Fluoxetine modulates norepinephrine contractile effect on rat vas deferens

The aim of this study was to evaluate whether the antidepressant drug fluoxetine could modify rat vas deferens response to norepinephrine (NE), and to compare its effect with that of desipramine and cocaine. Results showed that 10-5 M fluoxetine produced a super-sensibility of vas deferens to NE. Th...

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Detalles Bibliográficos
Autores: Busch, Lucila, Wald, Miriam Ruth, Sterin, Leonor Josefina, Borda, Enri Santiago
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/39146
Acceso en línea:http://hdl.handle.net/11336/39146
Access Level:acceso abierto
Palabra clave:Fluoxetine
Neuronal Uptake
Norepinephrine
Vas Deferens
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
Descripción
Sumario:The aim of this study was to evaluate whether the antidepressant drug fluoxetine could modify rat vas deferens response to norepinephrine (NE), and to compare its effect with that of desipramine and cocaine. Results showed that 10-5 M fluoxetine produced a super-sensibility of vas deferens to NE. This result was the same as those obtained for 10-6 M desipramine or cocaine. Since the effect was Na+- and Cl--dependent, an inhibitory mechanism of neuronal NE transport was suggested. Fluoxetine did not modify [3H]prazosin k(d) or B(max) in rat vas deferens, reinforcing the hypothesis of a pre-synaptic site of action. On the other hand fluoxetine inhibited NE maximal effect. This inhibitory effect could be related to an antagonism of calcium entry through the voltage-dependent calcium channel, since it was partially reverted by increasing calcium concentration and, besides, the drug was able to inhibit the calcium concentration-response curve also. Contractions induced by 5-hydroxytryptamine (5-HT) were not modified in the presence of fluoxetine. It is concluded that fluoxetine modulates rat vas deferens response to low NE concentrations in the same manner as the selective inhibitor of NE neuronal uptake desipramine. This peripheral effect could participate in the modulation of the male reproductive tract observed by these drugs when used in clinical trials.