Campylobacter fetus releases S-layered and immunoreactive outer membrane vesicles = Campylobacter fetus secreta vesículas de membrana externa inmunorreactivas que conservan la capa S
The study of outer membrane vesicles (OMVs) became relevant because of their probable important role in the transfer of virulence factors to host cells. Campylobacter fetus is mainly a mammal pathogen whose virulence characterization is still limited. The aim of this study was to evaluate and to cha...
| Autores: | , , , , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2021 |
| País: | Argentina |
| Recursos: | Instituto Nacional de Tecnología Agropecuaria |
| Repositório: | INTA Digital (INTA) |
| Idioma: | inglês |
| OAI Identifier: | oai:localhost:20.500.12123/10216 |
| Acesso em linha: | http://hdl.handle.net/20.500.12123/10216 https://www.sciencedirect.com/science/article/pii/S0325754121000766 https://doi.org/10.1016/j.ram.2021.06.001 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Campylobacter fetus Patogénesis Virulencia Pathogenesis Virulence Outer Membrane Vesicles Vesículas de Membrana Externa |
| Resumo: | The study of outer membrane vesicles (OMVs) became relevant because of their probable important role in the transfer of virulence factors to host cells. Campylobacter fetus is mainly a mammal pathogen whose virulence characterization is still limited. The aim of this study was to evaluate and to characterize the secretion of OMVs in this bacterium. By transmission electron microscopy, we confirmed the production of OMVs in all the strains assayed. Purified OMVs showed a spherical shape and variable size, although comparable to those of other gram-negative bacteria. We also confirmed the presence of the S-layer on the surface of the OMVs of all the strains assayed with the exception of those derived from the NTCC reference strain. In addition, we demonstrated their immunoreactivity by the dot-blot assay. Hence, C. fetus OMVs could contribute to the modulation of the host response and constitute a candidate to be evaluated as an adjuvant of current vaccines used in the veterinary field. This work represents a platform to drive future studies towards the role of these subcellular structures in C. fetus-host interaction. |
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