Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial

Single-molecule localization microscopy (SMLM) has become an essential tool to investigate phenomena at the nanoscale. Among the different SMLM approaches, methods that interrogate the molecular position with an intensity minimum, such as minimal emission fluxes (MINFLUX) or the more recent raster s...

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Detalles Bibliográficos
Autores: Lopez, Lucía Fernanda, Masullo, Luciano Andrés, Szalai, Alan Marcelo, Edorna, Florencia Belen, Choque, Florencia Daniela, Caprile, Fernando, Stefani, Fernando Daniel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/228958
Acceso en línea:http://hdl.handle.net/11336/228958
Access Level:acceso abierto
Palabra clave:Fluorescence
Single Molecule Localization Microscopy
MINFLUX
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
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spelling Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorialLopez, Lucía FernandaMasullo, Luciano AndrésSzalai, Alan MarceloEdorna, Florencia BelenChoque, Florencia DanielaCaprile, FernandoStefani, Fernando DanielFluorescenceSingle Molecule Localization MicroscopyMINFLUXhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Single-molecule localization microscopy (SMLM) has become an essential tool to investigate phenomena at the nanoscale. Among the different SMLM approaches, methods that interrogate the molecular position with an intensity minimum, such as minimal emission fluxes (MINFLUX) or the more recent raster scanning a minimum of light (RASTMIN), stand out for reaching true molecular resolution. To implement these methods, the phase of the excitation beam needs to be modulated to obtain a focus with a central minimum, i.e., a so-called toroidal or doughnut-shaped focus. In this tutorial,we explain the basis and experimental tricks to generate and optimize such beams, particularly in raster-scanning microscopes.Fil: Lopez, Lucía Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Masullo, Luciano Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Szalai, Alan Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Edorna, Florencia Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Choque, Florencia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Caprile, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Stefani, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaOptical Society of America2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228958Lopez, Lucía Fernanda; Masullo, Luciano Andrés; Szalai, Alan Marcelo; Edorna, Florencia Belen; Choque, Florencia Daniela; et al.; Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial; Optical Society of America; Journal of the Optical Society of America B-Optical Physics; 40; 4; 4-2023; 103-1100740-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://opg.optica.org/josab/fulltext.cfm?uri=josab-40-4-C103&id=528677info:eu-repo/semantics/altIdentifier/doi/10.1364/JOSAB.482413info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:51:23Zoai:ri.conicet.gov.ar:11336/228958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:51:23.754CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
title Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
spellingShingle Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
Lopez, Lucía Fernanda
Fluorescence
Single Molecule Localization Microscopy
MINFLUX
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
title_short Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
title_full Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
title_fullStr Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
title_full_unstemmed Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
title_sort Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial
dc.creator.none.fl_str_mv Lopez, Lucía Fernanda
Masullo, Luciano Andrés
Szalai, Alan Marcelo
Edorna, Florencia Belen
Choque, Florencia Daniela
Caprile, Fernando
Stefani, Fernando Daniel
author Lopez, Lucía Fernanda
author_facet Lopez, Lucía Fernanda
Masullo, Luciano Andrés
Szalai, Alan Marcelo
Edorna, Florencia Belen
Choque, Florencia Daniela
Caprile, Fernando
Stefani, Fernando Daniel
author_role author
author2 Masullo, Luciano Andrés
Szalai, Alan Marcelo
Edorna, Florencia Belen
Choque, Florencia Daniela
Caprile, Fernando
Stefani, Fernando Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fluorescence
Single Molecule Localization Microscopy
MINFLUX
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
topic Fluorescence
Single Molecule Localization Microscopy
MINFLUX
https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
description Single-molecule localization microscopy (SMLM) has become an essential tool to investigate phenomena at the nanoscale. Among the different SMLM approaches, methods that interrogate the molecular position with an intensity minimum, such as minimal emission fluxes (MINFLUX) or the more recent raster scanning a minimum of light (RASTMIN), stand out for reaching true molecular resolution. To implement these methods, the phase of the excitation beam needs to be modulated to obtain a focus with a central minimum, i.e., a so-called toroidal or doughnut-shaped focus. In this tutorial,we explain the basis and experimental tricks to generate and optimize such beams, particularly in raster-scanning microscopes.
publishDate 2023
dc.date.none.fl_str_mv 2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228958
Lopez, Lucía Fernanda; Masullo, Luciano Andrés; Szalai, Alan Marcelo; Edorna, Florencia Belen; Choque, Florencia Daniela; et al.; Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial; Optical Society of America; Journal of the Optical Society of America B-Optical Physics; 40; 4; 4-2023; 103-110
0740-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228958
identifier_str_mv Lopez, Lucía Fernanda; Masullo, Luciano Andrés; Szalai, Alan Marcelo; Edorna, Florencia Belen; Choque, Florencia Daniela; et al.; Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial; Optical Society of America; Journal of the Optical Society of America B-Optical Physics; 40; 4; 4-2023; 103-110
0740-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://opg.optica.org/josab/fulltext.cfm?uri=josab-40-4-C103&id=528677
info:eu-repo/semantics/altIdentifier/doi/10.1364/JOSAB.482413
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Optical Society of America
publisher.none.fl_str_mv Optical Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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