Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization

Epithelial cadherin (E-cadherin) has been involved in several calcium-dependent cell-cell adhesion events; however, its participation in gamete interaction has not been fully investigated. Our results have demonstrated expression of E-cadherin mRNA in the human male reproductive tract showing higher...

Descripción completa

Detalles Bibliográficos
Autores: Marin Briggiler, Clara Isabel, Veiga, Maria Florencia, Matos, María Laura, González Echeverría, M. F., Furlong, L. I., Vazquez, Monica Hebe
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/25920
Acceso en línea:http://hdl.handle.net/11336/25920
Access Level:acceso abierto
Palabra clave:Human Spermatozoa
Epithelial Cadherin
Fertilization
Oocyte
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_cc974bfd7fa9d98c5ed2fc25dff2252f
oai_identifier_str oai:ri.conicet.gov.ar:11336/25920
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
title Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
spellingShingle Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
Marin Briggiler, Clara Isabel
Human Spermatozoa
Epithelial Cadherin
Fertilization
Oocyte
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
title_full Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
title_fullStr Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
title_full_unstemmed Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
title_sort Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization
dc.creator.none.fl_str_mv Marin Briggiler, Clara Isabel
Veiga, Maria Florencia
Matos, María Laura
González Echeverría, M. F.
Furlong, L. I.
Vazquez, Monica Hebe
author Marin Briggiler, Clara Isabel
author_facet Marin Briggiler, Clara Isabel
Veiga, Maria Florencia
Matos, María Laura
González Echeverría, M. F.
Furlong, L. I.
Vazquez, Monica Hebe
author_role author
author2 Veiga, Maria Florencia
Matos, María Laura
González Echeverría, M. F.
Furlong, L. I.
Vazquez, Monica Hebe
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Human Spermatozoa
Epithelial Cadherin
Fertilization
Oocyte
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Human Spermatozoa
Epithelial Cadherin
Fertilization
Oocyte
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Epithelial cadherin (E-cadherin) has been involved in several calcium-dependent cell-cell adhesion events; however, its participation in gamete interaction has not been fully investigated. Our results have demonstrated expression of E-cadherin mRNA in the human male reproductive tract showing higher levels in the caput, corpus and cauda epididymis than in the testis. The mature 122 kDa E-cadherin was detected in epididymal protein extracts and was localized in the epithelial cells from the three epididymal regions. Moreover, the 86 kDa E-cadherin ectodomain was found in cauda epididymal and seminal plasma. Western immunoblotting of human sperm protein extracts allowed the identification of four E-cadherin forms (122, 105, 97 and 86 kDa). The protein was localized in the acrosomal region of intact spermatozoa, remained associated with the head of acrosome-reacted cells and was also detected on the oocyte surface. A similar localization was determined for other proteins of the adhesion complex (beta-catenin and actin). Spermatozoa incubated with anti-E-cadherin antibodies showed impaired binding to homologous zona pellucida (ZP); in addition, presence of these antibodies inhibited the penetration of human spermatozoa to ZP-free hamster oocytes. The results presented here describe the expression of E-cadherin in the male reproductive tract and gametes and strongly suggest its involvement in adhesion events during human fertilization. The identification of proteins involved in gamete interaction will contribute to the understanding of the molecular basis of fertilization and help in the diagnosis and treatment of infertility.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25920
Marin Briggiler, Clara Isabel; Veiga, Maria Florencia; Matos, María Laura; González Echeverría, M. F.; Furlong, L. I.; et al.; Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization; Oxford University Press; Molecular Human Reproduction; 14; 10; 12-2008; 561-571
1360-9947
1460-2407
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25920
identifier_str_mv Marin Briggiler, Clara Isabel; Veiga, Maria Florencia; Matos, María Laura; González Echeverría, M. F.; Furlong, L. I.; et al.; Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization; Oxford University Press; Molecular Human Reproduction; 14; 10; 12-2008; 561-571
1360-9947
1460-2407
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gan053
info:eu-repo/semantics/altIdentifier/doi/10.1093/molehr/gan053
info:eu-repo/semantics/altIdentifier/pmid/18829448
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196226657189888
spelling Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilizationMarin Briggiler, Clara IsabelVeiga, Maria FlorenciaMatos, María LauraGonzález Echeverría, M. F.Furlong, L. I.Vazquez, Monica HebeHuman SpermatozoaEpithelial CadherinFertilizationOocytehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Epithelial cadherin (E-cadherin) has been involved in several calcium-dependent cell-cell adhesion events; however, its participation in gamete interaction has not been fully investigated. Our results have demonstrated expression of E-cadherin mRNA in the human male reproductive tract showing higher levels in the caput, corpus and cauda epididymis than in the testis. The mature 122 kDa E-cadherin was detected in epididymal protein extracts and was localized in the epithelial cells from the three epididymal regions. Moreover, the 86 kDa E-cadherin ectodomain was found in cauda epididymal and seminal plasma. Western immunoblotting of human sperm protein extracts allowed the identification of four E-cadherin forms (122, 105, 97 and 86 kDa). The protein was localized in the acrosomal region of intact spermatozoa, remained associated with the head of acrosome-reacted cells and was also detected on the oocyte surface. A similar localization was determined for other proteins of the adhesion complex (beta-catenin and actin). Spermatozoa incubated with anti-E-cadherin antibodies showed impaired binding to homologous zona pellucida (ZP); in addition, presence of these antibodies inhibited the penetration of human spermatozoa to ZP-free hamster oocytes. The results presented here describe the expression of E-cadherin in the male reproductive tract and gametes and strongly suggest its involvement in adhesion events during human fertilization. The identification of proteins involved in gamete interaction will contribute to the understanding of the molecular basis of fertilization and help in the diagnosis and treatment of infertility.Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; ArgentinaFil: Veiga, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; ArgentinaFil: Matos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; ArgentinaFil: González Echeverría, M. F.. Fertilab; ArgentinaFil: Furlong, L. I.. Instituto Hospital del Mar de Investigaciones Medicas; EspañaFil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires; ArgentinaOxford University Press2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25920Marin Briggiler, Clara Isabel; Veiga, Maria Florencia; Matos, María Laura; González Echeverría, M. F.; Furlong, L. I.; et al.; Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization; Oxford University Press; Molecular Human Reproduction; 14; 10; 12-2008; 561-5711360-99471460-2407CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gan053info:eu-repo/semantics/altIdentifier/doi/10.1093/molehr/gan053info:eu-repo/semantics/altIdentifier/pmid/18829448info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:19:33Zoai:ri.conicet.gov.ar:11336/25920instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:19:34.036CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15.811543

Similares en LA Referencia