliográficos
Autores: Paez, Roxana, Lavari, Luisina, Audero, Gabriela, Cuatrin, Alejandra, Zaritzky, Noemi Elisabet, Reinheimer, Jorge Alberto, Vinderola, Celso Gabriel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/1585
Acesso em linha:http://hdl.handle.net/11336/1585
Access Level:acceso abierto
Palavra-chave:IMMUNOMODULATION
IN VIVO
LACTOBACILLI
MICROSCOPY
SPRAY-DRYING
VIABILITY
https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
Descrição
Resumo:Three probiotic lactobacilli strains were spray-dried in 20% (w/v) skim milk and submitted to a simulated gastrointestinal digestion. Fresh or spray-dried cultures were administered to mice for 5 and 10 days, and Immunoglobulin A (IgA)-producing cells were enumerated in the small intestine by immunohistochemistry. Spray-drying significantly enhanced the resistance of Lactobacillus paracasei A13 and Lactobacillus casei Nad to a simulated gastrointestinal digestion (0.96 and 1.95 log orders, respectively), compared with fresh cultures. Also, a significant higher number of IgA-producing cells were induced by spray-dried cultures compared with fresh cultures. Spray-drying is a suitable, but strain-dependent, technological process for the development of probiotic cultures in skim milk with increased functionality.