Seed germination and viability of Wyoming sagebrush in northern Nevada

Seed size and germination behavior affect performance of early seedlings. The purpose of this study was to investigate relationships between seed size and germination percentage, germination rate, time course of germination and seed viability in Wyoming big sagebrush (Artemisia tridentatassp. wyomin...

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Detalles Bibliográficos
Autores: Busso, Carlos Alberto, Mazzola, Monica Beatriz, Perryman, Barry L.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/34326
Acceso en línea:http://hdl.handle.net/11336/34326
Access Level:acceso abierto
Palabra clave:Seed Weight Versus Seed Germination and Viability
Artemisia tridentata ssp. wyomingensis
Rangeland Restoration
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
Descripción
Sumario:Seed size and germination behavior affect performance of early seedlings. The purpose of this study was to investigate relationships between seed size and germination percentage, germination rate, time course of germination and seed viability in Wyoming big sagebrush (Artemisia tridentatassp. wyomingensis Beetle and Young). Working hypotheses were: 1) for single seeds,germination percentages and rates are positively related to seed weight; and 2) some TTC-unstained seeds are not unviable, butdormant. Seed collection was conducted at Battle Mountain and Eden Valley sites in northern Nevada during November 2002 and 2003. Individual sagebrush seeds were placed into weight classes,using 0.05mg·seed-1 increments, ranging from ≤0.15 to>0.40mg·seed-1. Seeds were incubated in darkness at 15ºC, seedlings with radicles ≥1.0mm were removed daily during the first 10days, and every other day thereafter until day 32. The study terminated on day 37. Triphenyl tetrazolium chloride (TTC) test wasused to determine viability of ungerminated seeds, and Evan’s blue to sort out ungerminated seeds into either dead or dormant categories. Results supported both hypotheses. Maximum germination at both sites, years and all seed weight ranges was reachedin 5 to 6 days after imbibition. This suggests that moist soil forseveral consecutive days would help provide optimum field germi-nation in this species. Use of the vital stain Evan’s blue demon-strated that, albeit in a small percentage (≤5%), some TTC-un-stained seeds were not dead but only dormant with germination potential given appropriate conditions. When sagebrush is used forrestoration, relatively heavy seeds should be used because they have the greatest germination potential.