The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation

The aim of this study was to examine the effect of varying intracellular reactive oxygen species (ROS) levels during oocyte in vitro maturation with enzymatic ROS production systems (xanthine + xanthine oxidase or xanthine + xanthine oxidase + catalase), scavenger systems (catalase or superoxide dis...

Descripción completa

Detalles Bibliográficos
Autores: Alvarez, Gabriel Martín, Morado, Sergio Adrián, Soto, Mariela Patricia, Dalvit, Gabriel Carlos, Cetica, Pablo Daniel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/46695
Acceso en línea:http://hdl.handle.net/11336/46695
Access Level:acceso abierto
Palabra clave:Reactive Oxygen Species
In Vitro Maturation
Oocytes
Porcine
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
id AR_cb52f56df416d25b024ee8b24e201623
oai_identifier_str oai:ri.conicet.gov.ar:11336/46695
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
title The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
spellingShingle The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
Alvarez, Gabriel Martín
Reactive Oxygen Species
In Vitro Maturation
Oocytes
Porcine
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
title_short The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
title_full The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
title_fullStr The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
title_full_unstemmed The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
title_sort The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation
dc.creator.none.fl_str_mv Alvarez, Gabriel Martín
Morado, Sergio Adrián
Soto, Mariela Patricia
Dalvit, Gabriel Carlos
Cetica, Pablo Daniel
author Alvarez, Gabriel Martín
author_facet Alvarez, Gabriel Martín
Morado, Sergio Adrián
Soto, Mariela Patricia
Dalvit, Gabriel Carlos
Cetica, Pablo Daniel
author_role author
author2 Morado, Sergio Adrián
Soto, Mariela Patricia
Dalvit, Gabriel Carlos
Cetica, Pablo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Reactive Oxygen Species
In Vitro Maturation
Oocytes
Porcine
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
topic Reactive Oxygen Species
In Vitro Maturation
Oocytes
Porcine
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
description The aim of this study was to examine the effect of varying intracellular reactive oxygen species (ROS) levels during oocyte in vitro maturation with enzymatic ROS production systems (xanthine + xanthine oxidase or xanthine + xanthine oxidase + catalase), scavenger systems (catalase or superoxide dismutase + catalase) or cysteine on porcine oocyte maturation. Oocyte ROS levels showed an increase when H2O2 or O2∙(-) production systems were added to the culture medium (p < 0.05). On the other hand, the presence of ROS scavengers in the maturation medium did not modify oocyte ROS levels compared with the control after 48 h of maturation, but the addition of cysteine induced a decrease in oocyte ROS levels (p < 0.05). The ROS production systems used in this work did not modified the percentage of oocyte nuclear maturation, but increased the decondensation of sperm head (p < 0.05) and decreased the pronuclear formation (p < 0.05). In turn, the addition of O2∙(-) and H2O2 scavenging systems during in vitro maturation did not modify the percentage of oocytes reaching metaphase II nor the oocytes with decondensed sperm head or pronuclei after fertilization. However, both parameters increased in the presence of cysteine (p < 0.05). The exogenous generation of O2∙(-) and H2O2 during oocyte in vitro maturation would not affect nuclear maturation or later sperm penetration, but most of the spermatozoa cannot progress to form the pronuclei after fusion with the oocyte. The decrease in endogenous ROS levels by the addition of cysteine would improve pronuclear formation after sperm penetration.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46695
Alvarez, Gabriel Martín; Morado, Sergio Adrián; Soto, Mariela Patricia; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel; The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 2; 4-2015; 200-205
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46695
identifier_str_mv Alvarez, Gabriel Martín; Morado, Sergio Adrián; Soto, Mariela Patricia; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel; The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 2; 4-2015; 200-205
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12469
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12469
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194852187963392
spelling The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro MaturationAlvarez, Gabriel MartínMorado, Sergio AdriánSoto, Mariela PatriciaDalvit, Gabriel CarlosCetica, Pablo DanielReactive Oxygen SpeciesIn Vitro MaturationOocytesPorcinehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to examine the effect of varying intracellular reactive oxygen species (ROS) levels during oocyte in vitro maturation with enzymatic ROS production systems (xanthine + xanthine oxidase or xanthine + xanthine oxidase + catalase), scavenger systems (catalase or superoxide dismutase + catalase) or cysteine on porcine oocyte maturation. Oocyte ROS levels showed an increase when H2O2 or O2∙(-) production systems were added to the culture medium (p < 0.05). On the other hand, the presence of ROS scavengers in the maturation medium did not modify oocyte ROS levels compared with the control after 48 h of maturation, but the addition of cysteine induced a decrease in oocyte ROS levels (p < 0.05). The ROS production systems used in this work did not modified the percentage of oocyte nuclear maturation, but increased the decondensation of sperm head (p < 0.05) and decreased the pronuclear formation (p < 0.05). In turn, the addition of O2∙(-) and H2O2 scavenging systems during in vitro maturation did not modify the percentage of oocytes reaching metaphase II nor the oocytes with decondensed sperm head or pronuclei after fertilization. However, both parameters increased in the presence of cysteine (p < 0.05). The exogenous generation of O2∙(-) and H2O2 during oocyte in vitro maturation would not affect nuclear maturation or later sperm penetration, but most of the spermatozoa cannot progress to form the pronuclei after fusion with the oocyte. The decrease in endogenous ROS levels by the addition of cysteine would improve pronuclear formation after sperm penetration.Fil: Alvarez, Gabriel Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Morado, Sergio Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Soto, Mariela Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Dalvit, Gabriel Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaWiley Blackwell Publishing, Inc2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46695Alvarez, Gabriel Martín; Morado, Sergio Adrián; Soto, Mariela Patricia; Dalvit, Gabriel Carlos; Cetica, Pablo Daniel; The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 50; 2; 4-2015; 200-2050936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12469info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.12469info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:37:54Zoai:ri.conicet.gov.ar:11336/46695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:37:54.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,812429

Similares en LA Referencia