Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland

Biochemical signalling events coupled to the bradykinin B2-receptor subtype, related to nitric oxide and prostaglandin E2 generation were studied in rat submandibular gland. Bradykinin stimulation of the B2-receptor triggered activation of phosphoinositide turnover, translocation of protein kinase C...

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Detalhes bibliográficos
Autores: Genaro, Ana Maria, Stranieri, Graciela M., Borda, Enri Santiago
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/39151
Acesso em linha:http://hdl.handle.net/11336/39151
Access Level:acceso abierto
Palavra-chave:Bradykinin
Cgmp
Nitric Oxide Synthase
Phosphoinositide Turnover
Prostaglandin E2
Protein Kinase C
Salivary Gland
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
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spelling Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary glandGenaro, Ana MariaStranieri, Graciela M.Borda, Enri SantiagoBradykininCgmpNitric Oxide SynthasePhosphoinositide TurnoverProstaglandin E2Protein Kinase CSalivary Glandhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Biochemical signalling events coupled to the bradykinin B2-receptor subtype, related to nitric oxide and prostaglandin E2 generation were studied in rat submandibular gland. Bradykinin stimulation of the B2-receptor triggered activation of phosphoinositide turnover, translocation of protein kinase C, stimulation of nitric oxide synthase activity, increased production of cGMP and release of prostaglandin E2. Bradykinin stimulation of nitric oxide synthase and cGMP production was blunted by agents able to interfere with calcium/calmodulin and phospholipase C activities, while a protein kinase C inhibitor was able to stimulate bradykinin action. Moreover, a specific B2-bradykinin antagonist of the reversible nitric oxide synthase inhibitor abrogated the bradykinin stimulation of nitric oxide synthase activity, cGMP accumulation and prostaglandin E2 generation. Furthermore, a specific inhibitor of phospholipase A2 blocked the bradykinin-induced prostaglandin E2 release. These results suggest that apart, from the direct effect of bradykinin as an inducer of vasopermeability, it also appears to be a vasoactive chemical mediator that triggers, through release of prostaglandin E2, a feedback mechanism that induces a protective adaptation of the gland, modulating the course of inflammation. (C) 2000 Elsevier Science Ltd.Fil: Genaro, Ana Maria. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stranieri, Graciela M.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaPergamon-Elsevier Science Ltd2000-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39151Genaro, Ana Maria; Stranieri, Graciela M.; Borda, Enri Santiago; Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland; Pergamon-Elsevier Science Ltd; Archives of Oral Biology; 45; 9; 9-2000; 723-7290003-9969CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0003-9969(00)00048-0info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003996900000480info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:35:52Zoai:ri.conicet.gov.ar:11336/39151instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:35:52.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
title Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
spellingShingle Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
Genaro, Ana Maria
Bradykinin
Cgmp
Nitric Oxide Synthase
Phosphoinositide Turnover
Prostaglandin E2
Protein Kinase C
Salivary Gland
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
title_short Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
title_full Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
title_fullStr Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
title_full_unstemmed Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
title_sort Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland
dc.creator.none.fl_str_mv Genaro, Ana Maria
Stranieri, Graciela M.
Borda, Enri Santiago
author Genaro, Ana Maria
author_facet Genaro, Ana Maria
Stranieri, Graciela M.
Borda, Enri Santiago
author_role author
author2 Stranieri, Graciela M.
Borda, Enri Santiago
author2_role author
author
dc.subject.none.fl_str_mv Bradykinin
Cgmp
Nitric Oxide Synthase
Phosphoinositide Turnover
Prostaglandin E2
Protein Kinase C
Salivary Gland
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
topic Bradykinin
Cgmp
Nitric Oxide Synthase
Phosphoinositide Turnover
Prostaglandin E2
Protein Kinase C
Salivary Gland
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
description Biochemical signalling events coupled to the bradykinin B2-receptor subtype, related to nitric oxide and prostaglandin E2 generation were studied in rat submandibular gland. Bradykinin stimulation of the B2-receptor triggered activation of phosphoinositide turnover, translocation of protein kinase C, stimulation of nitric oxide synthase activity, increased production of cGMP and release of prostaglandin E2. Bradykinin stimulation of nitric oxide synthase and cGMP production was blunted by agents able to interfere with calcium/calmodulin and phospholipase C activities, while a protein kinase C inhibitor was able to stimulate bradykinin action. Moreover, a specific B2-bradykinin antagonist of the reversible nitric oxide synthase inhibitor abrogated the bradykinin stimulation of nitric oxide synthase activity, cGMP accumulation and prostaglandin E2 generation. Furthermore, a specific inhibitor of phospholipase A2 blocked the bradykinin-induced prostaglandin E2 release. These results suggest that apart, from the direct effect of bradykinin as an inducer of vasopermeability, it also appears to be a vasoactive chemical mediator that triggers, through release of prostaglandin E2, a feedback mechanism that induces a protective adaptation of the gland, modulating the course of inflammation. (C) 2000 Elsevier Science Ltd.
publishDate 2000
dc.date.none.fl_str_mv 2000-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39151
Genaro, Ana Maria; Stranieri, Graciela M.; Borda, Enri Santiago; Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland; Pergamon-Elsevier Science Ltd; Archives of Oral Biology; 45; 9; 9-2000; 723-729
0003-9969
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39151
identifier_str_mv Genaro, Ana Maria; Stranieri, Graciela M.; Borda, Enri Santiago; Involvement of the endogenous nitric oxide signalling system in bradykinin receptor activation in rat submandibular salivary gland; Pergamon-Elsevier Science Ltd; Archives of Oral Biology; 45; 9; 9-2000; 723-729
0003-9969
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0003-9969(00)00048-0
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003996900000480
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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