Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters

Mercury is a widespread pollutant. Mercuric ions uptake into tubular cells is supported by the Organic anion transporter 1 (Oat1) and 3 (Oat3) and its elimination into urine is through the Multidrug resistance-associated protein 2 (Mrp2). We investigated the effect of recombinant human erythropoieti...

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Detalhes bibliográficos
Autores: Hazelhoff, Maria Herminia, Torres, Adriana Monica
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/155501
Acesso em linha:http://hdl.handle.net/11336/155501
Access Level:acceso abierto
Palavra-chave:ERYTHR0POIETIN
NEPHROTOXICITY
MERCURY
MEMBRANE TRANSPORTERS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
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network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transportersHazelhoff, Maria HerminiaTorres, Adriana MonicaERYTHR0POIETINNEPHROTOXICITYMERCURYMEMBRANE TRANSPORTERShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Mercury is a widespread pollutant. Mercuric ions uptake into tubular cells is supported by the Organic anion transporter 1 (Oat1) and 3 (Oat3) and its elimination into urine is through the Multidrug resistance-associated protein 2 (Mrp2). We investigated the effect of recombinant human erythropoietin (Epo) on renal function and on renal expression of Oat1, Oat3, and Mrp2 in a model of mercuric chloride (HgCl2)- induced renal damage. Four experimental groups of adult male Wistar rats were used: Control, Epo, HgCl2, and Epo+HgCl2. Epo (3000 IU/kg, b.w., i.p) was administered 24 h before HgCl2 (4 mg/kg, b.w., i.p). Experiments were performed 18 h after the HgCl2 dose. Parameters of renal function and structure were evaluated. The protein expression of Oat1, Oat3 and Mrp2 in renal tissue was assessed by immunoblotting techniques. Mercury levels were determined by cold vapour atomic absorption spectrometry. Pretreatment with Epo ameliorated the HgCl2-induced tubular injury as assessed by histopathology and urinary biomarkers. Immunoblotting showed that pretreatment with Epo regulated the renal expression of mercury transporters in a way to decrease mercury content in the kidney. Epo pretreatment ameliorates HgCl2-induced renal tubular injury by modulation of mercury transporters expression in the kidneys.Fil: Hazelhoff, Maria Herminia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Ciencias Fisiológicas. Área Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Torres, Adriana Monica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Ciencias Fisiológicas. Área Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaSAGE Publications2021-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155501Hazelhoff, Maria Herminia; Torres, Adriana Monica; Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters; SAGE Publications; Human and Experimental Toxicoloxy; 40; 3; 10-3-2021; 515-5250960-32711477-0903CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/0960327120958109info:eu-repo/semantics/altIdentifier/doi/10.1177%2F0960327120958109info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:22:01Zoai:ri.conicet.gov.ar:11336/155501instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:22:01.925CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
title Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
spellingShingle Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
Hazelhoff, Maria Herminia
ERYTHR0POIETIN
NEPHROTOXICITY
MERCURY
MEMBRANE TRANSPORTERS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
title_short Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
title_full Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
title_fullStr Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
title_full_unstemmed Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
title_sort Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters
dc.creator.none.fl_str_mv Hazelhoff, Maria Herminia
Torres, Adriana Monica
author Hazelhoff, Maria Herminia
author_facet Hazelhoff, Maria Herminia
Torres, Adriana Monica
author_role author
author2 Torres, Adriana Monica
author2_role author
dc.subject.none.fl_str_mv ERYTHR0POIETIN
NEPHROTOXICITY
MERCURY
MEMBRANE TRANSPORTERS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
topic ERYTHR0POIETIN
NEPHROTOXICITY
MERCURY
MEMBRANE TRANSPORTERS
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
description Mercury is a widespread pollutant. Mercuric ions uptake into tubular cells is supported by the Organic anion transporter 1 (Oat1) and 3 (Oat3) and its elimination into urine is through the Multidrug resistance-associated protein 2 (Mrp2). We investigated the effect of recombinant human erythropoietin (Epo) on renal function and on renal expression of Oat1, Oat3, and Mrp2 in a model of mercuric chloride (HgCl2)- induced renal damage. Four experimental groups of adult male Wistar rats were used: Control, Epo, HgCl2, and Epo+HgCl2. Epo (3000 IU/kg, b.w., i.p) was administered 24 h before HgCl2 (4 mg/kg, b.w., i.p). Experiments were performed 18 h after the HgCl2 dose. Parameters of renal function and structure were evaluated. The protein expression of Oat1, Oat3 and Mrp2 in renal tissue was assessed by immunoblotting techniques. Mercury levels were determined by cold vapour atomic absorption spectrometry. Pretreatment with Epo ameliorated the HgCl2-induced tubular injury as assessed by histopathology and urinary biomarkers. Immunoblotting showed that pretreatment with Epo regulated the renal expression of mercury transporters in a way to decrease mercury content in the kidney. Epo pretreatment ameliorates HgCl2-induced renal tubular injury by modulation of mercury transporters expression in the kidneys.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155501
Hazelhoff, Maria Herminia; Torres, Adriana Monica; Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters; SAGE Publications; Human and Experimental Toxicoloxy; 40; 3; 10-3-2021; 515-525
0960-3271
1477-0903
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155501
identifier_str_mv Hazelhoff, Maria Herminia; Torres, Adriana Monica; Effect of erythropoietin on mercury-induced nephrotoxicity: Role of membrane transporters; SAGE Publications; Human and Experimental Toxicoloxy; 40; 3; 10-3-2021; 515-525
0960-3271
1477-0903
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/0960327120958109
info:eu-repo/semantics/altIdentifier/doi/10.1177%2F0960327120958109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,811543

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