Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine

The aim of this work was to evaluate the effects of a diet depleted of amino acids (protein-free diet, or PFD), as well as the supplementation with methionine (PFD+Met), on the antioxidant status of the female mouse liver. With this purpose, cytosolic protein spots from two-dimensional non-equilibri...

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Autores: Ronchi, Virginia Paola, Giudici, Ana Marcela, Mendieta, Julieta Renee, Caballero, Veronica Jorgelina, Chisari, Andrea Nancy, Sanllorenti, Pedro M., Conde, Ruben Danilo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/13227
Acceso en línea:http://hdl.handle.net/11336/13227
Access Level:acceso abierto
Palabra clave:Mouse Liver
Protein-Free Diet
Oxidative Stress
Methionine
Antioxidant Defences
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_d7dfee1eb9ceb25e986fd1fce3ef3fdf
oai_identifier_str oai:ri.conicet.gov.ar:11336/13227
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
title Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
spellingShingle Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
Ronchi, Virginia Paola
Mouse Liver
Protein-Free Diet
Oxidative Stress
Methionine
Antioxidant Defences
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
title_full Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
title_fullStr Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
title_full_unstemmed Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
title_sort Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
dc.creator.none.fl_str_mv Ronchi, Virginia Paola
Giudici, Ana Marcela
Mendieta, Julieta Renee
Caballero, Veronica Jorgelina
Chisari, Andrea Nancy
Sanllorenti, Pedro M.
Conde, Ruben Danilo
author Ronchi, Virginia Paola
author_facet Ronchi, Virginia Paola
Giudici, Ana Marcela
Mendieta, Julieta Renee
Caballero, Veronica Jorgelina
Chisari, Andrea Nancy
Sanllorenti, Pedro M.
Conde, Ruben Danilo
author_role author
author2 Giudici, Ana Marcela
Mendieta, Julieta Renee
Caballero, Veronica Jorgelina
Chisari, Andrea Nancy
Sanllorenti, Pedro M.
Conde, Ruben Danilo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mouse Liver
Protein-Free Diet
Oxidative Stress
Methionine
Antioxidant Defences
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Mouse Liver
Protein-Free Diet
Oxidative Stress
Methionine
Antioxidant Defences
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The aim of this work was to evaluate the effects of a diet depleted of amino acids (protein-free diet, or PFD), as well as the supplementation with methionine (PFD+Met), on the antioxidant status of the female mouse liver. With this purpose, cytosolic protein spots from two-dimensional non-equilibrium pH gel electrophoresis were identified by several procedures, such as mass spectrometry, Western blot, gel matching and enzymatic activity. PFD decreased the contents of catalase (CAT), peroxiredoxin I (Prx-I), and glutathione peroxidase (GPx) by 67%, 37% and 45%, respectively. Gene expression analyses showed that PFD caused a decrease in CAT (−20%) and GPx (−30%) mRNA levels but did not change that of Prx-I. It was also found that, when compared to a normal diet, PFD increased the liver contents of both reactive oxygen species (+50%) and oxidized protein (+88%) and decreased that of glutathione (−45%). Supplementation of PFD with Met prevented these latter effects to varying degrees, whereas CAT, Prx-I and GPx mRNA levels resulted unmodified. Present results suggest that dietary amino acid deprivation deranges the liver antioxidant defences, and this can be, in part, overcome by supplementation with Met.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13227
Ronchi, Virginia Paola; Giudici, Ana Marcela; Mendieta, Julieta Renee; Caballero, Veronica Jorgelina; Chisari, Andrea Nancy; et al.; Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine; Springer; Journal Of Physiology And Biochemistry; 66; 2; 6-2010; 93-103
1138-7548
1877-8755
url http://hdl.handle.net/11336/13227
identifier_str_mv Ronchi, Virginia Paola; Giudici, Ana Marcela; Mendieta, Julieta Renee; Caballero, Veronica Jorgelina; Chisari, Andrea Nancy; et al.; Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine; Springer; Journal Of Physiology And Biochemistry; 66; 2; 6-2010; 93-103
1138-7548
1877-8755
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s13105-010-0014-x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s13105-010-0014-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194808258920448
spelling Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionineRonchi, Virginia PaolaGiudici, Ana MarcelaMendieta, Julieta ReneeCaballero, Veronica JorgelinaChisari, Andrea NancySanllorenti, Pedro M.Conde, Ruben DaniloMouse LiverProtein-Free DietOxidative StressMethionineAntioxidant Defenceshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to evaluate the effects of a diet depleted of amino acids (protein-free diet, or PFD), as well as the supplementation with methionine (PFD+Met), on the antioxidant status of the female mouse liver. With this purpose, cytosolic protein spots from two-dimensional non-equilibrium pH gel electrophoresis were identified by several procedures, such as mass spectrometry, Western blot, gel matching and enzymatic activity. PFD decreased the contents of catalase (CAT), peroxiredoxin I (Prx-I), and glutathione peroxidase (GPx) by 67%, 37% and 45%, respectively. Gene expression analyses showed that PFD caused a decrease in CAT (−20%) and GPx (−30%) mRNA levels but did not change that of Prx-I. It was also found that, when compared to a normal diet, PFD increased the liver contents of both reactive oxygen species (+50%) and oxidized protein (+88%) and decreased that of glutathione (−45%). Supplementation of PFD with Met prevented these latter effects to varying degrees, whereas CAT, Prx-I and GPx mRNA levels resulted unmodified. Present results suggest that dietary amino acid deprivation deranges the liver antioxidant defences, and this can be, in part, overcome by supplementation with Met.Fil: Ronchi, Virginia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Giudici, Ana Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Caballero, Veronica Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Chisari, Andrea Nancy. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Sanllorenti, Pedro M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaSpringer2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13227Ronchi, Virginia Paola; Giudici, Ana Marcela; Mendieta, Julieta Renee; Caballero, Veronica Jorgelina; Chisari, Andrea Nancy; et al.; Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine; Springer; Journal Of Physiology And Biochemistry; 66; 2; 6-2010; 93-1031138-75481877-8755enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s13105-010-0014-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13105-010-0014-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:36:53Zoai:ri.conicet.gov.ar:11336/13227instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:36:53.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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