Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology

In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple...

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Detalles Bibliográficos
Autores: Reinoso, Elina Beatríz, Bettera, Susana Gertrudis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/71749
Acceso en línea:http://hdl.handle.net/11336/71749
Access Level:acceso abierto
Palabra clave:LABORATORY EXERCISES
MOLECULAR EPIDEMIOLOGY
RAPD-PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Random amplified polymorphic DNA PCR in the teaching of molecular epidemiologyReinoso, Elina BeatrízBettera, Susana GertrudisLABORATORY EXERCISESMOLECULAR EPIDEMIOLOGYRAPD-PCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak.Fil: Reinoso, Elina Beatríz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bettera, Susana Gertrudis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaJohn Wiley & Sons Inc2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/71749Reinoso, Elina Beatríz; Bettera, Susana Gertrudis; Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 44; 4; 7-2016; 391-3961470-81751539-3429CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.20957info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20957info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:39:54Zoai:ri.conicet.gov.ar:11336/71749instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:39:54.734CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
title Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
spellingShingle Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
Reinoso, Elina Beatríz
LABORATORY EXERCISES
MOLECULAR EPIDEMIOLOGY
RAPD-PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
title_full Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
title_fullStr Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
title_full_unstemmed Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
title_sort Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology
dc.creator.none.fl_str_mv Reinoso, Elina Beatríz
Bettera, Susana Gertrudis
author Reinoso, Elina Beatríz
author_facet Reinoso, Elina Beatríz
Bettera, Susana Gertrudis
author_role author
author2 Bettera, Susana Gertrudis
author2_role author
dc.subject.none.fl_str_mv LABORATORY EXERCISES
MOLECULAR EPIDEMIOLOGY
RAPD-PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic LABORATORY EXERCISES
MOLECULAR EPIDEMIOLOGY
RAPD-PCR
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71749
Reinoso, Elina Beatríz; Bettera, Susana Gertrudis; Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 44; 4; 7-2016; 391-396
1470-8175
1539-3429
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71749
identifier_str_mv Reinoso, Elina Beatríz; Bettera, Susana Gertrudis; Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 44; 4; 7-2016; 391-396
1470-8175
1539-3429
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.20957
info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20957
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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