Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses

In order to detect serum antibodies against clinically important Old and New World hantaviruses simultaneously, multiparametric indirect immunofluorescence assays (IFAs) based on biochip mosaics were developed. Each of the mosaic substrates consisted of cells infected with one of the virus types Han...

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Detalhes bibliográficos
Autores: Lederer, Sabine, Lattwein, Erik, Hanke, Merle, Sonnenberg, Karen, Stoecker, Winfried, Lundkvist, Åke, Vaheri, Antti, Vapalahti, Olli, Chan, Paul K. S., Feldmann, Heinz, Dick, Daryl, Schmidt Chanasit, Jonas, Padula, Paula Julieta, Vial, Pablo A., Panculescu Gatej, Raluca, Ceianu, Cornelia, Heyman, Paul, Avšič Županc, Tatjana, Niedrig, Matthias
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/11026
Acesso em linha:http://hdl.handle.net/11336/11026
Access Level:acceso abierto
Palavra-chave:Monoclonals
hantavirus
ELISA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11026
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
title Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
spellingShingle Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
Lederer, Sabine
Monoclonals
hantavirus
ELISA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
title_full Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
title_fullStr Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
title_full_unstemmed Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
title_sort Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
dc.creator.none.fl_str_mv Lederer, Sabine
Lattwein, Erik
Hanke, Merle
Sonnenberg, Karen
Stoecker, Winfried
Lundkvist, Åke
Vaheri, Antti
Vapalahti, Olli
Chan, Paul K. S.
Feldmann, Heinz
Dick, Daryl
Schmidt Chanasit, Jonas
Padula, Paula Julieta
Vial, Pablo A.
Panculescu Gatej, Raluca
Ceianu, Cornelia
Heyman, Paul
Avšič Županc, Tatjana
Niedrig, Matthias
author Lederer, Sabine
author_facet Lederer, Sabine
Lattwein, Erik
Hanke, Merle
Sonnenberg, Karen
Stoecker, Winfried
Lundkvist, Åke
Vaheri, Antti
Vapalahti, Olli
Chan, Paul K. S.
Feldmann, Heinz
Dick, Daryl
Schmidt Chanasit, Jonas
Padula, Paula Julieta
Vial, Pablo A.
Panculescu Gatej, Raluca
Ceianu, Cornelia
Heyman, Paul
Avšič Županc, Tatjana
Niedrig, Matthias
author_role author
author2 Lattwein, Erik
Hanke, Merle
Sonnenberg, Karen
Stoecker, Winfried
Lundkvist, Åke
Vaheri, Antti
Vapalahti, Olli
Chan, Paul K. S.
Feldmann, Heinz
Dick, Daryl
Schmidt Chanasit, Jonas
Padula, Paula Julieta
Vial, Pablo A.
Panculescu Gatej, Raluca
Ceianu, Cornelia
Heyman, Paul
Avšič Županc, Tatjana
Niedrig, Matthias
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Monoclonals
hantavirus
ELISA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Monoclonals
hantavirus
ELISA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description In order to detect serum antibodies against clinically important Old and New World hantaviruses simultaneously, multiparametric indirect immunofluorescence assays (IFAs) based on biochip mosaics were developed. Each of the mosaic substrates consisted of cells infected with one of the virus types Hantaan (HTNV), Puumala (PUUV), Seoul (SEOV), Saaremaa (SAAV), Dobrava (DOBV), Sin Nombre (SNV) or Andes (ANDV). For assay evaluation, serum IgG and IgM antibodies were analyzed using 184 laboratory-confirmed hantavirus-positive sera collected at six diagnostic centers from patients actively or previously infected with the following hantavirus serotypes: PUUV (Finland, n = 97); SEOV (China, n = 5); DOBV (Romania, n = 7); SNV (Canada, n = 23); ANDV (Argentina and Chile, n = 52). The control panel comprised 89 sera from healthy blood donors. According to the reference tests, all 184 patient samples were seropositive for hantavirus-specific IgG (n = 177; 96%) and/or IgM (n = 131; 72%), while all control samples were tested negative. In the multiparametric IFA applied in this study, 183 (99%) of the patient sera were IgG and 131 (71%) IgM positive (accordance with the reference tests: IgG, 96%; IgM, 93%). Overall IFA sensitivity for combined IgG and IgM analysis amounted to 100% for all serotypes, except for SNV (96%). Of the 89 control sera, 2 (2%) showed IgG reactivity against the HTNV substrate, but not against any other hantavirus. Due to the high cross-reactivity of hantaviral nucleocapsid proteins, endpoint titrations were conducted, allowing serotype determination in .90% of PUUV- and ANDV-infected patients. Thus, multiparametric IFA enables highly sensitive and specific serological diagnosis of hantavirus infections and can be used to differentiate PUUV and ANDV infection from infections with Murinae-borne hantaviruses (e.g. DOBV and SEOV).
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11026
Lederer, Sabine; Lattwein, Erik; Hanke, Merle; Sonnenberg, Karen; Stoecker, Winfried; et al.; Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses; Public Library Of Science; Neglected Tropical Diseases; 7; 4-2013; 1-9
1935-2735
url http://hdl.handle.net/11336/11026
identifier_str_mv Lederer, Sabine; Lattwein, Erik; Hanke, Merle; Sonnenberg, Karen; Stoecker, Winfried; et al.; Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses; Public Library Of Science; Neglected Tropical Diseases; 7; 4-2013; 1-9
1935-2735
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002157
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593524/
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002157
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195466446929920
spelling Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World HantavirusesLederer, SabineLattwein, ErikHanke, MerleSonnenberg, KarenStoecker, WinfriedLundkvist, ÅkeVaheri, AnttiVapalahti, OlliChan, Paul K. S.Feldmann, HeinzDick, DarylSchmidt Chanasit, JonasPadula, Paula JulietaVial, Pablo A.Panculescu Gatej, RalucaCeianu, CorneliaHeyman, PaulAvšič Županc, TatjanaNiedrig, MatthiasMonoclonalshantavirusELISAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In order to detect serum antibodies against clinically important Old and New World hantaviruses simultaneously, multiparametric indirect immunofluorescence assays (IFAs) based on biochip mosaics were developed. Each of the mosaic substrates consisted of cells infected with one of the virus types Hantaan (HTNV), Puumala (PUUV), Seoul (SEOV), Saaremaa (SAAV), Dobrava (DOBV), Sin Nombre (SNV) or Andes (ANDV). For assay evaluation, serum IgG and IgM antibodies were analyzed using 184 laboratory-confirmed hantavirus-positive sera collected at six diagnostic centers from patients actively or previously infected with the following hantavirus serotypes: PUUV (Finland, n = 97); SEOV (China, n = 5); DOBV (Romania, n = 7); SNV (Canada, n = 23); ANDV (Argentina and Chile, n = 52). The control panel comprised 89 sera from healthy blood donors. According to the reference tests, all 184 patient samples were seropositive for hantavirus-specific IgG (n = 177; 96%) and/or IgM (n = 131; 72%), while all control samples were tested negative. In the multiparametric IFA applied in this study, 183 (99%) of the patient sera were IgG and 131 (71%) IgM positive (accordance with the reference tests: IgG, 96%; IgM, 93%). Overall IFA sensitivity for combined IgG and IgM analysis amounted to 100% for all serotypes, except for SNV (96%). Of the 89 control sera, 2 (2%) showed IgG reactivity against the HTNV substrate, but not against any other hantavirus. Due to the high cross-reactivity of hantaviral nucleocapsid proteins, endpoint titrations were conducted, allowing serotype determination in .90% of PUUV- and ANDV-infected patients. Thus, multiparametric IFA enables highly sensitive and specific serological diagnosis of hantavirus infections and can be used to differentiate PUUV and ANDV infection from infections with Murinae-borne hantaviruses (e.g. DOBV and SEOV).Fil: Lederer, Sabine. EUROIMMUN Medizinische Labordiagnostika; AlemaniaFil: Lattwein, Erik. EUROIMMUN Medizinische Labordiagnostika; AlemaniaFil: Hanke, Merle. EUROIMMUN Medizinische Labordiagnostika; AlemaniaFil: Sonnenberg, Karen. EUROIMMUN Medizinische Labordiagnostika; AlemaniaFil: Stoecker, Winfried. EUROIMMUN Medizinische Labordiagnostika; AlemaniaFil: Lundkvist, Åke. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Vaheri, Antti. University of Helsinki; FinlandiaFil: Vapalahti, Olli. University of Helsinki; FinlandiaFil: Chan, Paul K. S.. Chinese University of Hong Kong; República de ChinaFil: Feldmann, Heinz. Canadian Science Centre for Human and Animal Health. Public Health Agency of Canada; CanadáFil: Dick, Daryl. Canadian Science Centre for Human and Animal Health. Public Health Agency of Canada; CanadáFil: Schmidt Chanasit, Jonas. Bernhard Nocht Institute for Tropical Medicine; AlemaniaFil: Padula, Paula Julieta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vial, Pablo A.. Universidad del Desarrollo; ChileFil: Panculescu Gatej, Raluca. CANTACUZINO National Institute for Research and Development in Microbiology and Immunology; RumaniaFil: Ceianu, Cornelia. CANTACUZINO National Institute for Research and Development in Microbiology and Immunology; RumaniaFil: Heyman, Paul. Queen Astrid Military Hospital; BélgicaFil: Avšič Županc, Tatjana. Institute of Microbiology and Immunology; EsloveniaFil: Niedrig, Matthias. Robert Koch Institute; AlemaniaPublic Library Of Science2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11026Lederer, Sabine; Lattwein, Erik; Hanke, Merle; Sonnenberg, Karen; Stoecker, Winfried; et al.; Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses; Public Library Of Science; Neglected Tropical Diseases; 7; 4-2013; 1-91935-2735enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002157info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593524/info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002157info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:53:20Zoai:ri.conicet.gov.ar:11336/11026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:53:20.77CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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