Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species foun...
| Autores: | , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2005 |
| País: | Argentina |
| Recursos: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositório: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglês |
| OAI Identifier: | paperaa:paper_00052736_v1713_n1_p57_Ohtake |
| Acesso em linha: | http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake |
| Access Level: | Acceso aberto |
| Palavra-chave: | Cholesterol DPPC DPPE Freeze-dried Phase transition cholesterol dipalmitoylphosphatidylcholine dipalmitoylphosphatidylethanolamine liposome phospholipid trehalose article enthalpy freeze drying lipid composition lipid membrane phase separation phase transition priority journal temperature sensitivity thermostability 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Freeze Drying Phosphatidylethanolamines Trehalose Mammalia |
| Resumo: | A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved. |
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