Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy

(1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that en...

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Detalles Bibliográficos
Autores: Schuster, Christina, Baró Robaina, Yamilé, Ben Gharsa, Haifa, Bobushova, Saikal, Manfrino, Romina Guadalupe, Gutierrez, Alejandra Concepción, Lopez Lastra, Claudia Cristina, Doolotkeldieva, Tinatin, Leclerque, Andreas
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/227256
Acceso en línea:http://hdl.handle.net/11336/227256
Access Level:acceso abierto
Palabra clave:DIAGNOSTIC PCR
METARHIZIUM ANISOPLIAE
METARHIZIUM BRUNNEUM
METARHIZIUM ROBERTSII
METARHIZUM PINGHAENSE
PARB CLADE
RIBOSOMAL INTERGENIC SPACER (RIGS)
SINGLE-MARKER PHYLOGENY
SPECIES DELINEATION
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
Descripción
Sumario:(1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.

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