Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA

The present study describes the phenanthrene-degrading activity of <i>Sphingomonas paucimobilis</i> 20006FA and its ability to promote the bioavailability of phenanthrene. <i>S. paucimobilis</i> 20006FA was isolated from a phenanthrene-contaminated soil microcosm. The strain...

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Detalles Bibliográficos
Autores: Coppotelli, Bibiana Marina, Ibarrolaza, Agustín, Dias, Romina Laura, Del Panno, María Teresa, Berthe-Corti, Luise, Morelli, Irma Susana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/131864
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131864
Access Level:acceso abierto
Palabra clave:Ciencias Exactas
Polycyclic Aromatic Hydrocarbon
Phenanthrene
Mineral Medium
Sphingomonas
Sodium Salicylate
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spelling Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FACoppotelli, Bibiana MarinaIbarrolaza, AgustínDias, Romina LauraDel Panno, María TeresaBerthe-Corti, LuiseMorelli, Irma SusanaCiencias ExactasPolycyclic Aromatic HydrocarbonPhenanthreneMineral MediumSphingomonasSodium SalicylateThe present study describes the phenanthrene-degrading activity of <i>Sphingomonas paucimobilis</i> 20006FA and its ability to promote the bioavailability of phenanthrene. <i>S. paucimobilis</i> 20006FA was isolated from a phenanthrene-contaminated soil microcosm. The strain was able to grow in liquid mineral medium saturated with phenanthrene as the sole carbon source, showing high phenanthrene elimination (52.9% of the supplied phenanthrene within 20 days). The accumulation of 1-hydroxy-2-naphthoic acid and salicylic acid as major phenanthrene metabolites and the capacity of the strain to grow with sodium salicylate as the sole source of carbon and energy indicated that the <i>S. paucimobilis</i> 20006FA possesses a complete phenanthrene degradation pathway. However, under the studied conditions, the strain was able to mineralize only the 10% of the consumed phenanthrene. Investigations on the cell ability to promote bioavailability of phenanthrene showed that the <i>S. paucimobilis</i> strain 20006FA exhibited low cell hydrophobicity (0.13), a pronounced chemotaxis toward phenanthrene, and it was able to reduce the surface tension of mineral liquid medium supplemented with phenanthrene as sole carbon source. Scanning electron micrographs revealed that: (1) in suspension cultures, cells formed flocks and showed small vesicles on the cell surface and (2) cells were also able to adhere to phenanthrene crystals and to produce biofilms. Clearly, the strain seems to exhibit two different mechanisms to enhance phenanthrene bioavailability: biosurfactant production and adhesion to the phenanthrene crystals.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf266-276http://sedici.unlp.edu.ar/handle/10915/131864enginfo:eu-repo/semantics/altIdentifier/issn/1432-184Xinfo:eu-repo/semantics/altIdentifier/issn/0095-3628info:eu-repo/semantics/altIdentifier/doi/10.1007/s00248-009-9563-3info:eu-repo/semantics/altIdentifier/pmid/19609598info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T13:10:50Zoai:sedici.unlp.edu.ar:10915/131864Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 13:10:50.511SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
spellingShingle Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
Coppotelli, Bibiana Marina
Ciencias Exactas
Polycyclic Aromatic Hydrocarbon
Phenanthrene
Mineral Medium
Sphingomonas
Sodium Salicylate
title_short Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_full Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_fullStr Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_full_unstemmed Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_sort Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
dc.creator.none.fl_str_mv Coppotelli, Bibiana Marina
Ibarrolaza, Agustín
Dias, Romina Laura
Del Panno, María Teresa
Berthe-Corti, Luise
Morelli, Irma Susana
author Coppotelli, Bibiana Marina
author_facet Coppotelli, Bibiana Marina
Ibarrolaza, Agustín
Dias, Romina Laura
Del Panno, María Teresa
Berthe-Corti, Luise
Morelli, Irma Susana
author_role author
author2 Ibarrolaza, Agustín
Dias, Romina Laura
Del Panno, María Teresa
Berthe-Corti, Luise
Morelli, Irma Susana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Polycyclic Aromatic Hydrocarbon
Phenanthrene
Mineral Medium
Sphingomonas
Sodium Salicylate
topic Ciencias Exactas
Polycyclic Aromatic Hydrocarbon
Phenanthrene
Mineral Medium
Sphingomonas
Sodium Salicylate
description The present study describes the phenanthrene-degrading activity of <i>Sphingomonas paucimobilis</i> 20006FA and its ability to promote the bioavailability of phenanthrene. <i>S. paucimobilis</i> 20006FA was isolated from a phenanthrene-contaminated soil microcosm. The strain was able to grow in liquid mineral medium saturated with phenanthrene as the sole carbon source, showing high phenanthrene elimination (52.9% of the supplied phenanthrene within 20 days). The accumulation of 1-hydroxy-2-naphthoic acid and salicylic acid as major phenanthrene metabolites and the capacity of the strain to grow with sodium salicylate as the sole source of carbon and energy indicated that the <i>S. paucimobilis</i> 20006FA possesses a complete phenanthrene degradation pathway. However, under the studied conditions, the strain was able to mineralize only the 10% of the consumed phenanthrene. Investigations on the cell ability to promote bioavailability of phenanthrene showed that the <i>S. paucimobilis</i> strain 20006FA exhibited low cell hydrophobicity (0.13), a pronounced chemotaxis toward phenanthrene, and it was able to reduce the surface tension of mineral liquid medium supplemented with phenanthrene as sole carbon source. Scanning electron micrographs revealed that: (1) in suspension cultures, cells formed flocks and showed small vesicles on the cell surface and (2) cells were also able to adhere to phenanthrene crystals and to produce biofilms. Clearly, the strain seems to exhibit two different mechanisms to enhance phenanthrene bioavailability: biosurfactant production and adhesion to the phenanthrene crystals.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131864
url http://sedici.unlp.edu.ar/handle/10915/131864
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1432-184X
info:eu-repo/semantics/altIdentifier/issn/0095-3628
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00248-009-9563-3
info:eu-repo/semantics/altIdentifier/pmid/19609598
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
266-276
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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