Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii

Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencin...

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Detalles Bibliográficos
Autores: Labrador Herrera, Gema, Pérez Pulido, Antonio J., Álvarez Marín, Rocío, Casimiro Soriguer, Carlos S., Cebrero Cangueiro, Tania, Moran Barrio, Jorgelina, Pachón, Jerónimo, Viale, Alejandro Miguel, Pachón Ibáñez, María Eugenia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/184733
Acceso en línea:http://hdl.handle.net/11336/184733
Access Level:acceso abierto
Palabra clave:ACINETOBACTER BAUMANNII
BLOODSTREAM INFECTIONS
CARBAPENEM-RESISTANCE
CARO
VIRULENCE FACTOR
WHOLE-GENOME SEQUENCING
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.

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