Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies
Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce b...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/131911 |
| Acceso en línea: | http://hdl.handle.net/11336/131911 |
| Access Level: | acceso abierto |
| Palabra clave: | BONE MARROW STROMAL CELLS BONE REGENERATION POLY-Ε-CAPROLACTONE POLYDIISOPROPYL FUMARATE RAW 264.7 MACROPHAGES STRONTIUM https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
| Sumario: | Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce both anabolic and anti-catabolic effects in bone. On the other hand, systemic increases in Sr 2+ levels can provoke adverse cardiovascular effects. In the present study we have developed a compatibilized blend of poly-ε-caprolactone (PCL) and polydiisopropyl fumarate (PDIPF) enriched with 1% or 5% Sr 2+ and evaluated the applicability of these biomaterials for BTE, both in vitro and in vivo. In vitro, whereas Blend + 5% Sr 2+ was pro-inflammatory and anti-osteogenic, Blend + 1% Sr 2+ released very low quantities of the cation; was not cytotoxic for cultured macrophages; and showed improved osteocompatibility when used as a substratum for primary cultures of bone marrow stromal cells. In vivo, implants with Blend + 1% Sr 2+ significantly increased bone tissue regeneration and improved fibrous bridging (vs. Blend alone), while neither inducing a local inflammatory response nor increased serum levels of Sr 2+ . These results indicate that our compatibilized blend of PCL-PDIPF enriched with 1% Sr 2+ could be useful for BTE. |
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