Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis

Experimental autoimmune orchitis (EAO) is an organ-specific model of autoimmunity characterized by an interstitial lymphomononuclear cell infiltrate as well as sloughing and apoptosis of germ cells. EAO was induced in adult male Sprague-Dawley rats by active immunization with testicular homogenate a...

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Detalhes bibliográficos
Autores: Rival, Claudia, Theas, Maria Susana, Guazzone, Vanesa Anabella, Lustig, Livia
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2006
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/112795
Acesso em linha:http://hdl.handle.net/11336/112795
Access Level:Acceso aberto
Palavra-chave:AUTOIMMUNE ORCHITIS
GERM CELL APOPTOSIS
IL-6
IL-6R
TESTICULAR MACROPHAGES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_d5823afafcd74ebbcff8c5673bdca5fb
oai_identifier_str oai:ri.conicet.gov.ar:11336/112795
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
title Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
spellingShingle Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
Rival, Claudia
AUTOIMMUNE ORCHITIS
GERM CELL APOPTOSIS
IL-6
IL-6R
TESTICULAR MACROPHAGES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
title_full Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
title_fullStr Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
title_full_unstemmed Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
title_sort Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
dc.creator.none.fl_str_mv Rival, Claudia
Theas, Maria Susana
Guazzone, Vanesa Anabella
Lustig, Livia
author Rival, Claudia
author_facet Rival, Claudia
Theas, Maria Susana
Guazzone, Vanesa Anabella
Lustig, Livia
author_role author
author2 Theas, Maria Susana
Guazzone, Vanesa Anabella
Lustig, Livia
author2_role author
author
author
dc.subject.none.fl_str_mv AUTOIMMUNE ORCHITIS
GERM CELL APOPTOSIS
IL-6
IL-6R
TESTICULAR MACROPHAGES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic AUTOIMMUNE ORCHITIS
GERM CELL APOPTOSIS
IL-6
IL-6R
TESTICULAR MACROPHAGES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Experimental autoimmune orchitis (EAO) is an organ-specific model of autoimmunity characterized by an interstitial lymphomononuclear cell infiltrate as well as sloughing and apoptosis of germ cells. EAO was induced in adult male Sprague-Dawley rats by active immunization with testicular homogenate and adjuvants. Rats injected with saline solution and adjuvants were used as control group. The aim of this work was to study the expression of interleukin-6 (IL-6) and its receptor (IL-6R) in the testis of rats with EAO and analyze whether IL-6 could be involved in germ cell apoptosis. By immunohistochemistry, we detected IL-6 expression in testicular macrophages and Leydig cells of control and EAO rats. Sertoli cells showed IL-6 immunoreactivity in most of the seminiferous tubules of control rats, while a few IL-6+ Sertoli cells were found in the testis of rats with EAO. IL-6R immunoreactivity was observed in macrophages, Leydig and germ cells. A significant increase was noted in the number of IL-6R+ germ cells in rats with EAO compared to control rats. The content of IL-6 (ELISA) in the conditioned media obtained from testicular macrophages of rats with orchitis was significantly higher than in the control group. By immunofluorescence performed on isolated testicular macrophages, IL-6 was shown to be expressed by monocytes recently arrived from circulation (ED1+ cells), while resident macrophages (ED2+ cells) were negative. In vitro experiments (trypan blue and MTS assays) showed that IL-6 (50 ng/ml) reduced germ cell viability. We demonstrated also using the TUNEL technique that IL-6 added to cultures of seminiferous tubule segments induced apoptosis of germ cells. Our results suggest that IL-6 and IL-6R may be involved in the pathogenesis of autoimmune orchitis by promoting testicular inflammation and germ cell apoptosis.
publishDate 2006
dc.date.none.fl_str_mv 2006-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112795
Rival, Claudia; Theas, Maria Susana; Guazzone, Vanesa Anabella; Lustig, Livia; Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis; Elsevier Ireland; Journal of Reproductive Immunology; 70; 1-2; 6-2006; 43-58
0165-0378
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112795
identifier_str_mv Rival, Claudia; Theas, Maria Susana; Guazzone, Vanesa Anabella; Lustig, Livia; Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis; Elsevier Ireland; Journal of Reproductive Immunology; 70; 1-2; 6-2006; 43-58
0165-0378
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165037805001506
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jri.2005.10.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195841131446272
spelling Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitisRival, ClaudiaTheas, Maria SusanaGuazzone, Vanesa AnabellaLustig, LiviaAUTOIMMUNE ORCHITISGERM CELL APOPTOSISIL-6IL-6RTESTICULAR MACROPHAGEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Experimental autoimmune orchitis (EAO) is an organ-specific model of autoimmunity characterized by an interstitial lymphomononuclear cell infiltrate as well as sloughing and apoptosis of germ cells. EAO was induced in adult male Sprague-Dawley rats by active immunization with testicular homogenate and adjuvants. Rats injected with saline solution and adjuvants were used as control group. The aim of this work was to study the expression of interleukin-6 (IL-6) and its receptor (IL-6R) in the testis of rats with EAO and analyze whether IL-6 could be involved in germ cell apoptosis. By immunohistochemistry, we detected IL-6 expression in testicular macrophages and Leydig cells of control and EAO rats. Sertoli cells showed IL-6 immunoreactivity in most of the seminiferous tubules of control rats, while a few IL-6+ Sertoli cells were found in the testis of rats with EAO. IL-6R immunoreactivity was observed in macrophages, Leydig and germ cells. A significant increase was noted in the number of IL-6R+ germ cells in rats with EAO compared to control rats. The content of IL-6 (ELISA) in the conditioned media obtained from testicular macrophages of rats with orchitis was significantly higher than in the control group. By immunofluorescence performed on isolated testicular macrophages, IL-6 was shown to be expressed by monocytes recently arrived from circulation (ED1+ cells), while resident macrophages (ED2+ cells) were negative. In vitro experiments (trypan blue and MTS assays) showed that IL-6 (50 ng/ml) reduced germ cell viability. We demonstrated also using the TUNEL technique that IL-6 added to cultures of seminiferous tubule segments induced apoptosis of germ cells. Our results suggest that IL-6 and IL-6R may be involved in the pathogenesis of autoimmune orchitis by promoting testicular inflammation and germ cell apoptosis.Fil: Rival, Claudia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; ArgentinaFil: Theas, Maria Susana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Guazzone, Vanesa Anabella. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Lustig, Livia. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier Ireland2006-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112795Rival, Claudia; Theas, Maria Susana; Guazzone, Vanesa Anabella; Lustig, Livia; Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis; Elsevier Ireland; Journal of Reproductive Immunology; 70; 1-2; 6-2006; 43-580165-0378CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165037805001506info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jri.2005.10.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:05:39Zoai:ri.conicet.gov.ar:11336/112795instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:05:39.856CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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