A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge

Anaplasma marginale is the most prevalent tick-borne livestock pathogen with worldwide distribution. Bovine anaplasmosis is a significant threat to cattle industry. Anaplasmosis outbreaks in endemic areas are prevented via vaccination with live A. centrale produced in splenectomized calves. Since A....

ver descrição completa

Detalhes bibliográficos
Autores: Sarli, Macarena, Novoa, María Belén, Mazzucco, Matilde N., Signorini Porchietto, Marcelo Lisandro, Echaide, Ignacio Eduardo, Torioni de Echaide, Susana Marta, Primo, Maria Evangelina
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/152114
Acesso em linha:http://hdl.handle.net/11336/152114
Access Level:acceso abierto
Palavra-chave:ANAPLASMA MARGINALE
RECOMBINANT VACCINE
TYPE IV SECRETION SYSTEN
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
id AR_d359ec2aa7a85589392954db954c4e0f
oai_identifier_str oai:ri.conicet.gov.ar:11336/152114
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
title A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
spellingShingle A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
Sarli, Macarena
ANAPLASMA MARGINALE
RECOMBINANT VACCINE
TYPE IV SECRETION SYSTEN
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
title_short A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
title_full A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
title_fullStr A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
title_full_unstemmed A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
title_sort A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
dc.creator.none.fl_str_mv Sarli, Macarena
Novoa, María Belén
Mazzucco, Matilde N.
Signorini Porchietto, Marcelo Lisandro
Echaide, Ignacio Eduardo
Torioni de Echaide, Susana Marta
Primo, Maria Evangelina
author Sarli, Macarena
author_facet Sarli, Macarena
Novoa, María Belén
Mazzucco, Matilde N.
Signorini Porchietto, Marcelo Lisandro
Echaide, Ignacio Eduardo
Torioni de Echaide, Susana Marta
Primo, Maria Evangelina
author_role author
author2 Novoa, María Belén
Mazzucco, Matilde N.
Signorini Porchietto, Marcelo Lisandro
Echaide, Ignacio Eduardo
Torioni de Echaide, Susana Marta
Primo, Maria Evangelina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANAPLASMA MARGINALE
RECOMBINANT VACCINE
TYPE IV SECRETION SYSTEN
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
topic ANAPLASMA MARGINALE
RECOMBINANT VACCINE
TYPE IV SECRETION SYSTEN
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
description Anaplasma marginale is the most prevalent tick-borne livestock pathogen with worldwide distribution. Bovine anaplasmosis is a significant threat to cattle industry. Anaplasmosis outbreaks in endemic areas are prevented via vaccination with live A. centrale produced in splenectomized calves. Since A. centrale live vaccine can carry other pathogens and cause disease in adult cattle, research efforts are directed to develop safe recombinant subunit vaccines. Previous work found that the subdominant proteins of A. marginale type IV secretion system (T4SS) and the subdominant elongation factor-Tu (Ef-Tu) were involved in the protective immunity against the experimental challenge in cattle immunized with the A. marginale outer membrane (OM). This study evaluated the immunogenicity and protection conferred by recombinant VirB9.1, VirB9.2, VirB10, VirB11, and Ef-Tu proteins cloned and expressed in E. coli. Twenty steers were randomly clustered into four groups (G) of five animals each. Cattle from G1 and G2 were immunized with a mixture of 50 μg of each recombinant protein with Quil A® or Montanide™ adjuvants, respectively. Cattle from G3 and G4 (controls) were immunized with Quil A and Montanide adjuvants, respectively. Cattle received four immunizations at three-week intervals and were challenged with 107 A. marginale-parasitized erythrocytes 42 days after the fourth immunization. After challenge, all cattle showed clinical signs, with a significant drop of packed cell volume and a significant increase of parasitized erythrocytes (p<0.05), requiring treatment with oxytetracycline to prevent death. The levels of IgG2 induced in the immunized groups did not correlate with the observed lack of protection. Additional strategies are required to evaluate the role of these proteins and their potential utility in the development of effective vaccines.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/152114
Sarli, Macarena; Novoa, María Belén; Mazzucco, Matilde N.; Signorini Porchietto, Marcelo Lisandro; Echaide, Ignacio Eduardo; et al.; A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge; Public Library of Science; Plos One; 15; 2; 2-2020; 1-14
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/152114
identifier_str_mv Sarli, Macarena; Novoa, María Belén; Mazzucco, Matilde N.; Signorini Porchietto, Marcelo Lisandro; Echaide, Ignacio Eduardo; et al.; A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge; Public Library of Science; Plos One; 15; 2; 2-2020; 1-14
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0229301
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0229301
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195873439121408
spelling A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challengeSarli, MacarenaNovoa, María BelénMazzucco, Matilde N.Signorini Porchietto, Marcelo LisandroEchaide, Ignacio EduardoTorioni de Echaide, Susana MartaPrimo, Maria EvangelinaANAPLASMA MARGINALERECOMBINANT VACCINETYPE IV SECRETION SYSTENhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Anaplasma marginale is the most prevalent tick-borne livestock pathogen with worldwide distribution. Bovine anaplasmosis is a significant threat to cattle industry. Anaplasmosis outbreaks in endemic areas are prevented via vaccination with live A. centrale produced in splenectomized calves. Since A. centrale live vaccine can carry other pathogens and cause disease in adult cattle, research efforts are directed to develop safe recombinant subunit vaccines. Previous work found that the subdominant proteins of A. marginale type IV secretion system (T4SS) and the subdominant elongation factor-Tu (Ef-Tu) were involved in the protective immunity against the experimental challenge in cattle immunized with the A. marginale outer membrane (OM). This study evaluated the immunogenicity and protection conferred by recombinant VirB9.1, VirB9.2, VirB10, VirB11, and Ef-Tu proteins cloned and expressed in E. coli. Twenty steers were randomly clustered into four groups (G) of five animals each. Cattle from G1 and G2 were immunized with a mixture of 50 μg of each recombinant protein with Quil A® or Montanide™ adjuvants, respectively. Cattle from G3 and G4 (controls) were immunized with Quil A and Montanide adjuvants, respectively. Cattle received four immunizations at three-week intervals and were challenged with 107 A. marginale-parasitized erythrocytes 42 days after the fourth immunization. After challenge, all cattle showed clinical signs, with a significant drop of packed cell volume and a significant increase of parasitized erythrocytes (p<0.05), requiring treatment with oxytetracycline to prevent death. The levels of IgG2 induced in the immunized groups did not correlate with the observed lack of protection. Additional strategies are required to evaluate the role of these proteins and their potential utility in the development of effective vaccines.Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Novoa, María Belén. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Mazzucco, Matilde N.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Torioni de Echaide, Susana Marta. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Primo, Maria Evangelina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaPublic Library of Science2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152114Sarli, Macarena; Novoa, María Belén; Mazzucco, Matilde N.; Signorini Porchietto, Marcelo Lisandro; Echaide, Ignacio Eduardo; et al.; A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge; Public Library of Science; Plos One; 15; 2; 2-2020; 1-141932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0229301info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0229301info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:06:45Zoai:ri.conicet.gov.ar:11336/152114instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:06:46.095CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,812455

Registos relacionados