Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus
An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2′-deoxyriboside and 2,6-diaminopurine riboside, respectively. Th...
| Autores: | , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2012 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/189045 |
| Acesso em linha: | http://hdl.handle.net/11336/189045 |
| Access Level: | Acceso aberto |
| Palavra-chave: | NUCLEOSIDE PHOSPHORYLASE PRODRUG PRECURSORS THERMOPHILIC MICROORGANISMS TRANSGLYCOSYLATION WHOLE CELL IMMOBILIZATION https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
| Resumo: | An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2′-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2′-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis. |
|---|