AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission

1. We previously reported that angiotensin III modulates noradrenergic neurotransmission in the hypothalamus of the rat. In the present work we studied the effects of angiotensin III on norepinephrine release and tyrosine hydroxylase activity. We also investigated the receptors and intracellular pat...

Descripción completa

Detalles Bibliográficos
Autores: Rodriguez Campos, Muriel, Kadarian, Carina, Rodano, Valeria, Bianciotti, Liliana Graciela, Fernandez, Belisario Enrique, Vatta, Marcelo Sergio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/39271
Acceso en línea:http://hdl.handle.net/11336/39271
Access Level:acceso abierto
Palabra clave:Angiotensin Antagonists
Angiotensin Iii
Angiotensin Receptors
Norepinephrine Release
Renin-Angiotensin System
Tyrosine Hydroxylase Activity
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_cd93ecda618800e6ff80121ca9ef68ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/39271
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
title AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
spellingShingle AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
Rodriguez Campos, Muriel
Angiotensin Antagonists
Angiotensin Iii
Angiotensin Receptors
Norepinephrine Release
Renin-Angiotensin System
Tyrosine Hydroxylase Activity
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
title_full AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
title_fullStr AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
title_full_unstemmed AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
title_sort AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission
dc.creator.none.fl_str_mv Rodriguez Campos, Muriel
Kadarian, Carina
Rodano, Valeria
Bianciotti, Liliana Graciela
Fernandez, Belisario Enrique
Vatta, Marcelo Sergio
author Rodriguez Campos, Muriel
author_facet Rodriguez Campos, Muriel
Kadarian, Carina
Rodano, Valeria
Bianciotti, Liliana Graciela
Fernandez, Belisario Enrique
Vatta, Marcelo Sergio
author_role author
author2 Kadarian, Carina
Rodano, Valeria
Bianciotti, Liliana Graciela
Fernandez, Belisario Enrique
Vatta, Marcelo Sergio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Angiotensin Antagonists
Angiotensin Iii
Angiotensin Receptors
Norepinephrine Release
Renin-Angiotensin System
Tyrosine Hydroxylase Activity
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Angiotensin Antagonists
Angiotensin Iii
Angiotensin Receptors
Norepinephrine Release
Renin-Angiotensin System
Tyrosine Hydroxylase Activity
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description 1. We previously reported that angiotensin III modulates noradrenergic neurotransmission in the hypothalamus of the rat. In the present work we studied the effects of angiotensin III on norepinephrine release and tyrosine hydroxylase activity. We also investigated the receptors and intracellular pathways involved in angiotensin III modulation of noradrenergic transmission. 2. In rat hypothalamic tissue labeled with [3H]norepinephrine 1, 10, and 100 nM and 1 μM losartan (AT1 receptor antagonist) had no effect on basal neuronal norepinephrine release, whereas 10 and 100 nM and 1μM losartan partially diminished norepinephrine secretion evoked by 25 mM KCl. The AT2 receptor antagonist PD 123319 showed no effect either on basal or evoked norepinephrine release. The increase in both basal and evoked norepinephrine output induced by 1 μM angiotensin III was blocked by 1 μM losartan, but not by 1 μM PD 123319. 3. The phospholipase C inhibitor 5 μM neomicin inhibited the increase in basal and evoked norepinephrine release produced by 1 μM angiotensin III. 4. Tyrosine hydroxylase activity was increased by 1 μM angiotensin III and this effect was blocked by 1 μM LST and 5 μM neomicin, but not by PD 123319. On the other hand, 1 μM angiotensin III enhanced phosphatidyl inositol hydrolysis that was blocked by 1 μM losartan and 5 μM neomicin. PD 123319 (1 μM) did not affect ANG III-induced phosphatidyl inositol hydrolysis enhancement. 5. Our results confirm that angiotensin III acts as a modulator of noradrenergic transmission at the hypothalamic level through the AT1-phospholipase C pathway. This enhancement of hypothalamic noradrenergic activity suggests that angiotensin III may act as a central modulator of several biological processes regulated at this level by catecholamines, such as cardiovascular, endocrine, and autonomic functions as well as water and saline homeostasis.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39271
Rodriguez Campos, Muriel; Kadarian, Carina; Rodano, Valeria; Bianciotti, Liliana Graciela; Fernandez, Belisario Enrique; et al.; AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission; Springer/Plenum Publishers; Cellular And Molecular Neurobiology.; 20; 6; 12-2000; 747-762
0272-4340
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39271
identifier_str_mv Rodriguez Campos, Muriel; Kadarian, Carina; Rodano, Valeria; Bianciotti, Liliana Graciela; Fernandez, Belisario Enrique; et al.; AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission; Springer/Plenum Publishers; Cellular And Molecular Neurobiology.; 20; 6; 12-2000; 747-762
0272-4340
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1023/A:1007059010571
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1023%2FA%3A1007059010571
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer/Plenum Publishers
publisher.none.fl_str_mv Springer/Plenum Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194868829913088
spelling AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmissionRodriguez Campos, MurielKadarian, CarinaRodano, ValeriaBianciotti, Liliana GracielaFernandez, Belisario EnriqueVatta, Marcelo SergioAngiotensin AntagonistsAngiotensin IiiAngiotensin ReceptorsNorepinephrine ReleaseRenin-Angiotensin SystemTyrosine Hydroxylase Activityhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/31. We previously reported that angiotensin III modulates noradrenergic neurotransmission in the hypothalamus of the rat. In the present work we studied the effects of angiotensin III on norepinephrine release and tyrosine hydroxylase activity. We also investigated the receptors and intracellular pathways involved in angiotensin III modulation of noradrenergic transmission. 2. In rat hypothalamic tissue labeled with [3H]norepinephrine 1, 10, and 100 nM and 1 μM losartan (AT1 receptor antagonist) had no effect on basal neuronal norepinephrine release, whereas 10 and 100 nM and 1μM losartan partially diminished norepinephrine secretion evoked by 25 mM KCl. The AT2 receptor antagonist PD 123319 showed no effect either on basal or evoked norepinephrine release. The increase in both basal and evoked norepinephrine output induced by 1 μM angiotensin III was blocked by 1 μM losartan, but not by 1 μM PD 123319. 3. The phospholipase C inhibitor 5 μM neomicin inhibited the increase in basal and evoked norepinephrine release produced by 1 μM angiotensin III. 4. Tyrosine hydroxylase activity was increased by 1 μM angiotensin III and this effect was blocked by 1 μM LST and 5 μM neomicin, but not by PD 123319. On the other hand, 1 μM angiotensin III enhanced phosphatidyl inositol hydrolysis that was blocked by 1 μM losartan and 5 μM neomicin. PD 123319 (1 μM) did not affect ANG III-induced phosphatidyl inositol hydrolysis enhancement. 5. Our results confirm that angiotensin III acts as a modulator of noradrenergic transmission at the hypothalamic level through the AT1-phospholipase C pathway. This enhancement of hypothalamic noradrenergic activity suggests that angiotensin III may act as a central modulator of several biological processes regulated at this level by catecholamines, such as cardiovascular, endocrine, and autonomic functions as well as water and saline homeostasis.Fil: Rodriguez Campos, Muriel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Kadarian, Carina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Rodano, Valeria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Bianciotti, Liliana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Fernandez, Belisario Enrique. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Vatta, Marcelo Sergio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaSpringer/Plenum Publishers2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39271Rodriguez Campos, Muriel; Kadarian, Carina; Rodano, Valeria; Bianciotti, Liliana Graciela; Fernandez, Belisario Enrique; et al.; AT-1 receptor and phospholipase C are involved in angiotensin III modulation of hypothalamic noradrenergic transmission; Springer/Plenum Publishers; Cellular And Molecular Neurobiology.; 20; 6; 12-2000; 747-7620272-4340CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1023/A:1007059010571info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1023%2FA%3A1007059010571info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:38:17Zoai:ri.conicet.gov.ar:11336/39271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:38:17.505CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15.812429

Similares en LA Referencia