Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals = Detección molecular de coinfección con los virus de la diarrea viral bovina (BVDV) en búfalos de agua (Bubalus bubalis) serológicamente negativos

Infection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) has been confirmed in several studies by serological and molecular techniques. In order to determine the presence of persistently infected animals and circulating species and subtypes of BVDV we conducted this s...

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Bibliographic Details
Authors: Craig, María Isabel, Konig, Guido Alberto, Benitez, Daniel Francisco, Draghi, Maria Graciela
Format: article
Status:Published version
Publication Date:2015
Country:Argentina
Institution:Instituto Nacional de Tecnología Agropecuaria
Repository:INTA Digital (INTA)
Language:English
OAI Identifier:oai:localhost:20.500.12123/4446
Online Access:http://hdl.handle.net/20.500.12123/4446
https://www.sciencedirect.com/science/article/pii/S0325754115000437?via%3Dihub
Access Level:Open access
Keyword:Water Buffaloes
Infection
Bovine Diarrhoea Pestivirus
Immunological Techniques
Búfalo de Agua
Infección
Pestivirus de la Diarrea Bovina
Técnicas Inmunológicas
Bubalus Bubalis
Coinfección
BVDV
Coinfection
Description
Summary:Infection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) has been confirmed in several studies by serological and molecular techniques. In order to determine the presence of persistently infected animals and circulating species and subtypes of BVDV we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (Bossp.). Our serological results showed a high level of positivity for BVDV-1 and BVDV-2 within the buffalo herd. The molecular analyses of blood samples in serologically negative animals revealed the presence of viral nucleic acid, confirming the existence of persistent infection in the buffaloes. Cloning and sequencing of the 5′ UTR of some of these samples revealed the presence of naturally mix-infected buffaloes with at least two different subtypes (1a and 1b), and also with both BVDV species (BVDV-1 and BVDV-2).

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