Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long am...

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Autores: Kucinskaite Kodze, Indre, Petraityte Burneikiene, Rasa, Zvirbliene, Aurelija, Hjelle, Brian, Medina, Rafael A., Gedvilaite, Alma, Razanskiene, Ausra, Schmidt Chanasit, Jonas, Mertens, Marc, Padula, Paula Julieta, Sasnauskas, Kestutis, Ulrich, Rainer G.
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2011
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/192109
Acesso em linha:http://hdl.handle.net/11336/192109
Access Level:Acceso aberto
Palavra-chave:Antibodies
Monoclonal Antibodies
Viral Nucleocapsid Proteins
hantavirus
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
spellingShingle Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
Kucinskaite Kodze, Indre
Antibodies
Monoclonal Antibodies
Viral Nucleocapsid Proteins
hantavirus
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_full Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_fullStr Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_full_unstemmed Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_sort Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
dc.creator.none.fl_str_mv Kucinskaite Kodze, Indre
Petraityte Burneikiene, Rasa
Zvirbliene, Aurelija
Hjelle, Brian
Medina, Rafael A.
Gedvilaite, Alma
Razanskiene, Ausra
Schmidt Chanasit, Jonas
Mertens, Marc
Padula, Paula Julieta
Sasnauskas, Kestutis
Ulrich, Rainer G.
author Kucinskaite Kodze, Indre
author_facet Kucinskaite Kodze, Indre
Petraityte Burneikiene, Rasa
Zvirbliene, Aurelija
Hjelle, Brian
Medina, Rafael A.
Gedvilaite, Alma
Razanskiene, Ausra
Schmidt Chanasit, Jonas
Mertens, Marc
Padula, Paula Julieta
Sasnauskas, Kestutis
Ulrich, Rainer G.
author_role author
author2 Petraityte Burneikiene, Rasa
Zvirbliene, Aurelija
Hjelle, Brian
Medina, Rafael A.
Gedvilaite, Alma
Razanskiene, Ausra
Schmidt Chanasit, Jonas
Mertens, Marc
Padula, Paula Julieta
Sasnauskas, Kestutis
Ulrich, Rainer G.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antibodies
Monoclonal Antibodies
Viral Nucleocapsid Proteins
hantavirus
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Antibodies
Monoclonal Antibodies
Viral Nucleocapsid Proteins
hantavirus
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192109
Kucinskaite Kodze, Indre; Petraityte Burneikiene, Rasa; Zvirbliene, Aurelija; Hjelle, Brian; Medina, Rafael A.; et al.; Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples; Springer Wien; Archives of Virology; 156; 3; 3-2011; 443-456
0304-8608
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192109
identifier_str_mv Kucinskaite Kodze, Indre; Petraityte Burneikiene, Rasa; Zvirbliene, Aurelija; Hjelle, Brian; Medina, Rafael A.; et al.; Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples; Springer Wien; Archives of Virology; 156; 3; 3-2011; 443-456
0304-8608
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00705-010-0879-6
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00705-010-0879-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Wien
publisher.none.fl_str_mv Springer Wien
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196139244748800
spelling Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samplesKucinskaite Kodze, IndrePetraityte Burneikiene, RasaZvirbliene, AurelijaHjelle, BrianMedina, Rafael A.Gedvilaite, AlmaRazanskiene, AusraSchmidt Chanasit, JonasMertens, MarcPadula, Paula JulietaSasnauskas, KestutisUlrich, Rainer G.AntibodiesMonoclonal AntibodiesViral Nucleocapsid Proteinshantavirushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection.Fil: Kucinskaite Kodze, Indre. Vilnius University; LituaniaFil: Petraityte Burneikiene, Rasa. Vilnius University; LituaniaFil: Zvirbliene, Aurelija. Vilnius University; LituaniaFil: Hjelle, Brian. University Of New Mexico. School Of Medicine; Estados UnidosFil: Medina, Rafael A.. University Of New Mexico. School Of Medicine; Estados UnidosFil: Gedvilaite, Alma. Vilnius University; LituaniaFil: Razanskiene, Ausra. Vilnius University; LituaniaFil: Schmidt Chanasit, Jonas. No especifíca;Fil: Mertens, Marc. No especifíca;Fil: Padula, Paula Julieta. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sasnauskas, Kestutis. Vilnius University; LituaniaFil: Ulrich, Rainer G.. No especifíca;Springer Wien2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192109Kucinskaite Kodze, Indre; Petraityte Burneikiene, Rasa; Zvirbliene, Aurelija; Hjelle, Brian; Medina, Rafael A.; et al.; Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples; Springer Wien; Archives of Virology; 156; 3; 3-2011; 443-4560304-8608CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00705-010-0879-6info:eu-repo/semantics/altIdentifier/doi/10.1007/s00705-010-0879-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:16:08Zoai:ri.conicet.gov.ar:11336/192109instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:16:08.724CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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