Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters

Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage....

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Detalles Bibliográficos
Autores: Da Re, Cristina Verónica, Papinutti, Víctor Leandro, Forchiassin, Flavia, Levin, Laura Noemí
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/26928
Acceso en línea:http://hdl.handle.net/11336/26928
Access Level:acceso abierto
Palabra clave:Softwood Kraft Pulp
Trames Trogii
Laccase
Mn Peroxidase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
title Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
spellingShingle Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
Da Re, Cristina Verónica
Softwood Kraft Pulp
Trames Trogii
Laccase
Mn Peroxidase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
title_full Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
title_fullStr Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
title_full_unstemmed Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
title_sort Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters
dc.creator.none.fl_str_mv Da Re, Cristina Verónica
Papinutti, Víctor Leandro
Forchiassin, Flavia
Levin, Laura Noemí
author Da Re, Cristina Verónica
author_facet Da Re, Cristina Verónica
Papinutti, Víctor Leandro
Forchiassin, Flavia
Levin, Laura Noemí
author_role author
author2 Papinutti, Víctor Leandro
Forchiassin, Flavia
Levin, Laura Noemí
author2_role author
author
author
dc.subject.none.fl_str_mv Softwood Kraft Pulp
Trames Trogii
Laccase
Mn Peroxidase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Softwood Kraft Pulp
Trames Trogii
Laccase
Mn Peroxidase
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26928
Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Forchiassin, Flavia; Levin, Laura Noemí; Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters; Elsevier Science Inc; Enzyme And Microbial Technology; 46; 3-4; 28-12-2009; 281-286
0141-0229
1879-0909
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26928
identifier_str_mv Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Forchiassin, Flavia; Levin, Laura Noemí; Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters; Elsevier Science Inc; Enzyme And Microbial Technology; 46; 3-4; 28-12-2009; 281-286
0141-0229
1879-0909
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141022909002828
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2009.12.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196430672330752
spelling Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parametersDa Re, Cristina VerónicaPapinutti, Víctor LeandroForchiassin, FlaviaLevin, Laura NoemíSoftwood Kraft PulpTrames TrogiiLaccaseMn Peroxidasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme.Fil: Da Re, Cristina Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Forchiassin, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaElsevier Science Inc2009-12-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26928Da Re, Cristina Verónica; Papinutti, Víctor Leandro; Forchiassin, Flavia; Levin, Laura Noemí; Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage: application of Doehlert experimental design to evaluate process parameters; Elsevier Science Inc; Enzyme And Microbial Technology; 46; 3-4; 28-12-2009; 281-2860141-02291879-0909CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141022909002828info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2009.12.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:27:31Zoai:ri.conicet.gov.ar:11336/26928instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:27:32.198CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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