Phytochemical and biological study of phenolic fraction from Geoffroea decorticans bark

Abstract: The stem bark of Geoffroea decorticans (Gill.ex Hook. et Arn.) Burk. (Fabaceae) was used medicinally to cure several skin affections; however, previous phytochemical and biological antecedents that supported its vulnerary property were not found yet. A purified methanolic fraction from G....

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Detalles Bibliográficos
Autores: Pastoriza, Ana Cristina, Sgariglia, Melina Araceli, Soberon, Jose Rodolfo, Sampietro, Diego Alejandro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/159790
Acceso en línea:http://hdl.handle.net/11336/159790
Access Level:acceso abierto
Palabra clave:GEOFFROEA DECORTICANS BARK
PHENOLIC FRACTION
PHYTOCHEMICAL ANALYSES
ANTIBACTERIAL
CYTOTOXICITY
https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
Descripción
Sumario:Abstract: The stem bark of Geoffroea decorticans (Gill.ex Hook. et Arn.) Burk. (Fabaceae) was used medicinally to cure several skin affections; however, previous phytochemical and biological antecedents that supported its vulnerary property were not found yet. A purified methanolic fraction from G. decorticans bark (PFGB), was derivatized by silylation for GC/MS analysis, and complementary analyses by two-dimensional TLC and UV-Vis spectroscopy against detected standards compounds. Nine phenolic compounds were characterized as gallic acid, quercetin, kaempferol, apigenin, protocatechuic acid, taxifolin, 4-Hydroxybenzoic acid; among these two methoxy-flavonoids. PFGB showed significant antibacterial activity (MICs = 125 μg/mL) against Staphylococcus aureus (25923) and Enterococcus faecalis (29212) ATCC strains. Cytotoxic effects on human lymphocytes activated or not by LPS were not observed up to 100 µg/mL, by MTT colorimetric assay. The results obtained through the ethnopharmacological approach of this work contribute to the scientific validation of the vulnerary medicinal use of G. decorticans.