The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress
Confocal microscopy images revealed that the tetratricopeptide repeat motif (TPR) domain immunophilin FKBP51 shows colocalization with the specific mitochondrial marker Mito-Tracker. Signal specificity was tested with different antibodies and by FKBP51 knockdown. This unexpected subcellular localiza...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Argentina |
| Institución: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositorio: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglés |
| OAI Identifier: | paperaa:paper_00219258_v286_n34_p30152_Gallo |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219258_v286_n34_p30152_Gallo |
| Access Level: | acceso abierto |
| Palabra clave: | Antiapoptotic Biochemical fractionation Co-localization studies Colocalization Glucocorticoid receptor Heat-shock protein Immunophilins Mitochondrial marker Mitochondrial protein Over-expression Stress response Subcellular localizations Tetratricopeptide repeat motif Confocal microscopy Proteins Mitochondria chaperone fk 506 binding protein 51 glucocorticoid receptor heat shock protein 70 heat shock protein 90 immunophilin mitochondrial protein unclassified drug article cell nucleus cell protection cell strain 3T3 cellular distribution cellular stress response complex formation controlled study electron microscopy gene silencing mitochondrion oxidative stress priority journal protein analysis protein domain protein expression protein function protein localization protein transport tetratricopeptide repeat 3T3-L1 Cells Active Transport, Cell Nucleus Animals Cell Nucleus HSP70 Heat-Shock Proteins HSP90 Heat-Shock Proteins Humans Mice Mitochondrial Proteins Mutation Oxidative Stress Protein Structure, Tertiary Receptors, Glucocorticoid Tacrolimus Binding Proteins Vasconcellea candicans |
| Sumario: | Confocal microscopy images revealed that the tetratricopeptide repeat motif (TPR) domain immunophilin FKBP51 shows colocalization with the specific mitochondrial marker Mito-Tracker. Signal specificity was tested with different antibodies and by FKBP51 knockdown. This unexpected subcellular localization of FKBP51 was confirmed by colocalization studies with other mitochondrial proteins, biochemical fractionation, and electron microscopy imaging. Interestingly, FKBP51 forms complexes in mitochondria with the glucocorticoid receptor and the Hsp90/Hsp70-based chaperone heterocomplex. Although Hsp90 inhibitors favor FKBP51 translocation from mitochondria to the nucleus in a reversible manner, TPR domain-deficient mutants of FKBP51 are constitutively nuclear and fully excluded from mitochondria, suggesting that a functional TPR domain is required for its mitochondrial localization. FKBP51 overexpression protects cells against oxidative stress, whereas FKBP51 knockdown makes them more sensitive to injury. In summary, this is the first demonstration that FKBP51 is a major mitochondrial factor that undergoes nuclear-mitochondrial shuttling, an observation that may be related to antiapoptotic mechanisms triggered during the stress response. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. |
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